A5100458358- Vibrio bacteria research studies
- Aquaculture disease management and microbiota
- Escherichia coli research studies
- Immune Response and Inflammation
- Antibiotic Resistance in Bacteria
- Enterobacteriaceae and Cronobacter Research
- Child Nutrition and Water Access
- Listeria monocytogenes in Food Safety
- Immune cells in cancer
- Reproductive System and Pregnancy
- Experimental Behavioral Economics Studies
- Urinary Tract Infections Management
- Viral gastroenteritis research and epidemiology
- Tryptophan and brain disorders
- T-cell and B-cell Immunology
- Salmonella and Campylobacter epidemiology
- Yersinia bacterium, plague, ectoparasites research
- Amino Acid Enzymes and Metabolism
- Ethics in Business and Education
- Probiotics and Fermented Foods
- Mosquito-borne diseases and control
- Influenza Virus Research Studies
- Hepatitis B Virus Studies
- Urinary Bladder and Prostate Research
- Cell Image Analysis Techniques
Tianjin Agricultural University
2022
Hanshan Normal University
2022
Brigham and Women's Hospital
2016-2021
Harvard University
2014-2021
Jinshan Hospital of Fudan University
2017-2021
Zhejiang A & F University
2019
Sichuan University
2019
Hubei Provincial Center for Disease Control and Prevention
2015-2017
First Affiliated Hospital Zhejiang University
2017
Second Affiliated Hospital of Xi'an Jiaotong University
2017
The aim of this study was to investigate the prevalence polymyxin resistance gene mcr-1 in Enterobacteriaceae from environmental water sources Hangzhou, China. Colistin-resistant bacteria were isolated samples using an enrichment broth culture method, screened for mcr-1, and then analyzed location transferability Isolates positive further examined determine their susceptibility profiles presence additional genes. Twenty-three mcr-1-positive isolates (16 Escherichia coli, two Citrobacter...
C5a receptor 1 (C5aR1) is a G protein-coupled for and also an N-linked glycosylated protein. In addition to myeloid cells, C5aR1 expressed on epithelial cells. this study, we examined the role of in bacterial adhesion/colonization renal tubular epithelium addressed underlying mechanisms role. We show that acute kidney infection was significantly reduced mice with genetic deletion or through pharmacologic inhibition following bladder inoculation uropathogenic E. coli (UPEC). This associated...
Infections caused by drug-resistant "superbugs" pose an urgent public health threat due to the lack of effective drugs; however, certain mammalian proteins with intrinsic antibacterial activity might be underappreciated. Here, we reveal property against Gram-negative bacteria for factors VII, IX and X, three well-established roles in initiation coagulation cascade. These exert function via their light chains (LCs). Unlike many agents that target cell metabolism or cytoplasmic membrane, LCs...
Oral cholera vaccines (OCVs) are being increasingly employed, but current killed formulations generally require multiple doses and lack efficacy in young children. We recently developed a new live-attenuated OCV candidate (HaitiV) derived from Vibrio cholerae strain isolated during the 2010 Haiti epidemic. HaitiV exhibited an unexpected probiotic-like activity infant rabbits, preventing intestinal colonization disease by wild-type V. before onset of adaptive immunity. However, it remained...
Vibrio vulnificus (V. vulnificus), a Gram-negative marine bacterium, can cause life-threatening primary septicemia, especially in patients with liver diseases. How V. affects the and how it acts on macrophages are not well understood. In this report, we demonstrated that infection causes strong inflammatory response, marked expansion of liver-resident macrophages, damage mice. We further activates mTOR inhibition differentially regulates induced responses, suggesting possibility targeting as...
The O1 serogroup of Vibrio cholerae causes pandemic cholera and is divided into the Ogawa Inaba serotypes. O-antigen V. cholerae's immunodominant antigen, two serotypes, which differ by presence or absence a terminally methylated O-antigen, likely influence development immunity to oral vaccines (OCVs). However, there no consensus regarding relative immunological potency each serotype, in part because previous studies relied on genetically heterogeneous strains. Here, we engineered matched...
Abstract Oral cholera vaccines (OCVs) are being increasingly employed, but current killed formulations generally require multiple doses and lack efficacy in young children. We recently developed a new live-attenuated OCV candidate (HaitiV) derived from Vibrio cholerae strain isolated during the 2010 Haiti epidemic. HaitiV exhibited an unexpected probiotic-like activity infant rabbits, preventing intestinal colonization disease by wild-type V. before onset of adaptive immunity. However, it...
Listeria monocytogenes possesses the highest number of leucine-rich repeat (LRR)-containing proteins among all Gram-positive bacteria; these LRR-containing molecules are known as ‘internalin’ family. To understand functions largely uncharacterized molecules, we constructed seven deletion mutants in L. H7858 strain targeting genes this family and tested their virulence. Among mutants, ΔLMOh7858_0369 ΔLMOh7858_2546 showed significantly impaired invasiveness HepG2 cells. We further virulence...
Vibrio vulnificus (V. vulnificus) is an estuarine bacterium that capable of causing rapidly fatal infection in humans. Proper polarization and bactericidal activity macrophages play essential roles defending against invading pathogens. How limit V. remains not well understood. Here we report tuberous sclerosis complex 1 (TSC1) crucial for the regulation vulnificus-induced macrophage polarization, bacterial clearance, cell death. Mice with myeloid-specific deletion TSC1 exhibit a significant...
Abstract The mucin Muc2 is a major constituent of the mucus layer that covers intestinal epithelium and creates barrier between epithelial cells luminal commensal or pathogenic microorganisms. Gram-positive food-borne pathogen Listeria monocytogenes can cause enteritis also disseminate from intestine to give rise systemic disease. L. bind Muc2, but influence on colonization dissemination has not been explored. Here, we used an orogastric infection model investigate role in host defense...
Abstract The O1 serogroup of Vibrio cholerae causes pandemic cholera and is divided into Ogawa Inaba serotypes. O-antigen V. cholerae’s immunodominant antigen, the two serotypes, which differ by presence or absence a terminally methylated O-antigen, likely influence development immunity to oral vaccines (OCVs). However, there no consensus regarding relative immunological potency each serotype, in part because previous studies relied on genetically heterogenous strains. Here, we engineered...
Abstract Background Vibrio vulnificus (V. vulnificus) is a gram-negative opportunistic pathogen that causes lethal infections in humans. hemolysin (VVH) key virulence factor exhibits strong hemolytic and cytolytic activities contributes to the pathogen's invasion, vasodilatation, septic shock. Most of studies so far have focused on VVH's activity against cell lines derived from host cells. However, VVH primary macrophages still unclear. In addition, although it known induces apoptosis via...
Objective To test the pathogeny of Vibrio cholerae strains epidemic in Jinzhou Hubei Province. Methods Traditional methods biochemistry, immobilization test, string and typing blood serum were used to 6 isolated. The virulence gene which was cholera enterotoxin (ct) detected by PCR. whole genome DNA finger print confirmed analyzed pulsed field gel electrophoresis (PFGE) after digestion respectively with two enzymes Not Ⅰ Sfi Ⅰ. fingerprints for clusters. Results The all O139...
Objective To understand how Vibrio vulnificus hemolysin (VvhA) affects the viability of murine liver CD4+ T cells as well its effects on numbers mitochondria and expression CD62L. Methods The primary monocytes (MNs) were isolated from C57BL/6 mice then treated with recombinant VvhA (rVvhA) for 6 hours in vitro. The CD62L measured by staining anti-mouse CD4, CD8, CD44, cell fluorescent dye or antibody. Moreover, simply incubated MitoTracker JC-1 probes to label mitochondrial membrane...