A5023966326- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Immune Response and Inflammation
- Congenital heart defects research
- Hematopoietic Stem Cell Transplantation
- Hepatitis B Virus Studies
- Mesenchymal stem cell research
- Pluripotent Stem Cells Research
- Immune cells in cancer
- Single-cell and spatial transcriptomics
- Cytomegalovirus and herpesvirus research
- Asthma and respiratory diseases
- Cancer Immunotherapy and Biomarkers
- IL-33, ST2, and ILC Pathways
- S100 Proteins and Annexins
- Escherichia coli research studies
- Angiogenesis and VEGF in Cancer
- Monoclonal and Polyclonal Antibodies Research
- Cardiac Fibrosis and Remodeling
- vaccines and immunoinformatics approaches
- Tissue Engineering and Regenerative Medicine
- CRISPR and Genetic Engineering
- Cell Adhesion Molecules Research
Technical University of Munich
2013-2022
Helmholtz Zentrum München
2008-2022
Institute of Medical Microbiology and Hygiene
2013-2022
German Center for Infection Research
2019-2021
Institut für Medizinische Informatik, Biometrie und Epidemiologie
2015-2020
Klinikum rechts der Isar
2004-2016
Center for Environmental Health
2009
Institute for Environment and Human Security
2007
Deutsches Herzzentrum München
2007
University of Würzburg
2007
Several recent studies have demonstrated that T-helper cell-dependent events during the initial priming period are required for generation of CD8(+) T cell-mediated protective immunity. The underlying mechanisms this phenomenon not yet been determined, mostly because difficulties in studying memory cells or their precursor populations at early stages immune responses. We identified IL-7 receptor (CD127) surface expression as a marker long-living cells, most importantly allowing distinction...
Type I IFN production in response to the DNA virus herpes simplex type-1 (HSV-1) is essential controlling viral replication. We investigated whether plasmacytoid dendritic cells (pDC) were major tissue source of IFN-α, and IFN-α HSV-1 depended on Toll-like receptor 9 (TLR9). Total spleen or bone marrow (BM) cells, fractions thereof, including highly purified pDC, from WT, TLR9, MyD88 knockout mice stimulated with known ligands for TLR9 active HSV-1. pDC freshly isolated both BM...
The accumulation of smooth muscle and endothelial cells is essential for remodeling repair injured blood vessel walls. Bone marrow–derived progenitor have been implicated in vascular remodeling; however, the mechanisms underlying their recruitment to site injury remain elusive. Here, using real-time vivo fluorescence microscopy, we show that platelets provide critical signal recruits CD34+ bone marrow c-Kit+ Sca-1+ Lin− sites injury. Correspondingly, specific inhibition platelet adhesion...
A core feature of protective T cell responses to infection is the robust expansion and diversification naïve antigen-specific populations into short-lived effector long-lived memory subsets. By means in vivo fate mapping, we found a striking variability immune derived from individual CD8(+) cells show that acute recall immunity requires initial recruitment multiple precursors. Unbiased mathematical modeling identifies random integration differentiation division events as driving force behind...
Hyperactivation of immune cells by bacterial products through toll-like receptors (TLRs) is thought as a causative mechanism septic shock pathology. Infections with Gram-negative or Gram-positive bacteria provide TLR2-specific agonists and are the major cause severe sepsis. In order to intervene in TLR2-driven toxemia, we raised mAb’s against extracellular domain TLR2. Surface plasmon resonance analysis showed direct specific interaction TLR2 immunostimulatory lipopeptide, which was blocked...
Alternatively polarized macrophages (Mϕ) shape the microenvironment of hepatocellular carcinoma (HCC) and temper anticancer immune responses. We investigated if sorafenib alters HCC by restoring classical macrophage polarization triggering tumor-directed natural killer (NK) cell In vivo experiments were conducted with (25 mg/kg)-treated C57BL/6 wildtype as well hepatitis B virus (HBV) lymphotoxin transgenic mice without HCC. Monocyte-derived Mϕ or tumor-associated (TAM) isolated from tissue...
In many solid tumors, cancer stem cells (CSC) represent a population with tumor-initiating, self-renewal, and differentiation potential, which can be identified by surface protein markers. No generally applicable markers are yet known for renal cell carcinoma (RCC). Two RCC lines (RCC-26, RCC-53) were found to differ widely in their capacity form spheres vitro establish tumors mice, potentially reflecting differences CSC content. A subpopulation expressing the CXC chemokine receptor 4...
Monocytes are key players in atherosclerotic. Human monocytes display a considerable heterogeneity and at least three subsets can be distinguished. While the role of monocyte subset has already been well investigated coronary artery disease (CAD), knowledge about their peripheral occlusive (PAOD) still is limited. Therefore, we aimed to investigate patients with PAOD. Peripheral blood was obtained from 143 suffering PAOD (Rutherford stage I VI) were identified by flow cytometry: CD14++CD16-...
Upon viral infection, natural killer (NK) cells expressing certain germline-encoded receptors are selected, expanded, and maintained in an adaptive-like manner. Currently, these thought to differentiate along a common pathway. However, by fate mapping of single NK upon murine cytomegalovirus (MCMV) we identified two distinct cell lineages that contributed responses. One was equivalent conventional (cNK) while the other transcriptionally similar type 1 innate lymphoid (ILC1s). ILC1-like...
The membrane-bound chemokine fractalkine (CX3CL1) is expressed on various cell types such as activated endothelial cells and has been implicated in the inflammatory process of atherosclerosis. aim present study was to dissect role leukocyte recruitment inflamed endothelium under arterial shear forces.With use immunofluorescence laminar flow assays, shows that human umbilical vein stimulated with tumor necrosis factor-alpha interferon-gamma abundantly express CX3CL1 promote substantial...
Abstract Covalent linkage of immunostimulatory CpG-DNA to OVA (CpG-OVA complex) results in CpG-DNA-aided cross-presentation by dendritic cells (DCs). In this study, we analyzed the thesis that CpG-OVA complexes may be cross-presented B route internalized Ag into class I MHC presentation pathway. First, describe conjugation enhances up 40-fold internalization cells, which turn generate CD8 T cell epitope SIINFEKL complexed I, albeit less efficiently than DCs. Furthermore, upon...
Activation of interferon regulatory factor (IRF)-3 and/or IRF-7 drives the expression antiviral genes and production alpha/beta IFN, a hallmark responses triggered by Toll-like receptors (TLR). Here we describe novel signaling pathway operating in myeloid (m) dendritic cells (DC) macrophages that does not require IRF-3 but is driven IRF-1. IRF-1 together with differentiation 88 (MyD88) or IL-1 receptor-associated kinase (IRAK)-1 IFN-beta promoter activation. physically interacted MyD88...
Ectopic expression of defined sets genetic factors can reprogram somatic cells to create induced pluripotent stem (iPS) cells. The capacity direct human iPS specific differentiated lineages and their progenitor populations be used for disease modeling, drug discovery, eventually autologous cell replacement therapies. During mouse cardiogenesis, the major mature heart, cardiomyocytes, smooth muscle cells, endothelial arise from a common, multipotent cardiovascular expressing transcription...
During cardiogenesis, most myocytes arise from cardiac progenitors expressing the transcription factors Isl1 and Nkx2-5. Here, we show that a direct repression of by Nkx2-5 is necessary for proper development ventricular myocardial lineage. Overexpression in mouse embryonic stem cells (ESCs) delayed specification inhibited expression its downstream targets Isl1(+) precursors. Embryos deficient lineage failed to downregulate protein cardiomyocytes heart tube. We demonstrated directly binds an...