A5089969889- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Liver Disease Diagnosis and Treatment
- Cancer, Lipids, and Metabolism
- Inflammatory mediators and NSAID effects
- Diabetes and associated disorders
- Cytomegalovirus and herpesvirus research
- Diet, Metabolism, and Disease
- Chemokine receptors and signaling
- Pancreatic function and diabetes
- Hepatitis B Virus Studies
- CAR-T cell therapy research
- Immune Response and Inflammation
- Liver Diseases and Immunity
- IL-33, ST2, and ILC Pathways
- Receptor Mechanisms and Signaling
- Peroxisome Proliferator-Activated Receptors
- Cancer, Hypoxia, and Metabolism
Technical University of Munich
2020-2025
Klinikum rechts der Isar
2020-2023
University of Bonn
2020
Type 1 conventional dendritic cells (cDC1) can support T cell responses within tumors but whether this determines protective versus ineffective anti-cancer immunity is poorly understood. Here, we use imaging-based deep learning to identify intratumoral cDC1-CD8+ clustering as a unique feature of immunity. These clusters form selectively in stromal tumor regions and constitute niches which cDC1 activate TCF1+ stem-like CD8+ cells. We distinct population immunostimulatory CCR7neg that produce...
Type 1 conventional dendritic cells (cDC1s) are critical for anti-cancer immunity. Protective immunity is thought to require cDC1s sustain T cell responses within tumors, but it poorly understood how this function regulated and whether its subversion contributes immune evasion. Here, we show that tumor-derived prostaglandin E2 (PGE2) programmed a dysfunctional state in intratumoral cDC1s, disabling their ability locally orchestrate CD8+ responses. Mechanistically, cAMP signaling downstream...
Abstract Cancer-specific TCF1 + stem-like CD8 T cells can drive protective anticancer immunity through expansion and effector cell differentiation 1–4 ; however, this response is dysfunctional in tumours. Current cancer immunotherapies 2,5–9 promote responses some but not all patients. This variation points towards currently ill-defined mechanisms that limit cell-mediated immunity. Here we demonstrate tumour-derived prostaglandin E2 (PGE 2 ) restricts the proliferative of within tumours,...
Upon viral infection, natural killer (NK) cells expressing certain germline-encoded receptors are selected, expanded, and maintained in an adaptive-like manner. Currently, these thought to differentiate along a common pathway. However, by fate mapping of single NK upon murine cytomegalovirus (MCMV) we identified two distinct cell lineages that contributed responses. One was equivalent conventional (cNK) while the other transcriptionally similar type 1 innate lymphoid (ILC1s). ILC1-like...
Abstract Chronic hepatitis B virus (HBV) infection affects 300 million patients worldwide 1,2 , in whom virus-specific CD8 T cells by still ill-defined mechanisms lose their function and cannot eliminate HBV-infected hepatocytes 3–7 . Here we demonstrate that a liver immune rheostat renders refractory to activation leads loss of effector functions. In preclinical models persistent with hepatotropic viruses such as HBV, dysfunctional CXCR6 + accumulated the and, characteristic hallmark,...
This article is part of the Dendritic Cell Guidelines series, which provides a collection state-of-the-art protocols for preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy, and functional characterization mouse human dendritic cells (DC) from lymphoid organs various non-lymphoid tissues. Here, we provide detailed procedures variety multiparameter microscopy imaging methods to explore spatial organization DC in tissues dissect how migrate, communicate,...
A high fat Western-style diet leads to hepatic steatosis that can progress steatohepatitis and ultimately cirrhosis or liver cancer. The mechanism the development of upon nutritional overload is complex only partially understood. Using click chemistry-based metabolic tracing microscopy, we study interaction between Kupffer cells hepatocytes ex vivo. In early phase steatosis, alone do not display significant deviations in fatty acid metabolism. However, co-cultures supernatant transfer...
Natural killer (NK) cells are critical for anti-metastatic immunity and can eliminate metastasizing tumor within circulation sites of metastatic seeding. Here, we show that disseminated (DTCs) colonizing the mouse lung secrete prostaglandin E2 (PGE