A5083497801- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Viral Infectious Diseases and Gene Expression in Insects
- Mesenchymal stem cell research
- Osteoarthritis Treatment and Mechanisms
- Cancer Research and Treatments
- CAR-T cell therapy research
- CRISPR and Genetic Engineering
- Cancer, Hypoxia, and Metabolism
- Angiogenesis and VEGF in Cancer
- Viral gastroenteritis research and epidemiology
- Advanced biosensing and bioanalysis techniques
- Viral Infections and Immunology Research
- Periodontal Regeneration and Treatments
- Chemokine receptors and signaling
- Animal Genetics and Reproduction
- Radiopharmaceutical Chemistry and Applications
- Bacteriophages and microbial interactions
- Immune Cell Function and Interaction
- Pluripotent Stem Cells Research
- Knee injuries and reconstruction techniques
- Medical Imaging Techniques and Applications
- Herpesvirus Infections and Treatments
- Immunotherapy and Immune Responses
- MicroRNA in disease regulation
Technical University of Munich
2015-2025
Klinikum rechts der Isar
2013-2025
Ludwig-Maximilians-Universität München
2003-2012
Helmholtz Zentrum München
2010
Mahidol University
2010
München Klinik
2008-2009
Universidad de Granada
2003
Klinik und Poliklinik für Nuklearmedizin
2000
McMaster University
1995-1998
University of Konstanz
1991-1993
Adenoviruses are attractive vectors for the delivery of foreign genes into mammalian cells gene therapy. However, current retain many viral that, when expressed at low levels, contribute to induction a host immune response against transduced cells. We have developed helper-dependent packaging system production that large regions genome deleted. Helper viruses were constructed with signals flanked by lox P sites so in 293 stably express Cre recombinase (293Cre), signal was efficiently...
Type 1 conventional dendritic cells (cDC1) can support T cell responses within tumors but whether this determines protective versus ineffective anti-cancer immunity is poorly understood. Here, we use imaging-based deep learning to identify intratumoral cDC1-CD8+ clustering as a unique feature of immunity. These clusters form selectively in stromal tumor regions and constitute niches which cDC1 activate TCF1+ stem-like CD8+ cells. We distinct population immunostimulatory CCR7neg that produce...
Type 1 conventional dendritic cells (cDC1s) are critical for anti-cancer immunity. Protective immunity is thought to require cDC1s sustain T cell responses within tumors, but it poorly understood how this function regulated and whether its subversion contributes immune evasion. Here, we show that tumor-derived prostaglandin E2 (PGE2) programmed a dysfunctional state in intratumoral cDC1s, disabling their ability locally orchestrate CD8+ responses. Mechanistically, cAMP signaling downstream...
Transgenic mice expressing the polyomavirus (PyV) middle T antigen (MT) develop multifocal mammary tumors which frequently metastasize to lung. The potent transforming activity of PyV MT is correlated with its capacity activate and associate a number signaling molecules, including Src family tyrosine kinases, 85-kDa homology 2 subunit phosphatidylinositol 3′ (PI-3′) kinase, Shc adapter protein. To uncover role these proteins in MT-mediated tumorigenesis, we have generated transgenic that...
We have constructed replication-defective human adenovirus (Ad) type 5 vectors containing the gene for Cre recombinase from bacteriophage P1 under control of cytomegalovirus immediate-early promoter (AdCre). Expression protein was detected in replication-permissive (293) and nonpermissive (MRC5) cell lines, its biochemical activity demonstrated a cell-free recombination assay using plasmid two loxP sites. To study Cre-mediated an intracellular system, we Ad vector (AdMA19) luciferase cDNA...
Experiments were performed with cold atmospheric plasma (CAP) to inactivate adenovirus, a non-enveloped double stranded DNA virus, in solution. The source used was surface micro-discharge technology operating air. Various diagnostic measurements and tests order determine the efficacy of CAPs understand inactivation mechanism(s). Different stages adenovirus ‘life cycle’ investigated—infectivity gene expression as well viral replication spread. Within 240 s CAP treatment, up 6 decimal log...
Mesenchymal stromal cells isolated from bone marrow (MSC) represent an attractive source of adult stem for regenerative medicine. However, thorough research is required into their clinical application safety issues concerning a risk potential neoplastic degeneration in process MSC propagation cell culture therapeutic applications. Expansion protocols could preselect with elevated levels growth-promoting transcription factors oncogenic potential, such as c-MYC. We addressed the question...
There is a need for in vivo monitoring of cell engraftment and survival after cardiac transplantation therapy. This study assessed the feasibility usefulness combined PET MRI transplantation. <b>Methods:</b> Human endothelial progenitor cells (HEPCs), derived from CD34+ mononuclear umbilical cord blood, were retrovirally transduced with sodium iodide symporter (NIS) gene reporter imaging by <sup>124</sup>I-PET labeled iron oxides visualization MRI. Imaging histologic analysis performed on 3...
Gastric cancer (GC) is still one of the most common causes cancer-related death worldwide, which mainly attributable to late diagnosis and poor treatment options. Infection with Helicobacter pylori, different environmental factors genetic alterations are known influence risk developing gastric tumors. However, molecular mechanisms involved in carcinogenesis not fully understood, making it difficult design targeted therapeutic approaches. Aberrant expression specific differentiation marker...
The influence of stem cells and lentiviral expression basic fibroblastic growth factor (bFGF) on tendon healing remodelling was investigated in an in-vivo long-term (12 weeks) rat Achilles defect model. In sixty male Lewis rats, complete defects (2.4 mm) were created either left untreated (PBS) or treated by injection lentivirally expressing the enhanced green fluorescence marker gene eGFP (MSC-LV-eGFP) fibroblast bFGF (MSC-LV-bFGF). Tendons harvested after 12 weeks underwent biomechanical...
Abstract Evasion of programmed cell death represents a critical form oncogene addiction in cancer cells. Understanding the molecular mechanisms underpinning survival despite oncogenic stress could provide basis for potential therapeutic interventions. Here we explore role pro-survival genes integrity during clonal evolution non-small lung (NSCLC). We identify gains MCL-1 at high frequency multiple independent NSCLC cohorts, occurring both clonally and subclonally. Clonal loss functional TP53...
Background— Radionuclide imaging of reporter gene expression may be useful for noninvasive monitoring clinical cardiac therapy. Experience until now, however, has been limited to small animals. Methods and Results— To evaluate feasibility in a clinically applicable setting, pigs were studied by conventional positron emission tomography (PET) 2 days after regional intramyocardial injection control adenovirus or carrying herpesviral thymidine kinase (HSV1-tk). Myocardial blood flow was...
Oncolytic adenoviruses rank among the most promising innovative agents in cancer therapy. We examined potential of boosting efficacy oncolytic adenovirus dl520 by associating it with magnetic nanoparticles and magnetic-field-guided infection multidrug-resistant (MDR) cells vitro upon intratumoral injection vivo. The virus was complexed self-assembly core−shell having a magnetite core about 10 nm stabilized shell containing 68 mass % lithium 3-[2-(perfluoroalkyl)ethylthio]propionate) 32 25...
Tumor necrosis factor alpha (TNFα) is a potent antitumoral cytokine, either killing tumor cells directly or affecting the vasculature leading to enhanced accumulation of macromolecular drugs. Due dose limiting side effects systemic administration TNFα protein at therapeutically active doses precluded. With gene vectors, restricted expression can be achieved and in principle synergize with chemotherapy. Synthetic carriers based on polyamines were intravenously injected, which passively...