A5056947461- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Forensic Toxicology and Drug Analysis
- Veterinary Pharmacology and Anesthesia
- Hormonal and reproductive studies
- Medical Imaging Techniques and Applications
- Evolutionary Psychology and Human Behavior
- Ion-surface interactions and analysis
- Mitochondrial Function and Pathology
- Nuclear Physics and Applications
- Neuroscience and Neuropharmacology Research
- Stress Responses and Cortisol
- Analytical Chemistry and Chromatography
- Molecular Biology Techniques and Applications
- Pancreatic function and diabetes
- Analytical chemistry methods development
- Biochemical effects in animals
- Forensic and Genetic Research
- Free Radicals and Antioxidants
- Metabolism, Diabetes, and Cancer
- Race, Genetics, and Society
- Advanced Proteomics Techniques and Applications
- Demographic Trends and Gender Preferences
- Advanced Biosensing Techniques and Applications
- Laser Applications in Dentistry and Medicine
Maastricht University
2021-2025
KU Leuven
2017-2021
Recently, a novel technology was published, utilizing the strengths of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) and immunohistochemistry (IHC), achieving highly multiplexed, targeted biomolecules in tissue. This new technique, called MALDI-IHC, opened up workflows to target molecules interest using MALDI-MSI that are usually by standard IHC. In this paper, utility MALDI-IHC its complementarity with untargeted on-tissue bottom-up spatial proteomics...
We introduce a novel approach for comprehensive molecular profiling in biological samples. Our single-section methodology combines quantitative mass spectrometry imaging (Q-MSI) and single step extraction protocol enabling lipidomic proteomic liquid chromatography tandem (LC-MS/MS) analysis on the same tissue area. The integration of spatially correlated data section allows interpretation landscape. Comparing Q-MSI Q-LC-MS/MS quantification results sheds new light effect MSI related sample...
Abstract Chronic hepatitis B virus (HBV) infection affects 300 million patients worldwide 1,2 , in whom virus-specific CD8 T cells by still ill-defined mechanisms lose their function and cannot eliminate HBV-infected hepatocytes 3–7 . Here we demonstrate that a liver immune rheostat renders refractory to activation leads loss of effector functions. In preclinical models persistent with hepatotropic viruses such as HBV, dysfunctional CXCR6 + accumulated the and, characteristic hallmark,...
Abstract Multimodal bioimaging is a broad term used to describe experimental workflows that employ two or more different imaging modalities. Such approaches have been in use across life science domains for several years but these remain relatively limited scope, part due the complexity of undertaking types analysis. Expanding encompass diverse, emerging technology holds potential revolutionize our understanding spatial biology. In this perspective we reflect on instrument and current use,...
In the past decades, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been applied to a broad range of biological samples, e.g., forensics and preclinical samples. The use MALDI-MSI for analysis bone tissue limited due insulating properties material but more importantly absence proper sample preparation protocol undecalcified tissue. Undecalcified sections are preferred retain integrity as much possible or study tissue-bone bio interface in particular....
Mass spectrometry imaging (MSI) has accelerated our understanding of lipid metabolism and spatial distribution in tissues cells. However, few MSI studies have approached quantitatively those that focused on a single class. We overcome this limitation by using multiclass internal standard (IS) mixture sprayed homogeneously over the tissue surface with concentrations reflect endogenous lipids. This enabled quantitative (Q-MSI) 13 classes subclasses representing almost 200 sum-composition...
Histidine-containing dipeptides (HCDs) are pleiotropic homeostatic molecules with potent antioxidative and carbonyl quenching properties linked to various inflammatory, metabolic, neurological diseases, as well exercise performance. However, the distribution metabolism of HCDs across tissues species still unclear.Using a sensitive UHPLC-MS/MS approach an optimized quantification method, we performed systematic extensive profiling in mouse, rat, human body (in n = 26, 25, 19 tissues,...
Doxorubicin (dox) is an affordable, and highly effective chemotherapeutic agent used in cancer treatment, yet its application known to cause cumulative cardiac renal toxicity. In this study, we employed matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) evaluate the distribution of dox mouse heart kidney after vivo treatment. To end, performed absolute quantification using isotopically labeled form (13C d3-dox) as internal standard. Unfortunately, ion...
As the main inhibitory neurotransmission system, GABAergic system poses an interesting yet underutilized target for molecular brain imaging. While PET imaging of postsynaptic neurons has been accomplished using radiolabeled benzodiazepines targeting GABAA receptor, development presynaptic radioligands GABA transporter 1 (GAT1) unsuccessful thus far. Therefore, we developed a novel GAT1-addressing radioligand and investigated its applicability as tracer in rodents. We selected lipophilic...
Evaluating tissue distribution of Positron Emission Tomography (PET) tracers during their development conventionally involves autoradiography techniques, where radioactive compounds are used for ex vivo visualization and quantification in tissues preclinical stages. Mass Spectrometry Imaging (MSI) offers a potential alternative, providing spatial information without the need radioactivity with similar resolution. This study aimed to optimize MSI sample preparation protocol assessing PET...
Abstract Objective In temporal lobe epilepsy (TLE), detection of the epileptogenic zone predicts a good surgical outcome. When submitted to 18 F‐fluorodeoxyglucose positron emission tomography (PET), some patients display lateralized, focal hypometabolism in (PET+), whereas others appear normometabolic (PET−). However, mechanism behind this metabolic difference remains unclear. This study aimed identify differential molecular mechanisms these patient subtypes. Methods Neocortical and...
Metabolic dysfunction-associated steatotic liver disease (MASLD) is the most common chronic worldwide for which there only one approved treatment. Adenosine monophosphate-activated protein kinase (AMPK) an interesting therapeutic target since it acts as a central regulator of cellular metabolism. Despite efforts to AMPK, no direct activators have yet been treatment this disease. This study investigated effect AMPK activator ATX-304 in preclinical mouse model progressive fatty The data...
Abstract Objective Evolutionary theory has shown that seeking out extrapair paternity (EPP) can be a viable reproductive strategy for both sexes in pair‐bonded species, also humans. As yet, estimates of the contemporary or historical EPP rate human population are still rare. In present study, we estimated Dutch over last 400 years and compared with those obtained other populations to determine evolutionary, cultural, socio‐demographic factors influence cuckoldry behavior. Methods We via...
In forensic toxicology, when extended time has elapsed before discovery of the body, most commonly analyzed specimens are often degraded or not available at all due to decomposition. this case, skeletal tissue may be only specimen left. Nevertheless, very limited research is found on drug disposition in bone, making toxicological interpretation difficult. Since methadone linked almost 50% abuse fatalities Belgium, an easy extraction and quantification method was developed validated...
Abstract Every year, thousands of suspicious deaths are accounted for by an overdose opioids. Occasionally all traditional matrices unavailable due to decomposition. Skeletal tissue may pose a valid alternative. However, reference data on postmortem concentrations in bone and marrow (BM) is sparse. Therefore, liquid chromatography--tandem mass spectrometry method was developed fully validated the analysis four opioids two metabolites (tramadol, O-desmethyltramadol, morphine, fentanyl,...
Abstract Histidine-containing dipeptides (HCDs) are pleiotropic homeostatic molecules linked to inflammatory, metabolic and neurological diseases, as well exercise performance. Using a sensitive UHPLC-MS/MS approach an optimized quantification method, we performed systematic extensive profiling of HCDs in the mouse, rat, human body (in n=26, n=25, n=19 tissues, respectively). Our data show that tissue HCD levels uniquely regulated by carnosine synthase (CARNS1), enzyme was preferentially...
Rationale Matrix‐assisted laser desorption/ionisation‐mass spectrometry imaging (MALDI‐MSI) is a powerful label‐free technique for biomolecule detection (e.g., lipids), within tissue sections across various biological species. However, despite its utility in many applications, the nematode Caenorhabditis elegans not routinely used combination with MALDI‐MSI. The lack of studies exploring spatial distribution biomolecules nematodes likely due to challenges sample preparation. Methods This...
Abstract In recent years, the use of skeletal tissue as an alternative matrix in forensic toxicology has received new interest. cases where extreme decomposition taken place, analysis is often only option left. this article, a fully validated method presented and distribution clomipramine, citalopram, midazolam, metabolites after chronically administration examined within tissue. Rats were dosed with respectively or midazolam. Extracts quantitatively analyzed using liquid...
Abstract Mass spectrometry imaging (MSI) has accelerated the understanding of lipid metabolism and spatial distribution in tissues cells. However, few MSI studies have approached quantitatively those that focus on a single class. Herein, we overcome limitation quantitative (Q-MSI) by using multi-class internal standard mixture is sprayed homogenously over tissue surface with analytical concentrations reflects endogenous brain levels. Using this approach performed Q-MSI for 13 classes...