A5035554409- Cell Image Analysis Techniques
- Glycosylation and Glycoproteins Research
- Reproductive System and Pregnancy
- Estrogen and related hormone effects
- Cellular transport and secretion
- Endoplasmic Reticulum Stress and Disease
- Ovarian function and disorders
- Lipid metabolism and biosynthesis
- Endometriosis Research and Treatment
- Galectins and Cancer Biology
- Advanced Fluorescence Microscopy Techniques
- Reproductive Biology and Fertility
- Advanced Electron Microscopy Techniques and Applications
- Photoacoustic and Ultrasonic Imaging
- Genetics, Bioinformatics, and Biomedical Research
- Single-cell and spatial transcriptomics
- AI in cancer detection
- Wnt/β-catenin signaling in development and cancer
- Digestive system and related health
- Nuclear Structure and Function
- Advanced Biosensing Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Lysosomal Storage Disorders Research
- Interdisciplinary Research and Collaboration
- Infant Nutrition and Health
University of Gothenburg
2014-2024
National Institutes of Health
2022
University of Pisa
2022
Universidad Complutense de Madrid
2022
University of Wisconsin–Madison
2022
National Yang Ming Chiao Tung University
2022
Czech Academy of Sciences
2022
University of Strathclyde
2022
Duke University
2022
Inserm
2022
Abstract MXenes, a young family of 2D transition metal carbides/nitrides, show great potential in electrochemical energy storage applications. Herein, high performance ultrathin flexible solid‐state supercapacitor is demonstrated based on Mo 1.33 C MXene with vacancy ordering an aligned layer structure MXene/poly(3,4‐ethylenedioxythiophene):poly(styrenesulfonic acid) (PEDOT:PSS) composite film posttreated concentrated H 2 SO 4 . The delivers maximum capacitance 568 F cm −3 , ultrahigh...
Abstract Multimodal bioimaging is a broad term used to describe experimental workflows that employ two or more different imaging modalities. Such approaches have been in use across life science domains for several years but these remain relatively limited scope, part due the complexity of undertaking types analysis. Expanding encompass diverse, emerging technology holds potential revolutionize our understanding spatial biology. In this perspective we reflect on instrument and current use,...
Sir2 is a central regulator of yeast aging and its deficiency increases daughter cell inheritance stress- aging-induced misfolded proteins deposited in aggregates inclusion bodies. Here, by quantifying traits predicted to affect aggregate passive manner, we found that diffusion model cannot explain Sir2-dependent failures mother-biased segregation either the small formed Huntingtin, Htt103Q, disease protein or heat-induced Hsp104-associated aggregates. Instead, genetic interaction network...
Abstract In the dynamic landscape of scientific research, imaging core facilities are vital hubs propelling collaboration and innovation at technology development dissemination frontier. Here, we present a collaborative effort led by Global BioImaging (GBI), introducing international recommendations geared towards elevating careers Imaging Scientists in facilities. Despite critical role modern research ecosystems, challenges persist recognising their value, aligning performance metrics...
We have investigated the role for diacylglycerol (DAG) in membrane bud formation Golgi apparatus. Addition of propranolol to specifically inhibit phosphatidate phosphohydrolase (PAP), an enzyme responsible converting phosphatidic acid into DAG, effectively prevents buds. The effect PAP inhibition on membranes is rapid and occurs within 3 min. Removal inhibitor then results a burst buds, vesicles, tubules that peaks 2 inability form buds presence does not appear be correlated with loss...
The impact of the different reconstruction parameters in super-resolution structured illumination microscopy (SIM) on image artifacts is carefully analyzed. These comprise Wiener filter parameter, an apodization function, zero-frequency suppression and modifications optical transfer function. A detailed investigation reconstructed spectrum concluded to be suitable for identifying artifacts. For this purpose, two samples, artificial test slide a more realistic biological system, were used...
A modern day light microscope has evolved from a tool devoted to making primarily empirical observations what is now sophisticated , quantitative device that an integral part of both physical and life science research. Nowadays, microscopes are found in nearly every experimental laboratory. However, despite their prevalent use capturing quantifying scientific phenomena, neither thorough understanding the principles underlying imaging techniques nor appropriate knowledge how calibrate,...
Adult female rats continuously exposed to androgens from prepuberty have reproductive and metabolic features of polycystic ovary syndrome (PCOS). We investigated whether such exposure adversely affects estrous cyclicity the expression distribution gonadotropin-releasing hormone (GnRH), GnRH receptors, corticotrophin-releasing (CRH) in hypothalamus effects are mediated by androgen receptor (AR). also assessed effect low-frequency electro-acupuncture (EA) on those variables. At 21 days age,...
Estrogen receptors (ERs) are members of the nuclear receptor superfamily and involved in regulation fallopian tube functions (i.e., enhancement protein secretion, formation tubal fluid, gamete transport). However, ER subtype-mediated mechanisms underlying these processes have not been completely clarified. Recently, we identified ERbeta expression localization rat tubes, suggesting a potential biological function related to calcium-dependent ciliated beating. Here provide for first time...
OBJECTIVE Previous findings demonstrate that enhanced expression of the forkhead transcription factor Foxc2 in adipose tissue leads to a lean and insulin-sensitive phenotype. These prompted us further investigate role regulation genes fundamental importance for metabolism mitochondrial function. RESEARCH DESIGN AND METHODS The effects on involved mitochondriogenesis function were assessed by quantitative real-time PCR. potential direct transcriptional regulated was tested promoter assays,...
Several ERβ isoforms have been identified in human and rodent tissues, but it is unclear whether each isoform has distinctly different cellular targeting characteristics physiological functions. We investigated the intracellular localization regulatory patterns for rat fallopian tubes. Western blot analysis reveals that two corresponding to ERβ1 ERβ2 are expressed However, predominant form of this tissue. High-resolution confocal imaging immunohistochemical provide ample evidence expression...
Discovered in 1909 by Retzius and described mainly morphology, the cytoplasmic droplet of sperm (renamed here Hermes body) is conserved among all mammalian species but largely undefined at molecular level. Tandem mass spectrometry isolated body from rat epididymal characterized 1511 proteins, 43 which were localized to structure situ light microscopy two quantitative electron localization. Glucose transporter 3 (GLUT-3) glycolytic enzymes, selected membrane traffic cytoskeletal proteins...
Progesterone and its interaction with nuclear progesterone receptors (PR) PR-A PR-B play a critical role in the regulation of female reproductive function all mammals. However, our knowledge possible cellular PR protein isoforms fallopian tube uterus vivo is still very limited. In present study, we revealed that equine chorionic gonadotropin (eCG) treatment resulted time-dependent increase expression both isoforms, reaching maximal level at 48 h tube. Regulation paralleled expression. levels...
The action of interleukin-6 (IL-6) impacts female reproduction. Although IL-6 was recently shown to inhibit cilia activity in human fallopian tubes vitro, the molecular mechanisms underlying signaling tubal function remain elusive. Here, we investigate cellular localization, regulation, and possible two receptors (IL-6Rα gp130) mouse vivo. We show that IL-6Rα is restricted epithelial cells both tubes. Exogenous 17β-estradiol (E 2 ), but not progesterone (P 4 causes a time-dependent decrease...
The molecular basis of changes in structure, cellular location, and function the Golgi apparatus during male germ cell differentiation is unknown. To deduce cognate proteins, we isolated fractions, 1318 proteins were characterized, with 20 localized situ. most abundant protein, GL54D unknown function, characterized as a cell–specific Golgi-localized type II integral membrane glycoprotein. TM9SF3, also was revealed to be universal marker for both somatic cells. During acrosome formation,...
<i>Objectives:</i> The purpose of this study was to assess the value serum levels α-<i>L</i>-fucosidase activity in diagnosis patients with colorectal cancer. <i>Methods:</i> Using a fluorometric method we analyzed preoperative sera from 137 cancer and 232 donors. <i>Results:</i> enzymatic significantly lower (p < 0.001) (4.8 ± 3.09 U/ml) than donors (10.5 5.46 U/ml). ROC curve, ideal cut-off for diagnostic determined be 5.6 U/ml. efficiency...
Clomiphene citrate (CC) therapy for disorders of anovulatory infertility has been linked to an increased frequency tubal ectopic pregnancy. Although CC enhances apoptotic processes in the ovaries, villi, and decidual tissues, its effect on apoptosis fallopian tube is unknown. Here, we show that chronic treatment with induces apoptosis, but not necrosis, through intrinsic mitochondria-dependent signaling pathway vivo. The was specific epithelial cells isthmus, damage reversed 17beta-estradiol...
The anterior pituitary-derived hormone prolactin (PRL) signals through the PRL receptor (PRLR) and is important for female reproductive function in mammals. In contrast to extensive studies of PRLR expression regulation human mouse ovary uterus, mechanisms controlling isoform fallopian tube are poorly understood. Because dynamic interaction hormonal signaling gonadal tissue pituitary or tissues themselves mammals suggests endocrine paracrine expression, we questioned whether differential...