Ulrike Boehm
A5066142568- Cell Image Analysis Techniques
- Research Data Management Practices
- Scientific Computing and Data Management
- Advanced Fluorescence Microscopy Techniques
- Genetics, Bioinformatics, and Biomedical Research
- AI in cancer detection
- Advanced Electron Microscopy Techniques and Applications
- Single-cell and spatial transcriptomics
- Radiomics and Machine Learning in Medical Imaging
- Cellular Mechanics and Interactions
- Biosimilars and Bioanalytical Methods
- Advanced Materials Characterization Techniques
- Embedded Systems Design Techniques
- Optical measurement and interference techniques
- Advanced Biosensing Techniques and Applications
- RNA and protein synthesis mechanisms
- Conferences and Exhibitions Management
- Cellular transport and secretion
- Seismic Imaging and Inversion Techniques
- Education Methods and Technologies
- Machine Learning in Materials Science
- Radiopharmaceutical Chemistry and Applications
- RNA Research and Splicing
- Block Copolymer Self-Assembly
- Advanced Optical Sensing Technologies
Janelia Research Campus
2020-2024
Howard Hughes Medical Institute
2021-2024
Carl Zeiss (Germany)
2022-2024
European Molecular Biology Organization
2023
Imaging Center
2022
Max Planck Institute of Biochemistry
2010
Technische Universität Dresden
2010
A modern day light microscope has evolved from a tool devoted to making primarily empirical observations what is now sophisticated , quantitative device that an integral part of both physical and life science research. Nowadays, microscopes are found in nearly every experimental laboratory. However, despite their prevalent use capturing quantifying scientific phenomena, neither thorough understanding the principles underlying imaging techniques nor appropriate knowledge how calibrate,...
Abstract For quality, interpretation, reproducibility and sharing value, microscopy images should be accompanied by detailed descriptions of the conditions that were used to produce them. Micro-Meta App is an intuitive, highly interoperable, open-source software tool was developed in context 4D Nucleome (4DN) consortium designed facilitate extraction collection relevant metadata as specified recent 4DN-BINA-OME tiered-system Microscopy Metadata specifications. In addition substantially...
Together with the molecular knowledge of genes and proteins, biological images promise to significantly enhance scientific understanding complex cellular systems advance predictive personalized therapeutic products for human health. For this potential be realized, quality-assured image data must shared among labs at a global scale compared, pooled, reanalyzed, thus unleashing untold beyond original purpose which was generated. There are two broad sets requirements enable sharing in life...
Images document scientific discoveries and are prevalent in modern biomedical research. Microscopy imaging particular is currently undergoing rapid technological advancements. However for scientists wishing to publish the obtained images image analyses results, there date no unified guidelines. Consequently, microscopy data publications may be unclear or difficult interpret. Here we present community-developed checklists preparing light analysis publications. These offer authors, readers,...
Inside the cell, proteins essential for signaling, morphogenesis, and migration navigate complex pathways, typically via vesicular trafficking or microtubule-driven mechanisms
1 - ABSTRACT Digital light microscopy provides powerful tools for quantitatively probing the real-time dynamics of subcellular structures. While power modern techniques is undeniable, rigorous record-keeping and quality control are required to ensure that imaging data may be properly interpreted ( ), reproduced reproducibility used extract reliable information scientific knowledge which can shared further analysis value ). Keeping notes on experiments procedures ought straightforward, as...
The application of microscopy in biomedical research has come a long way since Antonie van Leeuwenhoek discovered unicellular organisms. Countless innovations have positioned light as cornerstone modern biology and method choice for connecting omics datasets to their biological clinical correlates. Still, regardless how convincing published imaging data looks, it does not always convey meaningful information about the conditions which was acquired, processed, analyzed. Adequate...
Abstract For the information content of microscopy images to be appropriately interpreted, reproduced, and meet FAIR (Findable Accessible Interoperable Reusable) principles, they should accompanied by detailed descriptions microscope hardware, image acquisition settings, pixel dimensional structure, instrument performance. Nonetheless, thorough documentation imaging experiments is significantly impaired lack community-sanctioned easy-to-use software tools facilitate extraction collection...
To obtain accurate, reproducible, and interpretable data when conducting imaging experiments, it is critical to consider external factors affecting acquisition at various steps of the experimental workflow. Illumination power stability represent two factors, especially comparing fluorescence intensities between images during a time-lapse experiment or experiments performed different times on microscopes. The signal can be generated by types light sources. These sources their coupling...
To obtain accurate, reproducible, and interpretable data when conducting imaging experiments, it is critical to consider external factors affecting acquisition at various steps of the experimental workflow. Illumination power stability represent two factors, especially comparing fluorescence intensities between images during a time-lapse experiment or experiments performed different times on other microscopes. The signal can be generated by types light sources. These sources their coupling...
Extended abstract of a paper presented at Microscopy and Microanalysis 2010 in Portland, Oregon, USA, August 1 – 5, 2010.
Views Icon Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Twitter Facebook Reddit LinkedIn Tools Reprints and Permissions Cite Search Site Citation Ulrike Boehm, Gesche Pospiech, Peggy Martin; Improving Synergies through the Mentoring of Student Teachers during Practical Training in Schools their Integration Applied Research. AIP Conf. Proc. 28 July 2010; 1263 (1): 98–101. https://doi.org/10.1063/1.3479903 Download citation file: Ris (Zotero) Reference...
My presentation at Harvard Medial School about 4Pi microscopy and its derivatives (isoSTED, 4Pi-RESOLFT, 4Pi-SMS, iPALM) in biological research with five question sessions. The includes teaching elements latest results. References of all used figures videos is given. Please feel free to contact Ulrike Boehm case questions.