A5039760968- Cancer, Hypoxia, and Metabolism
- Occupational and environmental lung diseases
- Glycosylation and Glycoproteins Research
- Cell Image Analysis Techniques
- Ubiquitin and proteasome pathways
- Lipid Membrane Structure and Behavior
- Epigenetics and DNA Methylation
- Cancer-related Molecular Pathways
- Cancer Genomics and Diagnostics
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Cell Adhesion Molecules Research
- Erythrocyte Function and Pathophysiology
- Mitochondrial Function and Pathology
- Cellular transport and secretion
- Pancreatic function and diabetes
- Advanced Fluorescence Microscopy Techniques
- Galectins and Cancer Biology
- Blood Coagulation and Thrombosis Mechanisms
- 3D Printing in Biomedical Research
- Monoclonal and Polyclonal Antibodies Research
- Cell death mechanisms and regulation
- Cellular Mechanics and Interactions
- Protease and Inhibitor Mechanisms
- Caveolin-1 and cellular processes
- Platelet Disorders and Treatments
University of Vermont
2016-2025
Imaging Center
2015-2024
European Molecular Biology Organization
2023
Centre National de la Recherche Scientifique
1995-2022
IMDEA Nanoscience
2022
University of Limerick
2018-2022
Molecular Medicine Research Institute
2022
Italian Institute of Technology
2022
University of Pisa
2022
Universidad Complutense de Madrid
2022
The objective was to assess the effect of a new, highly selective beta 3-adrenergic agonist, CL-316,243 (CL) (J. D. Bloom, M. Dutia, B. Johnson, A. Wissner, G. Burns, E. Largis, J. Dolan, and T. H. Claus., Med. Chem. 35: 3081, 1992), on energy balance brown white adipose tissues (BAT WAT, respectively) in young rats eating high-fat diet induce obesity. Chronic treatment with CL increased body temperature 24-h expenditure, mainly by increasing resting metabolic rate. Food intake not altered...
A unique structural feature of the neural cell adhesion molecule N-CAM is presence homopolymers alpha (2----8)-linked sialic acid units. We have used two specific probes for detection poly(sialic acid) in normal human kidney and Wilms tumor: a monoclonal antibody against meningococci group B capsular polysaccharide (homopolymers units), which shows no crossreactivity with polynucleotides denaturated DNA, bacteriophage-induced endosialidases specifically hydrolyzing Additionally, N-CAM,...
Two terminal glycosyltransferases, a sialyltransferase and the blood group A alpha 1,3 N-acetylgalactosaminyltransferase, were found to exhibit differential subcompartmentation in Golgi apparatus of intestinal goblet absorptive cells. As expected from their role glycosylation, two glycosyltransferases products, sialic acid residues substance, localized trans cisternae In contrast, however, they throughout stack adjacent cells, with exception fenestrated first cis cisterna. The results are...
Mitochondria are the main engine that generates ATP through oxidative phosphorylation within respiratory chain. Mitochondrial respiration is regulated according to metabolic needs of cells and can be modulated in response changes. Little known about mechanisms regulate this process. Here, we identify MCJ/DnaJC15 as a distinct cochaperone localizes at mitochondrial inner membrane, where it interacts preferentially with complex I electron transfer We show MCJ impairs formation supercomplexes...
Abstract Airways display robust NF-κB activation and represent targets for anti-inflammatory asthma therapies, but the functional importance of in airway epithelium remains enigmatic. Therefore, transgenic mice were created which is repressed specifically airways (CC10-IκBαSR mice). In response to inhaled Ag, demonstrated significantly ameliorated inflammation, reduced levels chemokines, T cell cytokines, mucus metaplasia, circulating IgE compared with littermate controls. Despite these...
Group V secretory phospholipase A2 (GV sPLA2) has been detected in both human and mouse atherosclerotic lesions. This enzyme potent hydrolytic activity towards phosphatidylcholine-containing substrates, including lipoprotein particles. Numerous studies vitro indicate that hydrolysis of high density lipoproteins (HDL) low (LDL) by GV sPLA2 leads to the formation atherogenic particles potentially proinflammatory lipid mediators. However, there is no direct evidence this promotes processes...
IL-6 plays an important role in determining the fate of effector CD4 cells and cytokines that these produce. Here we identify a novel molecular mechanism by which regulates cell function. We show IL-6-dependent signal facilitates formation mitochondrial respiratory chain supercomplexes to sustain high membrane potential late during activation cells. Mitochondrial hyperpolarization caused is uncoupled from production ATP oxidative phosphorylation. However, it raise levels Ca(2+) Increased...
Abstract During studies on the Golgi apparatus immunolocalization of beta-galactoside alpha 2,6-sialyltransferase in intestinal cells, immunostaining a number post-Golgi structures including mucus droplets and plasma membrane were observed. In order to determine if this labeling was fact due sialyltransferase not carbohydrate-specific antibodies polyclonal antiserum preparation, fusion protein used epitope purify polypeptide-specific antibodies. The affinity purification performed column...
Atomic force microscopy reveal pit‐like structures typically containing three or four, ∼150nm in diameter depressions at the apical plasma membrane live pancreatic acinar cells. Stimulation of secretion causes these to dilate and return their resting size following completion process. Exposure cells cytochalasin B results decreased depression a loss stimulable secretion. It is hypothesized that are fusion pores, where membrane‐bound secretory vesicles dock fuse release vesicular contents....
We report the presence of alpha(2----8)-linked polysialic acid as detected with a monoclonal antibody outside nervous tissue in postnatal developing rat kidney. By immunoblot analysis, reactivity was confined to broad band apparent molecular mass 200-250 kDa. immunohistochemistry, units were found throughout ureteric bud-derived collecting duct system. In nephrons derived from metanephrogenic mesenchyme, only regionally and transiently expressed. Furthermore, expression developmentally...
Beta 1,4-galactosyltransferase (beta 1,4-GT) is a Golgi-resident, type II membrane-bound glycoprotein that functions in the coordinate biosynthesis of complex oligosaccharides. Additionally, beta 1,4-GT has been localized to cell surface variety types and tissues where it proposed function intercellular recognition and/or adhesion. Thus an appropriate molecule be used analyzing molecular basis for retention enzyme Golgi its subsequent or alternative transport surface. Previously we have...
Asbestos causes protracted, dose-dependent increases in steady-state mRNA levels of the proto-oncogenes c-fos and c-jun, AP-1 DNA-binding activity normal rat pleural mesothelial (RPM) cells (1). To determine phenotypic end points overexpression these early response genes by asbestos, both cell proliferation apoptosis were examined confluent RPM exposed to a range concentrations (1.25 10 micrograms/cm2 dish) crocidolite asbestos for 24 48 h. Quantitation pulsed with 5-bromo-2'-deoxyuridine...
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