A5007862294- Pregnancy and preeclampsia studies
- Inflammatory mediators and NSAID effects
- Reproductive System and Pregnancy
- Eicosanoids and Hypertension Pharmacology
- Tissue Engineering and Regenerative Medicine
- Cancer-related Molecular Pathways
- Trypanosoma species research and implications
- Cancer, Stress, Anesthesia, and Immune Response
- Angiogenesis and VEGF in Cancer
- Hormonal Regulation and Hypertension
- Connexins and lens biology
- Asthma and respiratory diseases
- Cell death mechanisms and regulation
- Cell Adhesion Molecules Research
- Research on Leishmaniasis Studies
- Birth, Development, and Health
- Cancer-related molecular mechanisms research
- Chemokine receptors and signaling
- Pulmonary Hypertension Research and Treatments
- 3D Printing in Biomedical Research
- Heat shock proteins research
- Electrospun Nanofibers in Biomedical Applications
- Connective Tissue Growth Factor Research
- Mitochondrial Function and Pathology
- Gestational Diabetes Research and Management
Lankenau Institute for Medical Research
2021-2025
Baruch S. Blumberg Institute
2020-2025
The University of Sydney
2015-2025
University of Aruba
2021-2025
Albert Einstein College of Medicine
2003-2023
Northern Sydney Local Health District
2015-2023
Royal North Shore Hospital
2011-2022
Keogh Institute for Medical Research
2010-2019
Perinatal Institute
2015
Australian National University
2013-2014
The eicosanoid thromboxane A(2) (TXA(2)) is released by activated platelets, monocytes, and the vessel wall interacts with high affinity receptors expressed in several tissues including endothelium. Whether TXA(2) might alter endothelial migration tube formation, two determinants of angiogenesis, unknown. Thus, we investigated effect mimetic [1S-(1alpha, 2beta(5Z),3alpha(1E,3R), 4alpha]-7-[3-(3-hydroxy-4-(4'-iodophenoxy)-1-butenyl)-7-o xab icyclo- [2.2.1]heptan-2-yl]-5'-heptenoic acid (IBOP)...
The cyclin D1 gene encodes the regulatory subunit of a holoenzyme that phosphorylates and inactivates retinoblastoma protein, thereby promoting cell-cycle progression. Cyclin is overexpressed in hematopoetic epithelial malignancies correlating with poor prognosis metastasis several cancer types. Because tumor-associated macrophages have been shown to enhance malignant progression metastasis, D1-deficient mice are resistant oncogene-induced malignancies, we investigated function D1-/- bone...
Vascular endothelial functions, other than nitric oxide (NO)-mediated control of vasomotor tone, are poorly characterized in patients with chronic heart failure (CHF). Veins and arteries exposed to the same circulating proinflammatory mediators CHF. The present study tested whether cell activation occurs veins decompensated CHF activation, if present, subsides return a clinically compensated state.Fifteen requiring transient inotropic support 6 age-matched, healthy controls were studied....
Chagas' disease is caused by infection with the parasite Trypanosoma cruzi. We report that infected, but not uninfected, human endothelial cells (ECs) released thromboxane A2 (TXA2). Physical chromatography and liquid chromatography-tandem mass spectrometry revealed TXA2 predominant eicosanoid present in all life stages of T. Parasite-derived accounts for up to 90% circulating levels infected wild-type mice, perturbs host physiology. Mice which gene receptor (TP) has been deleted, exhibited...
Triple-negative breast cancer (BCa) (TNBC) is a deadly form of human BCa with limited treatment options and poor prognosis. In our prior analysis over 2200 samples, the G protein-coupled receptor CCR5 was expressed in > 95% TNBC samples. A humanized monoclonal antibody to (leronlimab), used HIV-infected patients, has shown minimal side effects large patient populations.A CCR5, leronlimab, for first time tissue culture mice determine binding characteristics cells, intracellular signaling,...
Ischemia-induced acidification of astrocytes or cardiac myocytes reduces intercellular communication by closing gap junction channels and subsequently internalizing proteins. To determine whether such coupling changes might be attributable to altered interactions between connexin43 (Cx43) other proteins, we applied the nigericin/high K+ method vary intracellular pH (pHi) in cultured cortical astrocytes. Intracellular was accompanied internalization Cx43 with retention scaffolding protein...
Inactivation of the transcription factor p53 is central to carcinogenesis. Yet only approximately one-half cancers have loss-of-function mutations. Here, we demonstrate a mechanism for inactivation by apoptosis repressor with caspase recruitment domain (ARC), protein induced in multiple cancer cells. The direct binding nucleus ARC tetramerization inhibits tetramerization. This exposes nuclear export signal p53, triggering Crm1-dependent relocation cytoplasm. Knockdown endogenous breast cells...
Vascular endothelial growth factor (VEGF) is an important patho-physiological mediator of angiogenesis. VEGF-induced cell (EC) migration and angiogenesis often occur in complicated environments containing multiple agents capable modifying the response. Thromboxane (TX) A2 released from types a prime pathogenesis many vascular diseases. Human EC express both TXA2 receptor (TP) isoforms; however, effects individual TP isoforms on angogenesis are unknown. We report here that mimetic...
Murine macrophages have been classified as either susceptible or nonsusceptible to killing by anthrax lethal toxin (LT) depending upon genetic background. While considered resistant LT killing, we found that bone marrow-derived (BMMs) from DBA/2, AKR, and C57BL/6 mice were slowly killed apoptosis following exposure. was not restricted in vitro assays, splenic also depleted LT-injected mice. Human macrophages, resistant, similarly underwent slow response challenge. In contrast, triggered...
Aldosterone (Aldo) activates both genomic and nongenomic signaling pathways in the cardiovascular system. Activation of contributes to adverse cardiac actions Aldo during reperfusion injury; however, extent contribute has been difficult identify due lack a specific ligand that only pathways. Using pegylated aldosterone analog, aldosterone-3-carboxymethoxylamine-TFP ester conjugated methoxypegylated amine (Aldo-PEG), we are able for first time distinguish between Aldo. We confirm Aldo-PEG...