A5058916400- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Cell death mechanisms and regulation
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Immune Response and Inflammation
- NF-κB Signaling Pathways
- interferon and immune responses
- Vector-borne infectious diseases
- CAR-T cell therapy research
- Toxin Mechanisms and Immunotoxins
- Systemic Lupus Erythematosus Research
- Viral Infections and Immunology Research
- Cytokine Signaling Pathways and Interactions
- Phagocytosis and Immune Regulation
- Endoplasmic Reticulum Stress and Disease
- Lymphoma Diagnosis and Treatment
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Signaling Pathways in Disease
- Inflammasome and immune disorders
- Gut microbiota and health
- Glycosylation and Glycoproteins Research
- Clostridium difficile and Clostridium perfringens research
- COVID-19 Clinical Research Studies
- Sulfur Compounds in Biology
University of Vermont
2014-2023
In-Q-Tel
2008
University of Alabama at Birmingham
2006
University of Lausanne
1996-2000
University of Vermont Medical Center
2000
IDEX Corporation (United States)
1996
Center for Rheumatology
1995
Stanford University
1988-1993
Pew Research Center
1991
Ludwig Cancer Research
1986-1990
The Pgp-1 glycoprotein was identified on a minor (27%) subset of peripheral Lyt-2+ or L3T4+ T cells. In contrast, mature medullary-type thymocytes (Lyt-2+ L3T4-, Lyt-2- L3T4+) were nearly devoid cells expressing detectable surface Pgp-1. appearance Pgp-1- found to be thymus dependent, as demonstrated by the diminished proportion after thymectomy and their virtual absence in athymic nude mice. subsequent acquisition stable differentiation event occurring concomitantly with primary antigenic...
Triggering of Fas (CD95) by its ligand (FasL) rapidly induces cell death via recruitment the adaptor protein Fas-associated domain (FADD), resulting in activation a caspase cascade. It was thus surprising that T lymphocytes deficient FADD were reported recently to be not only resistant FasL-mediated apoptosis, but also defective their proliferative capacity. This finding suggested potentially dual roles growth and for possibly other receptors. We report here CD3-induced proliferation...
Apoptotic death of T lymphocytes is critical for shutdown immune responses and hemopoietic cell homeostasis. Both receptor (Fas) activation mitochondrial apoptosis triggered by the BH3-only protein Bim have been implicated in killing antigen-stimulated cells. We examined mice lacking gene encoding (Bcl2l11) with inactivating lpr mutation Fas (Fas), designated Bcl2l11(-/-)Fas(lpr/lpr) mice. Shutdown an acute response to herpes simplex virus involved only no contribution Fas, whereas both...
A minor subset of immature (CD4-,8-) thymocytes that lack expression the B2A2 antigen was found to express low levels surface TCR protein as detected by mAbs F23.1 and KJ16 (reacting with products V beta 8 gene family). Interestingly, F23.1/KJ16 determinants were expressed on a two- three-fold higher proportion B2A2- than mature lymph node T cells in four independent haplotypes. When expanded short-term culture PMA calcium ionophore, retained their overexpression showed fivefold elevated...
Oxidative stress oligomerizes an outer mitochondrial membrane protein to trigger antiviral response in the absence of infection.
Fecal microbiota transplantation (FMT) is a promising new strategy in the treatment of Inflammatory Bowel Disease, but long-term delivery systems are lacking. This randomized study was designed as safety and feasibility FMT subjects with mild to moderate UC using frozen, encapsulated oral (cFMT).Subjects were 1:1 receive induction by colonoscopy, followed 12 weeks daily administration frozen cFMT or sham therpay. Subjects for 36 longitudenal clinical assessments included multiple subjective...
T lymphocytes with the surface phenotype CD4+8- and CD4-8+ are considered to be representative of functionally mature cells. We show here that adult murine thymus contains a subpopulation cells differ from found in periphery they do not express cell receptor-associated CD3 molecular complex. Such CD3-4-8+ thymocytes cortisone sensitive rapidly cycling situ. Furthermore, contrast cells, most low levels CD5 high B2A2 antigen. fail respond variety mitogenic stimuli vitro but give rise upon...
Abstract Fas is a cell surface molecule that expressed on wide array of types and triggers apoptosis. While in most situations ligation activates programmed death, resting T lymphocytes it can co‐stimulate proliferation with the receptor (TCR)/CD3 complex. This incongruity suggests may elicit signaling events overlap those used by cues. We observe human line Jurkat peripheral blood lymphocytes, stimulation does not signal Ras/Raf‐1/mitogen‐activated protein kinase (MAPK) pathway or increased...
The caspase 8 inhibitor c-FLIP(L) can act in vitro as a molecular switch between cell death and growth signals transmitted by the receptor Fas (CD95). To elucidate its function vivo, transgenic mice were generated that overexpress T-cell compartment (c-FLIP(L) Tg mice). As anticipated, FasL-induced apoptosis was inhibited T cells from mice. In contrast, activation-induced of unaffected, suggesting this deletion process proceed absence active 8. Accordingly, differed Fas-deficient showing no...
Reactive oxygen species (ROS) increase ligation of Fas (CD95), a receptor important for regulation programmed cell death. Glutathionylation reactive cysteines represents an oxidative modification that can be reversed by glutaredoxins (Grxs). The goal this study was to determine whether is redox regulated under physiological conditions. In study, we demonstrate stimulation with ligand (FasL) induces S-glutathionylation at cysteine 294 independently nicotinamide adenine dinucleotide phosphate...
Abstract Cellular FLIP long form (c-FLIPL) was originally identified as an inhibitor of Fas (CD95/Apo-1). Subsequently, additional functions c-FLIPL were through its association with receptor-interacting protein (RIP)1 and TNFR-associated factor 2 to activate NF-κB, well by activation caspase-8. T cells from c-FLIPL-transgenic (Tg) mice manifest hyperproliferation upon activation, although it not clear which the various involved. We have further explored effect on CD8+ effector cell function...
Murine peripheral Lyt-2+ T cells could be subdivided according to surface expression of the Pgp-1 glycoprotein into major (71%) Pgp-1- and minor (29%) Pgp-1+ subsets. A striking correlation was observed between enrichment for antigen-specific memory cytolytic lymphocyte precursors (CTLp). After immunization with male transplantation antigen H-Y, virtually all H-Y-specific CTLp were found in subset cells. In addition, after alloimmunization frequency allospecific resistant inhibition by...
In mtDNA mutator mice, mutations accumulate leading to a rapidly aging phenotype. However, there is little evidence of oxidative damage tissues, and when analyzed ex vivo, no change in production the reactive oxygen species (ROS) superoxide hydrogen peroxide by mitochondria has been reported, undermining mitochondrial theory aging. Paradoxically, interventions that decrease ROS levels vivo delay onset To reconcile these findings, we used mitochondria-targeted mass spectrometry probe MitoB...
Recent investigations in humans and mouse models with lupus have revealed evidence of mitochondrial dysfunction production reactive oxygen species (mROS) T cells neutrophils. This can provoke numerous cellular changes including oxidation nucleic acids, proteins, lipids even induction cell death. We previously observed that from patients lupus, the increased mROS is capable provoking oligomerisation antiviral stimulator (MAVS) type I interferon (IFN-I). SLE neutrophils also promotes formation...