A5067101223- Essential Oils and Antimicrobial Activity
- Sphingolipid Metabolism and Signaling
- Platelet Disorders and Treatments
- Ethnobotanical and Medicinal Plants Studies
- Cellular Mechanics and Interactions
- Blood properties and coagulation
- Cell Adhesion Molecules Research
- SARS-CoV-2 and COVID-19 Research
- Reproductive System and Pregnancy
- Computational Drug Discovery Methods
- Microbial bioremediation and biosurfactants
- Agricultural Economics and Practices
- Ovarian cancer diagnosis and treatment
- Biochemical and Structural Characterization
- Blood Coagulation and Thrombosis Mechanisms
- Antiplatelet Therapy and Cardiovascular Diseases
- Hepatitis C virus research
- Hippo pathway signaling and YAP/TAZ
- Antibiotics Pharmacokinetics and Efficacy
- Phytochemicals and Medicinal Plants
- Natural Antidiabetic Agents Studies
- Pregnancy and preeclampsia studies
- Microbial Community Ecology and Physiology
- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
Rajiv Gandhi Cancer Institute and Research Centre
2016-2025
Central University of Rajasthan
2014-2024
Indian Institute of Technology Kharagpur
2022-2024
Indian Institute of Technology Kanpur
2023
Jawaharlal Nehru University
2023
Aalto University
2022
Institute of Medical Sciences
2022
Bombay Hospital
2022
International Hospital of Bahrain
2022
Amity University
2015-2021
The accumulation of smooth muscle and endothelial cells is essential for remodeling repair injured blood vessel walls. Bone marrow–derived progenitor have been implicated in vascular remodeling; however, the mechanisms underlying their recruitment to site injury remain elusive. Here, using real-time vivo fluorescence microscopy, we show that platelets provide critical signal recruits CD34+ bone marrow c-Kit+ Sca-1+ Lin− sites injury. Correspondingly, specific inhibition platelet adhesion...
Recruitment of human CD34+ progenitor cells toward vascular lesions and differentiation into has been regarded as a critical initial step in atherosclerosis. Previously we found that adherent platelets represent potential mediators cell homing besides their role thrombus formation. On the other hand, foam formation represents key process atherosclerotic plaque To investigate whether are involved recruitment endothelial cells, examined interactions cells. Cocultivation experiments showed...
Lysophosphatidic acid (LPA) is a ligand for LPA(1-3) of the endothelial differentiation gene family G-protein-coupled receptors, and LPA(4-8) related to purinergic receptor. Because structure-activity relationship (SAR) GPR92/LPA(5) limited whether LPA its preferred endogenous has been questioned in literature, this study we applied combination computational experimental site-directed mutagenesis LPA(5) residues predicted interact with headgroup LPA. Four involved recognition were identified...
Lipid-rich atherosclerotic plaques are vulnerable, and their rupture can cause the formation of a platelet- fibrin-rich thrombus leading to myocardial infarction ischemic stroke. Although role plaque-based tissue factor as stimulator blood coagulation has been recognized, it is not known whether through direct activation platelets. We isolated lipid-rich atheromatous from 60 patients with carotid stenosis identified morphologically diverse collagen type I- III-positive structures in that...
Lysophosphatidic acid (LPA) is a platelet-activating component of mildly oxidized LDL (mox-LDL) and lipids isolated from human atherosclerotic plaques. Specific antagonists platelet LPA receptors could be useful inhibitors thrombus formation in patients with cardiovascular disease.Short-chain analogs phosphatidic (PA) were examined for their effect on two initial responses, shape change Ca2+ mobilization. Dioctylglycerol pyrophosphate [DGPP(8:0)] dioctylphosphatidic [PA(8:0)], recently...
Glycoprotein VI (GPVI) is the essential platelet collagen receptor in atherothrombosis, but its inhibition causes only a mild bleeding tendency. Thus, targeting this has selective antithrombotic potential. This study sought to compare compounds interfering with GPVI–atherosclerotic plaque interaction improve current antiatherothrombotic therapy. Human atherosclerotic plaque–induced aggregation was measured anticoagulated blood under static and arterial flow conditions (550/s, 1,100/s,...
Delhi has emerged as one of the greenest capital city world. Microbiological assessment drinking water emphasizes estimation hygienic quality sold with reference to community health significance. This study was conducted evaluate by roadside vendors in east, west, north and south zones India. A total number 36 samples (nine from each zone) were collected per national guidelines studied for microbiological assessment. All gamma-sterilized bottles kept an ice pack prevent any significant...
The dengue virus (DENV) infection commonly triggers threatening seasonal outbreaks all around the globe (estimated yearly infections are in order of 100 million, combining viral serotypes), testifying need for early detection to facilitate disease management and patient recovery. laboratory-based testing procedures detecting DENV enough challenged by resourced settings that result inevitable cost penalty unwarranted delay obtaining test results due distance-related factors with respect...
Context: Catharanthus roseus (periwinkle) is an important medicinal plant, mentioned in Ayurveda, ancient Indian Sanskrit literature. Th e plant selected to evaluate the possibility for novel pharmaceuticals since most of bacterial pathogens are developing resistance against currently available antibiotics. Aims: To determine antibacterial activity crude extracts from diff erent parts several species clinical signifi cance. Materials and Methods: Extraction each part appropriate solvent...
Various cytokines derived from placental cells are essential for normal placenta development and successful pregnancy. Interleukin-6 (IL-6) is a multifunctional cytokine produced by extravillous cytotrophoblasts regulating the functions of these cells, e.g. migration, invasion, trophoblast differentiation proliferation. In macrophages, newly synthesized IL-6 accumulates in Golgi complex exits tubulovesicular carriers fused with recycling endosomes secreted as soluble protein....
Abstract Gefitinib, an EGFR tyrosine kinase inhibitor, is used as FDA approved drug in breast cancer and non-small cell lung treatment. However, this has certain side effects complications for which the underlying molecular mechanisms are not well understood. By systems biology based silico analysis, we identified off-targets of gefitinib that might explain drugs. The crystal structure EGFR-gefitinib complex was binding pocket similarity searches on a druggable proteome database (Sc-PDB) by...
Dysregulation of protein kinases is associated with developmental defects and various human diseases. The kinome comprises 518 kinases, including several orphan whose functions remain to be fully characterized. NKF4 family, which includes STK35L1 PDIK1L, one such uncharacterized kinase family. STK35L1, also known as Clik1, was initially identified a nuclear actin fibers. Subsequent studies have demonstrated that plays critical roles in cellular processes cell cycle regulation, migration,...
Abstract The effect of replacing wheat flour with bran (0–10%), coarse (0–20%) or rice on the quality cookies was studied. cookie dough subjected to Instrumental Texture Profile Analysis in order determine cohesiveness and adhesiveness. After baking spread factor, puncture force fracture strength also determined. control had adhesiveness values 0.279 13.6 N s whereas 90.84 100.16 N. Wheat increased opposite effect. Increasing levels decreased but lowered factor where as factor. Coarse...
Cofilin is a regulator of actin filament dynamics. We studied whether during platelet activation Rho kinase stimulates LIM (LIMK) leading to subsequent phosphorylation and inactivation cofilin. Platelet shape change aggregation/secretion were induced by low high concentrations thrombin, respectively. found that these responses was responsible for mediating rapid Thr508 LIMK-1 the F-actin increase and, in part, secretion. Surprisingly, cofilin unaltered, first rapidly dephosphorylated an...
Lysophosphatidic acid (LPA), a component of mildly-oxidized LDL and the lipid rich core atherosclerotic plaques, elicits platelet activation. LPA is ligand G protein-coupled receptors (GPCR) EDG family (LPA1–3) newly identified LPA4–7 subcluster. LPA4, LPA5 LPA7 increase cellular cAMP levels that would induce inhibition rather than In present study we quantified mRNA LPA1–7 GPCR in human platelets found rank order LPA4 = > LPA6 LPA2 ≫ LPA1 LPA3. We examined shape change using panel receptor...
The medical world is on an immense requirement to discover novel antibiotics due widespread emergence of resistance among microbial pathogens against currently available antibiotics. Traditional plants have been proved be better source in the search for antimicrobial compounds. In such effort, we accessed susceptibilities some clinically significant bacterial species various extracts made up from leaves Ocimum sanctum L. (family: Lamiaceae). Antibacterial activity crude was found reliant...
Background ADF/cofilin proteins are key regulators of actin dynamics. Their function is inhibited by LIMK-mediated phosphorylation at Ser-3. Previous in vitro studies have shown that dependent on its concentration, cofilin either depolymerizes F-actin (at low concentrations) or promotes polymerization high concentrations). Methodology/Principal Findings We found after vivo cross-linking with different probes, a oligomer (65 kDa) could be detected platelets and endothelial cells. The did not...
Protegrin‐4 (PG‐4) is a member of the porcine leukocyte protegrins family cysteine‐rich antimicrobial peptides (AMPs) isolated from Sus scrofa . It consists 18 amino acid residues and works as part innate immune system. In this study, we examined intrinsic aggregation propensity AMP using multiple computational algorithms, namely, TANGO, AGGRESCAN, FOLDAMYLOID, AMYLPRED, ZYGGREGATOR, found that peptide predicted to have high for β sheet formation disposes be amyloidogenic. Under in vitro...
Background Colorectal cancer (CRC) is a heterogeneous disease morphologically, histologically, and molecularly. Most of the studies are on this molecular heterogeneity their clinicopathological correlation from western world. Very few have been done in India. Objectives The aims study were to evaluate clinical pathological profile CRCs, determine frequency subtypes correlate between features, association different CRC. Materials Methods A prospective noninvasive interventional was 50...