A5009178208- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- T-cell and B-cell Immunology
- Cancer Immunotherapy and Biomarkers
- Monoclonal and Polyclonal Antibodies Research
- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- vaccines and immunoinformatics approaches
- Immune Response and Inflammation
- Hematopoietic Stem Cell Transplantation
- Systemic Lupus Erythematosus Research
- Diabetes and associated disorders
- Cytomegalovirus and herpesvirus research
- Chronic Lymphocytic Leukemia Research
- Immunodeficiency and Autoimmune Disorders
- Reproductive System and Pregnancy
- Asthma and respiratory diseases
- Cancer Research and Treatments
- Viral-associated cancers and disorders
- Glycosylation and Glycoproteins Research
- Cancer Genomics and Diagnostics
- Toxin Mechanisms and Immunotoxins
- Infant Nutrition and Health
- Lymphoma Diagnosis and Treatment
Medigene (Germany)
2015-2025
Helmholtz Zentrum München
2008-2017
Institute of Bioinformatics and Systems Biology
2015
Technical University of Munich
2015
Center for Integrated Protein Science Munich
2014
Ludwig-Maximilians-Universität München
1993-2014
University of Fribourg
2014
University Hospital Cologne
2014
Roche (Switzerland)
2014
Immune Regulation (United Kingdom)
2012
Nine cancer patients were treated with adoptive cell therapy using autologous anti-MAGE-A3 T-cell receptors (TCR)-engineered T cells. Five experienced clinical regression of their cancers including 2 on-going responders. Beginning 1–2 days postinfusion, 3 (#'s 5, 7, and 8) mental status changes, (5 lapsed into comas subsequently died. Magnetic resonance imagining analysis 5 8 demonstrated periventricular leukomalacia, examination brains at autopsy revealed necrotizing leukoencephalopathy...
The cell-mediated lympholytic capability of mouse spleen cells stimulated in mixed lymphocyte culture is related to the major histocompatibility complex genotype on target lymphocytes. strain combinations AQR-B10. T(6R) and B10.A(4R)-B10.A(2R) that result significant activation do not mediate lympholysis sensitizing lymphocytes; serologically defined regions (H-2K H-2D) are identical within each combination. H-2K or H-2D region disparity alone does cause lympholysis. However after as seen...
In many solid tumors, cancer stem cells (CSC) represent a population with tumor-initiating, self-renewal, and differentiation potential, which can be identified by surface protein markers. No generally applicable markers are yet known for renal cell carcinoma (RCC). Two RCC lines (RCC-26, RCC-53) were found to differ widely in their capacity form spheres vitro establish tumors mice, potentially reflecting differences CSC content. A subpopulation expressing the CXC chemokine receptor 4...
Abstract Metastatic renal cell carcinoma (RCC), like melanoma, belongs to the small group of human tumors in which partial or complete remission has been observed some patients after treatment with various forms immunotherapy. In contrast CTL showing MHC-restricted lysis RCC have not easily found among tumor-infiltrating lymphocytes (TIL). This led suggestion by that responses immunotherapy are mediated predominantly non-MHC-restricted effector cells. We characterized an MHC-restricted, CD8+...
Glutamic acid decarboxylase (GAD) has been defined as a major target antigen in insulin-dependent diabetes mellitus (IDDM). To identify the molecular ligands triggering T cell response to GAD, panel of human GAD65-specific lymphocyte lines was generated from peripheral blood three recent onset IDDM patients. All derived patient expressing high-risk-conferring HLA-DR*0301/ *0401 haplotypes recognized single epitope localized between amino positions 270 and 283 GAD65, stretch that is located...
In a multicenter study more than 300 central European systemic lupus erythematosus (SLE) patients were examined for HLA-B, HLA-DR, and complement C4 phenotypes. For 174 SLE MHC haplotypes determined by family segregation analysis, 155 gene deletions TaqI restriction fragment length polymorphism. Two haplotypes, B8-C4AQ0-C4B1-DR3 B7-C4A3-C4B1-DR2, identified as risk factors SLE. These findings confirmed applying the haplotype frequency difference (HFD) method, which uses nontransmitted from...
Abstract Purpose: Renal cell carcinoma harbors high numbers of infiltrating lymphocytes with apparent limited efficacy in tumor control. This study focused on the natural killer (NK) cells renal carcinoma. Experimental Design: Tumor-infiltrating (TIL) were isolated from and analyzed for NK frequency phenotype (n = 34). enriched tested effector function. Results: Two subtypes identified, one containing (>20% lymphocyte population, n 14), other low (<20%, 20), numbers. both...
Abstract Psoriasis vulgaris is an inflammatory skin disease characterized by excessively increased keratinocyte proliferation. Several lines of evidence support the idea that T cells infiltrating psoriatic lesions play a vital role in pathogenesis disease. To establish whether lesional accumulation and activation lymphocytes reflect specific local immune response, TCR beta-chain variable (V beta) region gene usage was studied chronic plaques, normal skin, paired blood lymphocytes. By...
Both cytokines produced by activated monocytes and T cells direct cell-to-cell contact with antigen-primed during inflammatory reactions are known to induce the expression of several adhesion proteins on endothelial cells. In this prospective longitudinal study, we analyzed ELAM-1, VCAM-1, ICAM-1 myocardial allograft biopsy specimens taken from 16 cardiac recipients either for routine monitoring or investigation suspected rejection. Infiltrating were identified using anti-CD3 antibodies....
Abstract Background For optimal T cell activation it is desirable that dendritic cells (DCs) display peptides within MHC molecules as signal 1, costimulatory 2 and, in addition, produce IL-12p70 3. polarizes responses towards CD4 + helper 1 cells, which then support the development of CD8 cytotoxic lymphocytes. We therefore developed new maturation cocktails allowing DCs to biologically active for large-scale cancer vaccine development. Methods After elutriation leukapheresis products a...
The consensus motifs of HLA-Cw3, -Cw4, -Cw6, and -Cw7 ligands were determined by pool sequencing. Together with information obtained sequencing some prominent individual peptides, the results indicate following: (i) all four HLA-C molecules are associated peptides. (ii) These peptides adhere to allele-specific that similar those HLA-A or -B molecules; they have a preferred length nine amino acids an anchor residue at C terminus. (iii) All analyzed exhibit related peptide motifs, although...
In a prospective study the immunogenicity of Zwa antigen during gestation was investigated. Twenty‐six out 1,211 pregnant women were negative (2.15%). 2 23 mothers who delivered positive children, an antibody to detected. To determine relationship between immune response towards and MHC, phenotype frequencies HLA‐A, B, C, DR, complement BF, C2, C4A, C4B allotypes “non‐re sponders” without detectable antibodies (N=20) compared with group “responders”, i.e. giving birth children typical...
Abstract BACKGROUND Prostate cancer is the most common of men in Western world. Despite over‐expression tumor‐associated antigens, like PSA or PSMA, immune activation inefficient. The goal this investigation was to assess situ characteristics prostate cancer‐infiltrating lymphocytes and determine their status effector function. METHODS We compared 17 carcinoma containing tissues, four benign prostatic hyperplasia tissues eight healthy regarding lymphocyte subset composition, locoregional...