Abstract Purpose: The inflammatory enzyme indoleamine 2,3-dioxygenase (IDO) participates in immune tolerance and tumor escape processes by degradation of the essential amino acid tryptophan formation toxic catabolites. Here, we analyzed role IDO growth disease progression patients with clear cell renal carcinoma (RCC). Experimental Design: Expression mRNA was quantitative reverse transcription-PCR 55 primary 52 metastatic RCC, along 32 normal kidneys. Western blot immunohistochemistry...

Abstract Purpose: Renal cell carcinoma harbors high numbers of infiltrating lymphocytes with apparent limited efficacy in tumor control. This study focused on the natural killer (NK) cells renal carcinoma. Experimental Design: Tumor-infiltrating (TIL) were isolated from and analyzed for NK frequency phenotype (n = 34). enriched tested effector function. Results: Two subtypes identified, one containing (>20% lymphocyte population, n 14), other low (<20%, 20), numbers. both...

The biliary glycoproteins (BGP or CD66a), a group of different splice variants single gene, are members the carcinoembryonic antigen family and immunoglobulin superfamily. Recently, we detected CD66a on IL-2 activated lymphocytes. In this study characterized structure expression pattern BGP human lymphocytes investigated its role in T cell activation. Lymphocytes express 2 13 known variants, i.e. BGPa BGPb, which glycosylated lymphocyte-specific manner. Both BGPb have long cytoplasmic tail,...

Most rapidly evolving gene families are involved in immune responses and reproduction, two biological functions which have been assigned to the carcinoembryonic antigen (CEA) family. To gain insights into evolutionary forces shaping CEA family we analysed this 27 mammalian species including monotreme marsupial lineages. Phylogenetic analysis provided convincing evidence that primordial mammals consisted of five genes, inhibitory receptor-encoding CEACAM1 (CEA-related cell adhesion molecule)...

CEACAM1, CEA/CEACAM5, and CEACAM6 are cell adhesion molecules (CAMs) of the carcinoembryonic antigen (CEA) family that have been shown to be deregulated in lung cancer up 50% all human cancers. However, little is known about functional impact these on undifferentiated growth tumor progression. Here we demonstrate surface expression CEACAM1 confluent A549 adenocarcinoma cells plays a critical role differentiated, contact-inhibited growth. Interestingly, CEACAM1-L, but not CEACAM1-S,...

Abstract Carcinoembryonic Ag-related cell adhesion molecule 1 (CEACAM1), the primordial carcinoembryonic Ag gene family member, is a transmembrane expressed in leukocytes, epithelia, and blood vessel endothelia humans rodents. As result of differential splicing, CEACAM1 occurs as several isoforms, two major ones being CEACAM1-L CEACAM1-S, that have long (L) or short (S) cytoplasmic domains, respectively. The L:S expression ratios vary different cells tissues. In addition to CEACAM1, human...

Abstract Background Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1), an immunoglobulin (Ig)-related glycoprotein, serves as cellular receptor for a variety of Gram-negative bacterial pathogens associated with the human mucosa. In particular, Neisseria gonorrhoeae , N. meningitidis Moraxella catarrhalis and Haemophilus influenzae possess well-characterized CEACAM1-binding adhesins. CEACAM1 is typically involved in cell-cell attachment, epithelial differentiation,...

Abstract Dendritic cells (DC) are important APCs that play a key role in the induction of an immune response. The signaling molecules govern early events DC activation not well understood. We therefore investigated whether express carcinoembryonic Ag-related cell adhesion molecule 1 (CEACAM1, also known as BGP or CD66a), well-characterized signal-regulating cell-cell is expressed on granulocytes, monocytes, and activated T B cells. found murine vitro vivo both major isoforms CEACAM1,...

Granulocytes form the first and fastest line of defense against pathogenic infections. Their survival is limited by apoptosis, a process that critical for resolution inflammation. Pro-apoptotic pro-inflammatory cytokines, as well several receptors, can alter lifespan granulocytes. Here we report carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1, CD66a) involved in regulation granulocyte survival. Until now CEACAM1 described to control proliferation, migration, tumor growth,...

Human T and natural-killer (NK) cells, that are thought to be the major cytotoxic effector-cell populations in defence against neoplastic were isolated from blood decidua order analyze their expression of carcinoembronic-antigen-(CEA)-family-member proteins. Biliary glycoprotein (BGP, CD66a) was only member carcinoembryonic antigen family detected. While freshly T-cells expressed low amounts BGP, NK cells negative. After vitro stimulation for 3 days,T up-regulated BGP a sub-group (CD16−...

Carcinoembryonic antigen cell adhesion molecule-1 (CEACAM1) is an immunoglobulin-like surface co-receptor expressed on epithelial, hematopoietic and endothelial cells. CEACAM1 functions as molecule, mainly binding to itself or other members of the CEA family. We others have previously shown that crucial for in vivo vascular integrity during ischemic neo-vascularization. Here, we deciphered roles normal pathological vascularization. found Ceacam1−/− mice exhibit a significant increase basal...

Background Photodynamic therapy (PDT) uses the combination of photosensitizing drugs and harmless light to cause selective damage tumor cells. PDT is therefore an option for focal localized disease or otherwise unresectable tumors. In addition, there increasing evidence that can induce systemic anti-tumor immunity, supporting control cells, which were not eliminated by primary treatment. However, effect non-lethal on behavior malignant potential cells surviving molecularly well defined....

The vertebrate-restricted carcinoembryonic antigen gene family evolves extremely rapidly. Among their widely expressed members, the mammal-specific, secreted CEACAM16 is exceptionally well conserved and specifically in inner ear. To elucidate a potential auditory function, we inactivated murine Ceacam16 by homologous recombination. In young Ceacam16−/− mice hearing threshold for frequencies below 10 kHz above 22 was raised. This impairment progressed with age. A similar phenotype observed...

Non-invasive sampling techniques based on the analysis of oral fluid specimen have gained substantial importance in field swine herd management. Methodological advances a focus endemic viral diseases commercial pig production. More recently, these approaches been adapted to non-invasive wild boar for transboundary animal disease detection which effective population level methods not available. In this study, rope-in-a-bait technique was tested detect classical fever virus nucleic acid...

Abstract Background Pregnancy-specific glycoprotein ( PSG ) genes belong to the carcinoembryonic antigen CEA gene family, within immunoglobulin superfamily. In humans, 10 encode closely related secreted glycoproteins. They are exclusively expressed in fetal syncytiotrophoblast cells and represent most abundant proteins maternal blood. recent years, a role modulation of immune system possibly avoid rejection semiallogeneic fetus facilitate access trophoblast resources via blood has been...

Cytoplasmic aggregation of the primarily nuclear TAR DNA-binding protein 43 (TDP-43) affects neurons in most amyotrophic lateral sclerosis (ALS) and approximately half frontotemporal lobar degeneration (FTLD) cases. The cellular prion protein, PrPC, has been recognized as a common receptor downstream effector circulating neurotoxic species several proteins involved neurodegeneration. Here, capitalizing on our recently adapted TDP-43 real time quaking induced reaction, we set reproducible...

Abstract A viral oncogene carrying well-defined Kb/Db-restricted epitopes was expressed in a heat shock protein (hsp)-associated or nonassociated form the murine tumor cells P815 and Meth-A. Wild-type SV40 large T-Ag (wtT-Ag) is without stable hsp association; mutant (cytoplasmic cT-Ag) chimeric (cT272-green fluorescent fusion protein) association with constitutively expressed, cytosolic hsp73 (hsc70) protein. In vitro, remnants from apoptotic wtT-Ag- cT-Ag-expressing are taken up processed...

Abstract CEACAM1 acts as a tumour suppressor in various epithelial tumours. On the other hand, de novo expression of is strongly associated with reduced disease‐free survival melanoma and non‐small cell lung carcinoma patients. Since effector functions natural killer T cells are inhibited by homophilic interaction, immune escape could be responsible for poor prognosis these Here, we describe normal kidney, renal adenomas carcinomas (RCC) using novel antibody generated genetic immunization....

Abstract The multifunctional carcinoembryonic Ag cell adhesion molecule (CEACAM)1 protein has recently become the focus of intense immunological research. We have previously shown that CEACAM1 homophilic interactions inhibit killing activity NK cells. This novel inhibitory mechanism plays a key role in melanoma immune evasion, inhibition decidual response, and controlling autoreactivity TAP2-deficient patients. These roles are mediated mainly by interactions, which through N-domain CEACAM1....