A5090277402- Viral-associated cancers and disorders
- Cytomegalovirus and herpesvirus research
- Lymphoma Diagnosis and Treatment
- Immune Cell Function and Interaction
- Parvovirus B19 Infection Studies
- Herpesvirus Infections and Treatments
- Histiocytic Disorders and Treatments
- Virus-based gene therapy research
- Polyomavirus and related diseases
- Extracellular vesicles in disease
- Eosinophilic Disorders and Syndromes
- DNA Repair Mechanisms
- CAR-T cell therapy research
- Genomics and Chromatin Dynamics
- interferon and immune responses
- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- SARS-CoV-2 and COVID-19 Research
- Circular RNAs in diseases
- T-cell and Retrovirus Studies
- CRISPR and Genetic Engineering
- Cancer-related gene regulation
- SARS-CoV-2 detection and testing
- HIV Research and Treatment
- COVID-19 Clinical Research Studies
German Center for Infection Research
2016-2025
Helmholtz Zentrum München
2016-2025
Center for Environmental Health
1993-2021
Matrix Research (United States)
2019
Ludwig-Maximilians-Universität München
1997-2013
Institute for Environment and Human Security
2001-2008
National Center for Tumor Diseases
2005
University of Birmingham
2002-2003
Grieg Seafood (Norway)
2003
Cancer Research UK
2002
With current techniques, genetic alterations of herpesviruses are difficult to perform, mostly because the large size their genomes. To solve this problem, we have designed a system that allows cloning any γ-herpesvirus in Escherichia coli onto an F factor-derived plasmid. Immortalized B cell lines were readily established with recombinant Epstein–Barr virus (EBV), demonstrating factor-cloned EBV genome has all characteristics wild-type EBV. Because modification is possible E. , experimental...
A strategy for cloning and mutagenesis of an infectious herpesvirus genome is described. The mouse cytomegalovirus was cloned maintained as a 230 kb bacterial artificial chromosome (BAC) in E. coli . Transfection the BAC plasmid into eukaryotic cells led to productive virus infection. feasibility introduce targeted mutations shown by mutation immediate-early 1 gene generation mutant virus. Thus, complete construction can now be carried out controlled manner prior reconstitution progeny....
Although microRNA (miRNA) regulation of TLR signaling is well established, this has not yet been observed for NLR proteins or the inflammasomes they form. We have now validated a highly conserved miR-223 target site in NLRP3 3'-untranslated region. expression decreases as monocytes differentiate into macrophages, whereas protein increases during time. However, overexpression prevents accumulation and inhibits IL-1β production from inflammasome. Virus inhibition inflammasome an emerging...
Epstein-Barr virus (EBV) is a human tumor and model of herpesviral latency. The efficiently infects resting B lymphocytes induces their continuous proliferation in vitro, which mimics certain aspects EBV's oncogenic potential vivo. How lymphoblastoid cell lines (LCLs) evolve from the infected uncertain. We conducted systematic time-resolved longitudinal study cellular functions transcriptional profiles newly naïve primary lymphocytes. EBV reprograms cells comprehensively globally. Rapid...
Mammalian cells release different types of vesicles, collectively termed extracellular vesicles (EVs). EVs contain cellular microRNAs (miRNAs) with an apparent potential to deliver their miRNA cargo recipient affect the stability individual mRNAs and cells' transcriptome. The extent which miRNAs are exported via EV route whether they contribute cell-cell communication controversial. To address these issues, we defined multiple properties analyzed capacity packaged into target exert...
DNA viruses such as herpesviruses are known to encode homologs of cellular antiapoptotic viral Bcl-2 proteins (vBcl-2s), which protect the virus from apoptosis in its host cell during synthesis. Epstein-Barr (EBV), a human tumor and prominent member γ-herpesviruses, infects primary resting B lymphocytes establish latent infection yield proliferating, growth-transformed cells vitro. In these cells, 11 genes that contribute transformation consistently expressed. EBV also encodes two vBcl-2...
Cellular and viral microRNAs (miRNAs) are involved in many different processes of key importance more than 10,000 miRNAs have been identified so far. In general, relatively little is known about their biological functions mammalian cells because phenotypic effects often mild targets still await identification. The recent discovery that Epstein-Barr virus (EBV) other herpesviruses produce own, barely conserved sets suggests these viruses usurp the host RNA silencing machinery to advantage...
The Epstein–Barr virus (EBV) nuclear antigen 1 (EBNA1) is one of the earliest viral proteins expressed after infection and only latent protein consistently in viral-associated tumors. EBNA1's crucial role DNA replication, episomal maintenance, partitioning well examined whereas its importance for immortalization process tumorgenicity EBV unclear. To address these open questions, we generated, based on maxi-EBV system, an EBNA1-deficient mutant used this strain to infect primary human B...
EBV, a member of the herpes virus family, is paradigm for human tumor viruses and model viral latency amenable study in vitro. It induces resting B lymphocytes to proliferate indefinitely vitro initially establishes strictly latent infection these cells. BZLF1 , related cellular activating protein 1 (AP-1) family transcription factors, master gene essential sufficient mediate switch induce EBV lytic phase latently infected Enigmatically, after expressed very early majority primary cells, but...
Lifelong persistence of Epstein-Barr virus (EBV) in infected hosts is mainly owed to the virus' pronounced abilities evade immune responses its human host. Active evasion mechanisms reduce immunogenicity cells and are known be major importance during lytic infection. The EBV genes BCRF1 BNLF2a encode viral homologue IL-10 (vIL-10) an inhibitor transporter associated with antigen processing (TAP), respectively. Both immunoevasins EBV's phase. Here we describe that functionally expressed...
A previously unrecognized activity has been associated with the product of BNLF-1 gene Epstein-Barr virus. This encodes latent membrane protein When was expressed at high levels, it toxic to all cell lines tested, which included six human B-lymphoid as well BALB/3T3, 143/EBNA-1, and HEp-2 cells. The shown induce anchorage-independent tumorigenic growth in Rat-1 BALB/3T3 We demonstrate here that only those mutations score positively assay were cytotoxic when levels. It is therefore possible...
Significance Most humans are infected for their lifetime with Epstein–Barr virus (EBV), which can cause cancer and other EBV-associated diseases. Infected individuals develop strong immune responses to this virus, in particular cytotoxic CD8 + T cells, but viral infection is never cleared nor EBV eliminated from the body. This suggests that certain molecules might prevent effective elimination of EBV-infected cells by cells. rich genes coding microRNAs, many unknown function. We show...
Epstein-Barr virus (EBV) is a tumor that establishes lifelong infection in most of humanity, despite eliciting strong and stable virus-specific immune responses. EBV encodes at least 44 miRNAs, them with unknown function. Here, we show multiple miRNAs modulate recognition recently infected primary B cells, EBV's natural target cells. collectively specifically suppress release proinflammatory cytokines such as IL-12, repress differentiation naive CD4+ T cells to Th1 interfere peptide...
Epstein-Barr virus (EBV) infects and activates resting human B lymphocytes, reprograms them, induces their proliferation, establishes a latent infection in them. In established EBV-infected cell lines, many viral genes are expressed. Their roles supporting the continuous proliferation of cells