A5071327421- Ion channel regulation and function
- Cardiac electrophysiology and arrhythmias
- Neuroscience and Neural Engineering
- Ion Channels and Receptors
- Neuroscience and Neuropharmacology Research
- Cardiomyopathy and Myosin Studies
- Advanced Fluorescence Microscopy Techniques
- Protein Kinase Regulation and GTPase Signaling
- Receptor Mechanisms and Signaling
- Cellular transport and secretion
- History and advancements in chemistry
- Calcium signaling and nucleotide metabolism
- CRISPR and Genetic Engineering
- Erythrocyte Function and Pathophysiology
- Mitochondrial Function and Pathology
- Inorganic and Organometallic Chemistry
- Analytical Chemistry and Sensors
- Cell Image Analysis Techniques
- Cloud Data Security Solutions
- Pluripotent Stem Cells Research
- Health and Medical Studies
- Blood properties and coagulation
- Analytical Chemistry and Chromatography
- Cellular Mechanics and Interactions
- ATP Synthase and ATPases Research
Saarland University
2016-2025
Humboldt-Universität zu Berlin
2022
Charité - Universitätsmedizin Berlin
2022
Institut für Informationsverarbeitung
2014-2019
Graz University of Technology
1992-2015
Universitätsklinikum des Saarlandes
2011-2015
German Centre for Cardiovascular Research
2014
Heidelberg University
2014
Cornell University
2014
Faculty (United Kingdom)
2012
Abstract Coordinated release of calcium (Ca 2+ ) from the sarcoplasmic reticulum (SR) through cardiac ryanodine receptor (RYR2) channels is essential for cardiomyocyte function. In catecholaminergic polymorphic ventricular tachycardia (CPVT), an inherited disease characterized by stress‐induced arrhythmias in young patients with structurally normal hearts, autosomal dominant mutations RYR2 or recessive calsequestrin lead to aberrant diastolic Ca SR causing arrhythmogenic delayed after...
Abstract The epicardium, the mesothelial envelope of vertebrate heart, is source multiple cardiac cell lineages during embryonic development and provides signals that are essential to myocardial growth repair. Here we generate self-organizing human pluripotent stem cell-derived epicardioids display retinoic acid-dependent morphological, molecular functional patterning epicardium myocardium typical left ventricular wall. By combining lineage tracing, single-cell transcriptomics chromatin...
1. Confocal microscopy was used to investigate hormone‐induced subcellular Ca2+ release signals from the endoplasmic reticulum (ER) in a prototype non‐excitable cell line (HeLa cells). 2. Histamine application evoked two types of elementary signals: (i) blips arising single ER channels (amplitude, 30 nM; lateral spreading, 1.3 microns); (ii) puffs resulting concerted activation several 170 4 microns). 3. waves HeLa cells arose variable number initiation sites, but for individual cells, and...
Various cardio‐active stimuli, including endothelin‐1 (ET‐1), exhibit potent arrhythmogenicity, but the underlying cellular mechanisms of their actions are largely unclear. We used isolated rat atrial myocytes and related changes in subcellular Ca 2+ signalling to ability various stimuli induce diastolic, premature extra transients (ECTs). For this, we recorded global spatially resolved signals indo‐1‐ fluo‐4‐loaded during electrical pacing. ET‐1 exhibited a higher arrhythmogenicity...
1. Na+ current (INa)‐induced Ca2+ transients were studied in ventricular myocytes isolated from adult guinea‐pig hearts. The fluorescent indicator fluo‐3 or a mixture of and fura‐red used conjunction with confocal microscopy to follow the intracellular concentration while membrane currents measured simultaneously whole‐cell configuration patch‐clamp technique. 2. release sarcoplasmic reticulum (SR) could be triggered either by (ICa) (INa). Analysis INa‐induced signals at higher temporal...
1. Subcellularly localized Ca2+ signals have been proposed to represent elementary events of cardiac signalling (Ca2+ sparks), whereby an individual sarcolemmal L‐type channel locally controls opening a single (or few) release channels in the sarcoplasmic reticulum (SR). 2. To investigate directly nature this Ca(2+)‐induced mechanism we used flash photolysis caged while simultaneously measuring intracellular concentration ([Ca2+]i) with laser‐scanning confocal microscope. 3. Power spectral...
Hyperaldosteronism is associated with an increased prevalence of atrial fibrillation (AF). However, it unclear whether this the consequence altered haemodynamics or a direct aldosterone effect. It was aim study to demonstrate load-independent effects on structure and electrophysiology.Osmotic mini-pumps delivering 1.5 µg/h were implanted subcutaneously in rats (Aldo). Rats without treatment served as controls. After 8 weeks, surface electrocardiogram, inducibility AF, pressures recorded...
Abstract Purpose The aim of this study was to compare the proliferation and attachment behavior fibroblasts epithelial cells on differently structured abutment materials. Materials Methods Three different surface topographies were prepared zirconia titanium alloy specimens defined as follows: machined (as delivered without further modification), smooth (polished), rough (sandblasted). Energy‐dispersive X ‐ray spectroscopy, topographical analysis, water contact angle measurements used analyze...
AimsPathological cardiac hypertrophy is a major predictor for the development of diseases. It associated with chronic neurohumoral stimulation and altered Ca2+ signalling in cardiomyocytes. TRPC proteins form agonist-induced cation channels, but their functional role homeostasis cardiomyocytes during fast cytosolic cycling leading to unknown.
Abstract Aims Inflammation plays an important role in cardiovascular disease (CVD) development. The NOD-like receptor protein-3 (NLRP3) inflammasome contributes to the development of atherosclerosis animal models. Components NLRP3 pathway such as interleukin-1β can therapeutically be targeted. Associations genetically determined inflammasome-mediated systemic inflammation with CVD and mortality humans are unknown. Methods results We explored association genetic variants prevalent 538 167...
Background: Cardiovascular diseases and chronic kidney disease (CKD) are highly prevalent, aggravate each other, account for substantial mortality. Both conditions characterized by activation of the innate immune system. The alarmin interleukin-1α (IL-1α) is expressed in a variety cell types promoting (sterile) systemic inflammation. aim present study was to examine role IL-1α mediating inflammation setting acute myocardial infarction (AMI) CKD. Methods: We assessed expression on surface...
Constant exposure of the airways to inhaled pathogens requires efficient early immune responses protecting against infections. How bacteria on epithelial surface are detected and first-line protective mechanisms initiated not well understood. We have recently shown that tracheal brush cells (BCs) express functional taste receptors. Here we report bitter signaling in murine BCs induces neurogenic inflammation. demonstrate BC stimulates adjacent sensory nerve endings trachea release...