A5009616566- Pediatric health and respiratory diseases
- Neonatal Respiratory Health Research
- Asthma and respiratory diseases
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Antimicrobial Peptides and Activities
- Immune Response and Inflammation
- IL-33, ST2, and ILC Pathways
- Psoriasis: Treatment and Pathogenesis
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Inhalation and Respiratory Drug Delivery
- Pneumonia and Respiratory Infections
- Cytokine Signaling Pathways and Interactions
- Respiratory Support and Mechanisms
- Immune cells in cancer
- Cancer Immunotherapy and Biomarkers
- Medical Imaging and Pathology Studies
- Antifungal resistance and susceptibility
- Vitamin D Research Studies
- Epigenetics and DNA Methylation
- Bacterial biofilms and quorum sensing
- Cystic Fibrosis Research Advances
- Congenital Diaphragmatic Hernia Studies
- COVID-19 Clinical Research Studies
- Antimicrobial Resistance in Staphylococcus
- SARS-CoV-2 and COVID-19 Research
Saarland University
2016-2025
Universitätsklinikum des Saarlandes
2011-2019
PHV Dialysezentrum
2018
Wilhelm Sander Stiftung
2017
University Medical Center
2014-2016
University Hospital and Clinics
2014
Philipps University of Marburg
2004-2009
University of Pennsylvania
2007-2009
Phillips University
2007
Universitätsklinikum Gießen und Marburg
2007
Antimicrobial peptides are endogenous antibiotics that directly inactivate microorganisms and in addition have a variety of receptor-mediated functions. LL-37/hCAP-18 is the only cathelicidin found humans involved angiogenesis regulation innate immune system. The aim present study was to characterize role peptide LL-37 wound closure airway epithelium cell line NCI-H292 primary epithelial cells. stimulated healing mechanically induced wounds monolayers differentiated epithelium. This effect...
Smoking increases the susceptibility to pulmonary infection and is a risk factor for development of chronic obstructive disease (COPD). It postulated that cigarette smoke suppresses activation innate immune system in response bacterial infection.Using sensitive ex vivo analysis, level endogenous antibiotic peptide human beta-defensin-2 (hBD-2) was measured pharyngeal washing fluid sputum from patients with community acquired pneumonia. The regulation antibacterial host defence molecules...
Constant exposure of the airways to inhaled pathogens requires efficient early immune responses protecting against infections. How bacteria on epithelial surface are detected and first-line protective mechanisms initiated not well understood. We have recently shown that tracheal brush cells (BCs) express functional taste receptors. Here we report bitter signaling in murine BCs induces neurogenic inflammation. demonstrate BC stimulates adjacent sensory nerve endings trachea release...
Epithelial cells represent the initial site of bacterial colonization in respiratory tract. TLR9 has been identified B and CD 123(+) dendritic found to be involved recognition microbial DNA. It was aim study investigate role host defense reactions epithelium. Respiratory epithelial cell lines (IHAEo(-), Calu-3) or fully differentiated primary human as air-liquid interface cultures were stimulated with DNA synthetic oligonucleotides containing CpG motifs (CpG oligodeoxynucleotides)....
Abstract The innate immune system of the lung is a multicomponent host defense and in addition has an instructing role regulating quality quantity adaptive response. When interaction between immunity disturbed, pathological conditions such as asthma can develop. It was aim study to investigate effect allergic inflammation on during bacterial infection. Human bronchial epithelial cells were preincubated with Th2 cytokines infected Pseudomonas aeruginosa. mRNA levels antimicrobial peptides...
Abstract Bacterial pathogens are a leading cause of lung infections and contribute to acute exacerbations in patients with chronic respiratory diseases. The innate immune system the tract controls prevents colonization bacterial pathogens. Forkhead box transcription factor family O (FOXO) factors key regulators cellular metabolism, proliferation, stress resistance. In this study, our aim was investigate role FOXO functions epithelial cells. We show that potently activate cultured human cells...
Alzheimer’s disease (AD) is characterized by extracellular plaques in the brain, mainly consisting of amyloid-β (Aβ), as derived from sequential cleavage amyloid precursor protein. Epidemiological studies suggest a tight link between hypovitaminosis secosteroid vitamin D and AD. Besides decreased level AD patients, an effect on Aβ-homeostasis discussed. However, exact underlying mechanisms remain to be elucidated nothing known about potential analogues. Here we systematically investigate...
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Cigarette smoke (CS) exposure is the predominant risk factor for development of chronic obstructive pulmonary disease (COPD) and third leading cause death worldwide. We aimed to elucidate whether mitochondrial respiratory inhibition oxidative stress are triggers in its etiology. In different models CS exposure, we investigated effect on lung remodeling cell signaling restoring electron flow using alternative oxidase (AOX), which bypasses cytochrome segment chain. AOX attenuated CS-induced...
Abstract Background The use of electronic cigarettes (ECIGs) is increasing, but the impact ECIG-vapor on cellular processes like inflammation or host defense are less understood. aim present study was to compare acute effects traditional (TCIGs) and ECIG-exposure defense, inflammation, activation cell lines primary differentiated human airway epithelial cells (pHBE). Methods We exposed pHBEs several TCIG-smoke ECIG-vapor. Epithelial barrier integrity were determined. transcriptome compared...
COVID-19 comprises several severity stages ranging from oligosymptomatic disease to multi-organ failure and fatal outcomes. The mechanisms why is a mild in some patients progresses severe often with respiratory are not known. Biomarkers that predict the course of urgently needed. aim this study was evaluate large spectrum established laboratory measurements.Patients prospective PULMPOHOM CORSAAR studies were recruited comprised 35 COVID-19, 23 conventional pneumonia, 28 control undergoing...
Aspergillus fumigatus is a fungal pathogen of major clinical importance. However, little known about the role human bronchial epithelial cells (HBECs) during A. conidia induced inflammation. Here, we show that differentiated respiratory recognise inactivated resting but not swollen or hyphae, resulting in induction interferon (IFN)-β signalling pathway and expression IFN-β-inducible genes, such as IFN-γ-inducible protein (IP)-10. This was internalisation dependent. We identified...
The receptor for advanced glycation endproducts (RAGE) is highly expressed in the lung but its physiological functions this organ still not completely understood. To determine contribution of RAGE to lung, we analyzed pulmonary mechanics and structure wildtype deficient (RAGE-/-) mice. deficiency spontaneously resulted a loss shown by an increased mean chord length, respiratory system compliance, decreased elastance concentrations serum protein albumin bronchoalveolar lavage fluids....
Chronic diseases of the respiratory tract, such as cystic fibrosis, are associated with mucosal and systemic hypoxia. Innate immune functions airway epithelial cells required to prevent control infections lung parenchyma. The transcription factor hypoxia-inducible 1α (HIF-1α) regulates cellular adaptation low oxygen conditions. Here, we show that hypoxia HIF-1α regulate innate mechanisms cultured human bronchial (HBECs). Exposure primary HBECs or prolyl hydroxylase inhibitor...
Cigarette smoke (CS) is the predominant pathogenetic factor in development of chronic bronchitis and obstructive pulmonary disease. The knowledge about cellular molecular mechanisms underlying smoke-induced inflammation epithelial cells limited.The aim this study was to develop an vitro model monitor effects volatile CS on differentiated airway cells.The cell line MM-39 primary human bronchial were cultivated as air-liquid interface cultures exposed directly CS. We used two types exposure...
Streptococcus pneumoniae and Haemophilus influenzae are human pathogens that often asymptomatically colonize the mucosal surface of upper respiratory tract, but also occasionally cause invasive disease. The ability these species to traverse epithelium airway mucosa was modeled in vitro using polarized epithelial cells culture. Migration across barrier preceded by loss transepithelial resistance. Membrane products S. included lipoteichoic acid induced disruption a Toll-like receptor...