A5016045437- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Long-Term Effects of COVID-19
- Immunotherapy and Immune Responses
- Animal Virus Infections Studies
- Mosquito-borne diseases and control
- SARS-CoV-2 detection and testing
- Malaria Research and Control
- vaccines and immunoinformatics approaches
- Viral gastroenteritis research and epidemiology
- Vaccine Coverage and Hesitancy
- Immune Cell Function and Interaction
- RNA Interference and Gene Delivery
- COVID-19 and Mental Health
- Research on Leishmaniasis Studies
- COVID-19 epidemiological studies
- Computational Drug Discovery Methods
- Advanced Drug Delivery Systems
- Virology and Viral Diseases
- Chemical Synthesis and Analysis
- Click Chemistry and Applications
- CAR-T cell therapy research
- Complement system in diseases
- Bacterial Infections and Vaccines
- Food Allergy and Anaphylaxis Research
Agency for Science, Technology and Research
2011-2025
Beijing Normal University
2025
A*STAR Graduate Academy
2021-2022
Singapore Immunology Network
2011-2022
Singapore Polytechnic
2020
Technology Centre Prague
2020
National University Health System
2016-2017
National University of Singapore
2016-2017
Background Infection with Plasmodium berghei ANKA (PbA) in susceptible mice induces a syndrome called experimental cerebral malaria (ECM) severe pathologies occurring various mouse organs. Immune mediators such as T cells or cytokines have been implicated the pathogenesis of ECM. Red blood infected PbA parasites shown to accumulate brain and other tissues during infection. This accumulation is thought be involved PbA–induced pathologies, which mechanisms are poorly understood. Methods...
Abstract Understanding the impact of age on vaccinations is essential for design and delivery vaccines against SARS-CoV-2. Here, we present findings from a comprehensive analysis multiple compartments memory immune response in 312 individuals vaccinated with BNT162b2 SARS-CoV-2 mRNA vaccine. Two vaccine doses induce high antibody T cell responses most individuals. However, recognition Spike protein Delta Omicron variants less efficient than that ancestral Wuhan strain. Age-stratified...
The immune responses and mechanisms limiting symptom progression in asymptomatic cases of SARS-CoV-2 infection remain unclear. We comprehensively characterized transcriptomic profiles, cytokine responses, neutralization capacity antibodies, cellular phenotypes patients with acute to identify potential protective mechanisms. Compared symptomatic patients, had higher counts mature neutrophils lower proportion CD169+ expressing monocytes the peripheral blood. Systemic levels pro-inflammatory...
Chitosan, a natural polysaccharide derived from chitin, possesses biocompatibility, biodegradability, and mucoadhesive characteristics, making it an attractive material for the delivery of mRNA payloads to nasal mucosa promoting their uptake by target cells such as epithelial immune (e.g., dendritic macrophages). In this project, we aimed at developing novel lipid-based nanoformulations counteract pandemic caused SARS-CoV-2 virus. The formulations achieved encapsulation efficiency ~80.2%...
Peptide-based molecules hold great potential as targeted inhibitors of intracellular protein–protein interactions (PPIs).
SARS-CoV-2 was first detected in late December 2019, however, the few months that followed, resultant COVID-19 disease has developed into a devastating pandemic around world [[1]https://covid19.who.int/.Google Scholar]. This led to race produce safe and efficacious vaccine record time. In less than year, BNT162b2, an mRNA-based by BioNTech-Pfizer [[2]Polack FP Thomas SJ Kitchin N et al.Safety efficacy of BNT162b2 mRNA Covid-19 vaccine.N Engl J Med. 2020; 383: 2603-2615Crossref PubMed Scopus...
Background/Objectives: mRNA vaccines have demonstrated strong immunogenicity and efficacy against SARS-CoV-2. However, the extent of antibody cross-reactivity human seasonal coronaviruses, such as NL63, remains unclear. Furthermore, it is unknown whether pre-existing responses NL63 might influence outcome SARS-CoV-2 vaccination. Methods: We used a flow cytometry-based serological assay an in vitro neutralization to analyze sera from mRNA-vaccinated mice plasma samples vaccinated cohort....
Abstract Objectives The emergence of a SARS‐CoV‐2 variant with point mutation in the spike (S) protein, D614G, has taken precedence over original Wuhan isolate by May 2020. With an increased infection and transmission rate, it is imperative to determine whether antibodies induced against D614 may cross‐neutralise G614 variant. Methods Antibody profiling S protein flow cytometry assessment neutralising antibody titres using pseudotyped lentiviruses expressing either or tagged luciferase...
Abstract Intranasal delivery of mRNA vaccines offers promising opportunities to combat airborne viruses like SARS‐CoV‐2 by provoking mucosal immunity, which not only defends against respiratory infection but also prevents contagious transmission. However, the development nasal has been hampered lack effective means overcome mucus barrier. Herein, ionizable lipid‐incorporated liquid lipid nanoparticles (iLLNs) capable delivering cargo across airway mucosa are designed. Adjusting ratios and...
Abstract Long‐term complications from coronavirus disease 2019 (COVID‐19) are concerning, as survivors can develop subclinical multiorgan dysfunction. It is unknown if such due to prolonged inflammation, and severe acute respiratory syndrome 2 (SARS‐CoV‐2) vaccination may reduce sequela. We conducted a prospective longitudinal study on hospitalized patients over 24 months. Clinical symptoms were collected by self‐reporting during follow‐up, along with blood samples for quantification of...
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern (VOCs) that have become dominant as the pandemic progresses bear ORF8 mutation together with multiple spike mutations. A 382-nucleotide deletion (Δ382) in ORF7b and regions has been associated milder disease phenotype less systemic inflammation COVID-19 patients. However, its impact on host immunity against SARS-CoV-2 remains undefined. Here, RNA-sequencing was performed to elucidate whole blood...
Severe SARS-CoV-2 infection can trigger uncontrolled innate and adaptive immune responses, which are commonly associated with lymphopenia increased neutrophil counts. However, whether the abnormalities observed in mild to severely infected patients persist into convalescence remains unclear. Herein, comparisons were drawn between responses of COVID-19 convalescent adults. Strikingly, survivors severe had decreased proportions NKT Vδ2 T cells, low-density neutrophils, IgA+/CD86+/CD123+...
Abstract Background On 26 November 2021, the World Health Organization designated B.1.1.529 lineage of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) as fifth variant concern, Omicron. Infections have quickly spread worldwide, but understanding viral dynamics and cytokine cellular immunological response during infection remain limited. Methods Detailed patient-level data from 174 age-matched patients with sequence confirmed Omicron or Delta admitted to National Centre for...
The cytotoxicity of epitope-specific CD8+ T cells is usually measured indirectly through IFNγ production. Existing assays that directly measure this activity are limited mainly to measurements up two specificities in a single reaction. Here, we develop multiplex assay allows direct, simultaneous measurement 23 different This can greatly reduce the amount starting clinical materials for systematic screening cell epiptopes. In addition, enhanced capacity enables incorporation irrelevant...
ABSTRACT The emergence of a SARS-CoV-2 variant with point mutation in the spike (S) protein, D614G, has taken precedence over original Wuhan isolate by May 2020. With an increased infection and transmission rate, it is imperative to determine whether antibodies induced against D614 may cross-neutralize G614 variant. In this report, profiling anti-SARS-CoV-2 humoral immunity reveals similar neutralization profiles both S protein variants, albeit waning neutralizing antibody capacity at later...
Ex vivo assay systems provide a powerful approach to studying human malaria parasite biology and testing antimalarials. For rodent parasites, short-term in vitro culture ex antimalarial susceptibility assays are relatively cumbersome, relying on passage for synchronization, since ring-stage parasites an essential starting material. Here, we describe new based the enrichment of Plasmodium berghei, P. yoelii, vinckei using single-step Percoll gradient. Importantly, demonstrate that enriched...
Artemisinin-based antimalarials, such as artesunate (ART), alone or in combination, are the mainstay of therapy against malaria caused by Plasmodium falciparum. However, emergence and spread artemisinin resistance threatens future success its global eradication. Although much reported can be attributed to mutations intrinsic parasite, a significant proportion treatment failures thought due other factors host's immune system. Exactly how system participates clearance elimination parasites...
Abstract Objective Despite the high vaccine efficacy of mRNA COVID‐19 vaccines, there are individuals who developed excessive reactogenic and/or allergic responses after first dose and were considered ineligible for further doses. CoronaVac, an inactivated SARS‐CoV‐2 vaccine, is recommended in Singapore as alternative. Methods Individuals, vaccines (BNT162b2 or mRNA‐1273) because reactive to prime vaccination, recruited offered two doses CoronaVac booster vaccination 38–224 days post their...
Abstract Waning antibody levels against severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) and the emergence of variants concern highlight need for booster vaccinations. This is particularly important elderly population, who are at a higher risk developing disease 2019 (COVID‐19) disease. While studies have shown increased responses following vaccination, understanding changes in T B cell compartments induced by third vaccine dose remains limited. We analyzed humoral cellular...