A5060741317- Enzyme Production and Characterization
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Steroid Chemistry and Biochemistry
- Alzheimer's disease research and treatments
- Pharmacogenetics and Drug Metabolism
- Enzyme Structure and Function
- Microbial Metabolism and Applications
- Enzyme-mediated dye degradation
- Protein Hydrolysis and Bioactive Peptides
- Probiotics and Fermented Foods
- Bacteriophages and microbial interactions
- Diet, Metabolism, and Disease
- Proteins in Food Systems
- Microbial Metabolites in Food Biotechnology
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Hormonal Regulation and Hypertension
- Plant biochemistry and biosynthesis
- Polyamine Metabolism and Applications
- Microbial Natural Products and Biosynthesis
- Phytase and its Applications
- Crystallization and Solubility Studies
- Gut microbiota and health
Tianjin University of Science and Technology
2016-2025
Tianjin Economic-Technological Development Area
2013-2025
Chinese Academy of Tropical Agricultural Sciences
2025
Dali University
2024
China International Science and Technology Cooperation
2022-2023
Ministry of Education of the People's Republic of China
2009-2022
State Key Laboratory of Food Nutrition and Safety
2018-2022
Institute for Fermentation
2019
Tianjin Medical University
2019
State Key Laboratory of Food Science and Technology
2018
NCBI’s Conserved Domain Database (CDD) is a resource for the annotation of protein sequences with location conserved domain footprints, and functional sites inferred from these footprints. CDD includes manually curated models that make use 3D structure to refine provide insights into sequence/structure/function relationships. Manually are organized hierarchically if they describe families clearly related by common descent. As also imports family variety external sources, it partially...
NCBI's Conserved Domain Database (CDD) is a collection of multiple sequence alignments and derived database search models, which represent protein domains conserved in molecular evolution. The can be accessed at http://www.ncbi.nlm.nih.gov/Structure/cdd/cdd.shtml, also part Entrez query retrieval system, cross-linked to numerous other resources. CDD provides annotation domain footprints functional sites on sequences. Precalculated retrieved for sequences tracked CDD's models queried with...
polysaccharides present several physiological activities including antiviral, antitumor and anti-plasmodial effects. However, current processing usually results in low yields high prices, thus lacking commercialization potential. The aim of this study was to develop an efficient method for the extraction
Abstract Nanocatalytic‐based wound therapeutics present a promising strategy for generating reactive oxygen species (ROS) to antipathogen promote healing. However, the full clinical potential of these nanocatalysts is limited by their low reactivity, targeting ability, and poor biodegradability in microenvironment. Herein, bio‐organic nanozyme developed encapsulating FeZn‐based bimetallic organic framework (MOF) (MIL‐88B‐Fe/Zn) platelet membranes (PM@MIL‐88B‐Fe/Zn) antimicrobial activity...
The outermost surface of wool is covered by a scale layer, posing challenges to some steps fabric processing. This primarily composed keratin, resists degradation conventional proteases due its high disulfide bond content. Protease K, an extracellular serine endo-proteinase derived from Tritirachium album Limber (tPRK), known for ability digest native keratin. However, limited activity against keratin has restricted application in layer treatment. In this study, the substrate-binding pocket...
The fibrillogenesis of amyloid-β protein (Aβ) is considered a crucial factor in the pathogenesis Alzheimer's disease (AD). Hence, inhibiting Aβ regarded as primary therapeutic strategy for prevention and treatment AD. However, development effective inhibitors against has faced significant challenges. Previous studies have shown that pristine single-walled carbon nanotubes (SWNTs) can inhibit some amyloid proteins. poor dispersibility SWNTs an aqueous environment greatly hinders their...
Signal peptides are short directing newly synthesized proteins toward the secretory pathway. These N-terminal signal sequences ubiquitous to all prokaryotes and eukaryotes. play a significant role in recombinant protein production. Previous studies have demonstrated that secretion amount of given target varies significantly depending on peptide is fused protein. selection modification two main methods for optimization secretion. However, highly efficient with specific bacterial expression...
The versatile synthetic intermediate (2S,4R)-4-hydroxylysine can be produced using l-lysine hydroxylase. However, the wild-type enzyme cannot effectively catalyze C4 hydroxylation of to form product. To overcome this bottleneck, we modified hydroxylase from Niastella koreensis (NkLH4), semirational combinatorial active-site saturation test (CAST). We obtained a highly active mutant MT3 (Q161N/T162A/F178Y/E260D) with 24.97-fold increase kcat/Km, compared (791.33 mM–1 s–1 vs 31.69 s–1)....
Biosynthesis of D-allulose has been achieved using ketose 3-epimerases (KEases), but its application is limited by poor catalytic performance. In this study, we redesigned a genetically encoded biosensor based on D-allulose-responsive transcriptional regulator for real-time monitoring D-allulose. An ultrahigh-throughput droplet-based microfluidic screening platform was further constructed coupling with D-allulose-detecting the directed evolution KEases. Structural analysis Sinorhizobium...
Hesperetin was identified to be capable of inhibiting Aβ fibrillogenesis, disassembling mature fibrils, reducing the cytotoxicity and protecting C. elegans , has good application prospects as a neuroprotective therapeutic agent for AD.
In this work, lactoferrin (LF)–chitosan (CS) composite hydrogels with good loading capacity of thermosensitive bioactive substances were successfully obtained by microbial transglutaminase (MTG)-induced cross-linking. We evaluated the rheological, textural, and microstructural characteristics under different conditions. The results demonstrated that concentrations LF CS as well amount MTG could regulate textural properties, rheological water holding capability. FTIR fluorescence spectroscopy...