A5015794568- CRISPR and Genetic Engineering
- Microbial Natural Products and Biosynthesis
- Advanced biosensing and bioanalysis techniques
- RNA regulation and disease
- RNA and protein synthesis mechanisms
- biodegradable polymer synthesis and properties
- Biosensors and Analytical Detection
- Plant biochemistry and biosynthesis
- Biochemical and Structural Characterization
- Computational Drug Discovery Methods
- Cancer-related molecular mechanisms research
- Genetic and phenotypic traits in livestock
- SARS-CoV-2 detection and testing
- Alkaloids: synthesis and pharmacology
- Microplastics and Plastic Pollution
- Fungal Biology and Applications
- RNA Interference and Gene Delivery
- Advanced Photocatalysis Techniques
- Quinazolinone synthesis and applications
- Carbon dioxide utilization in catalysis
- Plant-Microbe Interactions and Immunity
- Peptidase Inhibition and Analysis
- Biochemical and Molecular Research
- Microbial Metabolic Engineering and Bioproduction
- Plant Virus Research Studies
University of Pennsylvania
2024-2025
Air Force Medical University
2022-2025
Xijing Hospital
2022-2025
Hainan University
2024
Jilin Agricultural University
2024
Rice University
2020-2024
Jiangsu University
2024
Chinese Academy of Sciences
2024
Huzhou Vocational and Technical College
2024
Southwestern Institute of Physics
2024
Significance The therapeutic potential of protein-based genome editing is dependent on the delivery proteins to appropriate intracellular targets. Here we report that combining bioreducible lipid nanoparticles and negatively supercharged Cre recombinase or anionic Cas9:single-guide (sg)RNA complexes drives self-assembly for potent protein editing. design lipids facilitates degradation inside cells in response reductive environment, enhancing endosome escape protein. In addition, modulation...
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems have recently received notable attention for their applications in nucleic acid detection. Despite many attempts, the majority of current CRISPR-based biosensors infectious respiratory disease diagnostic still require target preamplifications. This study reports a new biosensor amplification-free detection via harnessing trans-cleavage mechanism Cas13a and ultrasensitive graphene...
Tremorgenic mycotoxins are a group of indole alkaloids which include the quinazoline-containing tryptoquivaline (2) that capable eliciting intermittent or sustained tremors in vertebrate animals. The biosynthesis this bioactive compounds, characterized by an acetylated quinazoline ring connected to 6-5-5 imidazoindolone system via 5-membered spirolactone, has remained uncharacterized. Here, we report identification gene cluster (tqa) from P. aethiopicum is involved tryptoquialanine (1),...
Engineered base editors convert the second nucleotide C to T in context of 5′-CC-3′ with high precision and targeting fidelity.
Abstract Current base- and prime-editing technologies lack efficient strategies to edit multiple genomic loci simultaneously, limiting their applications in complex genomics polygenic diseases. Here, we describe drive-and-process (DAP) CRISPR array architectures for multiplex base-editing (MBE) (MPE) human cells. We leverage tRNA as the RNA polymerase III promoter drive expression of tandemly assembled tRNA-guide (gRNA) arrays, which individual gRNAs are released by cellular endogenous...
DNAzyme-based signal amplification circuits promote the advances in low-abundant miRNA imaging living cells. However, due to insufficient cofactor cells and unsustainable target utilization, self-powered self-feedback DNAzyme have rarely been achieved. Here, a MnO2 nanosheet-mediated entropy-driven catalyst (EDC)–DNAzyme nanoprobe (MnPFEDz) was demonstrated for sensitive of intracellular microRNA (miRNA). In this strategy, MnPFEDz formed by adsorbing EDC modules substrate probes on...
Abstract Extracellular vesicles (EVs) have inherent advantages over cell‐based therapies in regenerative medicine because of their cargos abundant bioactive cues. Several strategies are proposed to tune EVs production vitro. However, it remains a challenge for manipulation vivo, which poses significant difficulties EVs‐based that aim promote tissue regeneration, particularly long‐term treatment diseases like peripheral neuropathy. Herein, superparamagnetic nanocomposite scaffold capable...
Quantitative polymerase chain reaction as a powerful tool for DNA detection has been pivotal to vast range of applications, including disease screening, food safety assessment, environmental monitoring, and many others. However, the essential target amplification step in combination with fluorescence readout poses significant challenge rapid streamlined analysis. The discovery engineering clustered regularly interspaced short palindromic repeats (CRISPR) CRISPR-associated (Cas) technology...
Mutations in Atp2b2, an outer hair cell gene, cause dominant hearing loss humans. Using a mouse model Atp2b2
Tetracyclines are a group of natural products sharing linearly fused four-ring scaffold, which is essential for their broad-spectrum antibiotic activities. Formation the key precursor anhydrotetracycline 3 during oxytetracycline 1 biosynthesis has been previously characterized. However, enzymatic steps that transform into 1, including additional hydroxylation at C5 and final C5a–C11a reduction, have remained elusive. Here we report two redox enzymes, OxyS OxyR, sufficient to convert 1....
Mycotoxin contamination causes disease and death in both humans animals. Monascus Red, produced by purpureus, is used as a food colorant. However, its application limited of the nephrotoxin citrinin, which also fungus. Suppressing citrinin production genetic engineering difficult polykaryotic fungus such M. purpureus. Hence, we developed CRISPR/Cas system to delete large genomic fragments fungi. Protoplast preparation regeneration were optimized, dual-plasmid was designed enable deletion...
Abstract The specificity of CRISPR/Cas9 genome editing is largely determined by the sequences guide RNA (gRNA) and targeted DNA, yet sequence-dependent rules underlying off-target effects are not fully understood. To systematically explore sequence determinants governing specificity, here we describe a dual-target system to measure relative cleavage rate between off- on-target (off-on ratios) 1902 gRNAs on 13,314 synthetic target sequences, reveal set involving 2 factors in off-targeting: 1)...