A5052946108- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Nanoplatforms for cancer theranostics
- DNA and Nucleic Acid Chemistry
- Biosensors and Analytical Detection
- Angiogenesis and VEGF in Cancer
- Advanced Nanomaterials in Catalysis
- Graphene and Nanomaterials Applications
- Electrochemical sensors and biosensors
- Concrete and Cement Materials Research
- Advanced Photocatalysis Techniques
- Supramolecular Self-Assembly in Materials
- MicroRNA in disease regulation
- Innovative concrete reinforcement materials
- Luminescence Properties of Advanced Materials
- Nanoparticle-Based Drug Delivery
- Ovarian cancer diagnosis and treatment
- Nanocluster Synthesis and Applications
- Carbon and Quantum Dots Applications
- Luminescence and Fluorescent Materials
- Bacteriophages and microbial interactions
- Bone Tissue Engineering Materials
- Polymer Surface Interaction Studies
- Immunotherapy and Immune Responses
- Electrochemical Analysis and Applications
Fuzhou University
2016-2025
Nanjing Tech University
2011-2025
Jiangsu University
2010-2025
Fujian Medical University
2024-2025
Indiana University School of Medicine
2025
The Synergetic Innovation Center for Advanced Materials
2022-2024
First Affiliated Hospital of Fujian Medical University
2024
The University of Texas MD Anderson Cancer Center
2008-2023
China University of Geosciences
2021-2023
Peking University
2023
Sensitive platform: The use of graphene oxide (GO) as a platform for the sensitive and selective detection DNA proteins is presented. interaction GO dye-labeled single-stranded leads to quenching dye fluorescence. Conversely, presence target or protein binding target, releasing from GO, thereby restoring fluorescence (see picture).
Abstract Auf Graphenoxid (GO) beruht ein empfindliches und selektives Verfahren zum DNA‐ Proteinnachweis. Durch die Wechselwirkung von GO mit einer farbstoffmarkierten Einzelstrang‐DNA wird Fluoreszenz des Farbstoffs gelöscht. Sind Ziel‐DNAs oder Zielproteine vorhanden, binden sie an farbstoffmarkierte DNA spalten ab, sodass erneut fluoresziert (siehe Bild). magnified image
Nucleic acid-functionalized polyacrylamide chains that are cooperatively cross-linked by i-motif and nucleic acid duplex units yield, at pH 5.0, DNA hydrogels exhibiting shape-memory properties. Separation of the 8.0 dissolves hydrogel into a quasi-liquid phase. The residual provide, however, memory code in allowing regeneration shape 5.0.
We have proved that functionalized nanoscale graphene oxide can protect oligonucleotides from enzymatic cleavage and efficiently deliver into cells.
Abstract RNA interference (RNAi) is a powerful approach for silencing genes associated with variety of pathologic conditions; however, in vivo RNAi delivery has remained major challenge due to lack safe, efficient, and sustained systemic delivery. Here, we report on novel overcome these limitations using multistage vector composed mesoporous silicon particles (stage 1 microparticles, S1MP) loaded neutral nanoliposomes (dioleoyl phosphatidylcholine, DOPC) containing small interfering (siRNA)...
This communication describes the design of a novel and general bioresponsive controlled-release mesoporous silica (MS) nanoparticles system based on aptamer−target interactions. In this system, pores MS were capped with Au modified aptamer (ATP in case). By competitive displacement reaction, uncapped presence ATP molecule, cargo was released. Our results demonstrated that interaction may be promising route for custom-made nanodevices specifically governed by target biomolecules. Since...
Abstract Purpose: This study aimed to develop an Arg-Gly-Asp (RGD) peptide-labeled chitosan nanoparticle (RGD-CH-NP) as a novel tumor targeted delivery system for short interfering RNA (siRNA). Experimental Design: RGD peptide conjugated with by thiolation reaction was confirmed proton-NMR (H-NMR). Binding of RGD-CH-NP ανβ3 integrin examined flow cytometry and fluorescence microscopy. Antitumor efficacy in orthotopic mouse models ovarian carcinoma. Results: We show that loaded siRNA...
In this work, we report a facile approach for imprinting protein based on self-polymerization of dopamine in the presence template Fe3O4 nanoparticles (NPs) surface. Dopamine, commonly known as neurotransmitter, is also small-molecule mimic adhesive proteins mussels. Self-polymerization can produce thin polydopamine (PDA) layer NPs During dopamine, some molecules were embedded PDA layer. After removal these molecules, imprinted sites are created. The prepared Fe3O4@PDA shows high binding...
DNA/nanoparticle hybrid systems combine the unique electronic and optical properties of nanomaterials with recognition catalytic nucleic acids. These materials hold great promise for development new sensing platforms, programmed organization nanoparticles, switchable control plasmonic phenomena in nanostructures, controlled delivery drugs. In this Perspective, we summarize recent advances application (NP) hybrids these different disciplines. Nucleic acid–semiconductor quantum dot are...
A method to assemble light-responsive or pH-responsive microcapsules loaded with different loads (tetramethylrhodamine-modified dextran, TMR-D; microperoxidase-11, MP-11; CdSe/ZnS quantum dots; doxorubicin-modified DOX-D) is described. The based on the layer-by-layer deposition of sequence-specific nucleic acids poly(allylamine hydrochloride)-functionalized CaCO3 core microparticles, loads, that after dissolution particles EDTA yields stimuli-responsive include respective loads. are composed...
Abstract The use of hydrogel‐based bone adhesives has the potential to revolutionize clinical treatment repairs. However, severe deficiencies remain in current products, including cytotoxicity concerns, inappropriate mechanical strength, and poor fixation performance wet biological environments. Inspired by unique roles glue molecules robust strength fracture resistance bone, a design strategy is proposed using novel mineral–organic for strong water‐resistant guided tissue regeneration....
Turn up the volume: In a simple and highly sensitive aptamer-based assay that relies on ability of nanomaterials to protect DNA from nuclease cleavage, aptamer is released nanomaterial when challenged with target. The then cleaves free releases target, which can initiate new cycle. Recycling target in this way leads significant signal amplification (see diagram).
RNA interference holds tremendous potential as a therapeutic approach, especially in the treatment of malignant tumors. However, efficient and biocompatible delivery methods are needed for systemic small interfering (siRNA). To maintain high level growth, tumor cells scavenge high-density lipoprotein (HDL) particles by overexpressing its receptor: scavenger receptor type B1 (SR-B1). In this study, we exploited cellular characteristic to achieve siRNA established novel formulation...
Surgical stress has been suggested to facilitate the growth of preexisting micrometastases as well small residual tumor postoperatively. The purpose this study was examine effects surgical on ovarian cancer and determine underlying mechanisms responsible for increased growth.To mimic surgery, we did a laparotomy or mastectomy under isoflurane inhalation athymic nude mice 4 days after i.p. cell injection. Propranolol infusion via Alzet pumps used block influence sympathetic nervous system...
Abstract Focal adhesion kinase (FAK) overexpression is frequently found in ovarian and other cancers predictive of poor clinical outcome. In the current study, we characterized biological therapeutic effects a novel FAK inhibitor, TAE226. Taxane-sensitive (SKOV3ip1 HeyA8) taxane-resistant (HeyA8-MDR) cell lines were used for vitro vivo therapy experiments using TAE226 alone combination with docetaxel. Assessment cytotoxicity, proliferation [proliferating nuclear antigen (PCNA)], angiogenesis...
Streptomyces aureofaciens CMUAc130 was isolated from the root tissue of Zingiber officinale Rosc. (Zingiberaceae). It an antagonist Colletotrichum musae and Fusarium oxysporum , causative agents anthracnose banana wilt wheat, respectively. Evidence for in vitro antibiosis S. demonstrated by zone fungal-growth inhibition. Microscopic observations showed thickness bulbous structures at edges inhibited fungal hyphae. The culture filtrate crude extract this strain were all inhibitory to tested...
In this paper, we present a general protocol for the making of surface-imprinted core−shell nanoparticles via surface reversible addition−fragmentation chain-transfer (RAFT) polymerization using RAFT agent functionalized model silica as agent. protocol, trichloro(4-chloromethylphenyl)silane was immobilized on SiO2 nanoparticles, forming chloromethylphenyl (silica-Cl). subsequently produced by substitute reaction silica-Cl with PhC(S)SMgBr. The grafting copolymerization 4-vinylpyridine and...
Here, we report a novel, highly sensitive, selective and economical molecular beacon using graphene oxide as the "nanoquencher". This novel system contains hairpin-structured fluorophore-labeled oligonucleotide sheet. The strong interaction between keep them in close proximity, facilitating fluorescence quenching of fluorophore by oxide. In presence complementary target DNA, binding DNA will disturb oxide, release from resulting restoration fluorescence. present study, show that this...
Therapeutic strategies based on antiangiogenic approaches are beginning to show great promise in clinical studies. However, full realization of these requires identification key differences gene expression between endothelial cells from tumors versus their normal counterparts. Here, we examined purified 10 invasive epithelial ovarian cancers and 5 ovaries using Affymetrix U133 Plus 2.0 microarrays. More than 400 differentially expressed genes were identified tumor-associated cells. We...