A5054033791- Ovarian cancer diagnosis and treatment
- Endometrial and Cervical Cancer Treatments
- Angiogenesis and VEGF in Cancer
- Cardiac, Anesthesia and Surgical Outcomes
- Kruppel-like factors research
- Axon Guidance and Neuronal Signaling
- Inflammatory Biomarkers in Disease Prognosis
- Cancer, Stress, Anesthesia, and Immune Response
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Uterine Myomas and Treatments
- Cancer, Lipids, and Metabolism
- Enhanced Recovery After Surgery
- Cancer, Hypoxia, and Metabolism
- Cell Adhesion Molecules Research
- RNA modifications and cancer
- Blood properties and coagulation
- Cancer Mechanisms and Therapy
- Cancer Research and Treatments
- Intraperitoneal and Appendiceal Malignancies
- Extracellular vesicles in disease
- Minimally Invasive Surgical Techniques
- Anesthesia and Pain Management
- Epigenetics and DNA Methylation
Texas Oncology
2023-2024
The University of Texas MD Anderson Cancer Center
2011-2023
Gynecologic Oncology Group
2007-2023
Saint Thomas Health
2018-2023
Tennessee Oncology
2019-2023
Heidelberg University
2023
University Hospital Heidelberg
2023
University Medical Centre Mannheim
2023
St. Thomas Medical Group
2016-2021
LMU Klinikum
2020
The mechanisms of paraneoplastic thrombocytosis in ovarian cancer and the role that platelets play abetting growth are unclear.We analyzed clinical data on 619 patients with epithelial to test associations between platelet counts disease outcome. Human samples mouse models were used explore underlying thrombocytosis. effects tumor angiogenesis ascertained.Thrombocytosis was significantly associated advanced shortened survival. Plasma levels thrombopoietin interleukin-6 elevated who had as...
We studied Dicer and Drosha, components of the RNA-interference machinery, in ovarian cancer.We measured messenger RNA (mRNA) levels Drosha specimens invasive epithelial cancer from 111 patients, using a quantitative reverse-transcriptase-polymerase-chain-reaction assay, compared results with clinical outcomes. Validation was performed use published microarray data cohorts patients ovarian, breast, lung cancer. Mutational analyses genomic DNA genes were subgroup ovarian-cancer specimens....
The “Guidelines for Pre- and Intra-operative Care in Gynecologic/Oncology Surgery: Enhanced Recovery After Surgery (ERAS®) Society Recommendations — Part I,” [1] examined the evidence surrounding care elements including preoperative medical optimization, bowel preparation, carbohydrate loading, thromboembolism prophylaxis, skin standard anesthetic protocol intraoperative fluid management. goal of this article is to critically review existing make recommendations postoperative care.
•We provide evidence supporting pre- and intra-operative management of patients undergoing gynecologic/oncology surgery.•This guideline will help integrate knowledge into practice, align perioperative care, encourage future investigations.
Aldehyde dehydrogenase-1A1 (ALDH1A1) expression characterizes a subpopulation of cells with tumor-initiating or cancer stem cell properties in several malignancies. Our goal was to characterize the phenotype ALDH1A1-positive ovarian and examine biological effects ALDH1A1 gene silencing. In our analysis multiple lines, we found that activity significantly higher taxane- platinum-resistant lines. patient samples, 72.9% cancers had which percentage correlated negatively progression-free...
Abstract RNA interference (RNAi) is a powerful approach for silencing genes associated with variety of pathologic conditions; however, in vivo RNAi delivery has remained major challenge due to lack safe, efficient, and sustained systemic delivery. Here, we report on novel overcome these limitations using multistage vector composed mesoporous silicon particles (stage 1 microparticles, S1MP) loaded neutral nanoliposomes (dioleoyl phosphatidylcholine, DOPC) containing small interfering (siRNA)...
Abstract Purpose: This study aimed to develop an Arg-Gly-Asp (RGD) peptide-labeled chitosan nanoparticle (RGD-CH-NP) as a novel tumor targeted delivery system for short interfering RNA (siRNA). Experimental Design: RGD peptide conjugated with by thiolation reaction was confirmed proton-NMR (H-NMR). Binding of RGD-CH-NP ανβ3 integrin examined flow cytometry and fluorescence microscopy. Antitumor efficacy in orthotopic mouse models ovarian carcinoma. Results: We show that loaded siRNA...
Chronic stress is associated with hormonal changes that are known to affect multiple systems, including the immune and endocrine but effects of on cancer growth progression not fully understood. Here, we demonstrate human ovarian cells exposed either norepinephrine or epinephrine exhibit lower levels anoikis, process by which enter apoptosis when separated from ECM neighboring cells. In an orthotopic mouse model cancer, restraint increases in protected tumor anoikis promoted their activating...
BACKGROUND Preclinical evidence has suggested that sustained adrenergic activation can promote ovarian cancer growth and metastasis. The authors examined the impact of beta‐adrenergic blockade on clinical outcome women with epithelial ovarian, primary peritoneal, or fallopian tube cancers (collectively, [EOC]). METHODS A multicenter review 1425 histopathologically confirmed EOC was performed. Comparisons were made between patients documented beta‐blocker use during chemotherapy those without...
Metastasis is a complex, multistep process that begins with the epithelial-mesenchymal transition (EMT). Circulating tumor cells (CTC) are believed to have undergone EMT and thus lack or express low levels of epithelial markers commonly used for enrichment and/or detection such cells. However, most current CTC methods target only EpCAM cytokeratin (CK) enrich CTCs, resulting in failure recognize other, perhaps more important, phenotypes expression these markers. Here, we describe population...
Surgical stress has been suggested to facilitate the growth of preexisting micrometastases as well small residual tumor postoperatively. The purpose this study was examine effects surgical on ovarian cancer and determine underlying mechanisms responsible for increased growth.To mimic surgery, we did a laparotomy or mastectomy under isoflurane inhalation athymic nude mice 4 days after i.p. cell injection. Propranolol infusion via Alzet pumps used block influence sympathetic nervous system...
Abstract Photothermal ablation (PTA) is an emerging technique that uses near-infrared (NIR) laser light–generated heat to destroy tumor cells. However, complete eradication by PTA therapy alone difficult because heterogeneous distribution can lead sublethal thermal dose in some areas of the tumor. Successful requires selective delivery photothermal conducting nanoparticles mediate effective cells, and ability combine with other modalities. Here, we synthesized multifunctional doxorubicin...
Abstract Focal adhesion kinase (FAK) overexpression is frequently found in ovarian and other cancers predictive of poor clinical outcome. In the current study, we characterized biological therapeutic effects a novel FAK inhibitor, TAE226. Taxane-sensitive (SKOV3ip1 HeyA8) taxane-resistant (HeyA8-MDR) cell lines were used for vitro vivo therapy experiments using TAE226 alone combination with docetaxel. Assessment cytotoxicity, proliferation [proliferating nuclear antigen (PCNA)], angiogenesis...
Resistance to platinum chemotherapy remains a significant problem in ovarian carcinoma. Here, we examined the biological mechanisms and therapeutic potential of targeting critical resistance gene, ATP7B, using both vitro vivo models.Expression ATP7A ATP7B was cancer cell lines by real-time reverse transcription-PCR Western blot analysis. gene silencing achieved with targeted small interfering RNA (siRNA) its effects on viability DNA adduct formation were examined. For therapy experiments,...
Abstract Vascular endothelial growth factor receptor (VEGFR) has recently been discovered on ovarian cancer cells, but its functional significance is unknown and the focus of this study. By protein analysis, A2780‐par HeyA8 cell lines expressed VEGFR‐1 A2774, SKOV3ip1 VEGFR‐2. in situ hybridization (ISH), 85% human specimens showed moderate to high VEGFR‐2 expression, whereas only 15% expression. immunofluorescence, little or no was detected normal surface epithelial expression 75% invasive...
To investigate the effect of an enhanced recovery after surgery (ERAS) program on perioperative outcomes with emphasis opioid consumption and patient-reported in immediate extended postoperative periods.We initiated our ERAS as part a quality improvement initiative November 2014. We compared clinical among cohort 607 women undergoing open gynecologic before or implementation ERAS. For 293 patients, were using MD Anderson Symptom Inventory-Ovarian Cancer.Median age was 58 years (range 18-85...
Abstract Purpose: Jagged1, a Notch ligand, is expressed on both tumor epithelial and endothelial cells therefore may be amenable to dual targeting of the stroma malignant cell compartments microenvironment. Experimental Design: We describe in vitro effects Jagged1 ovarian cancer vivo independent stromal using species-specific human or murine siRNA constructs incorporated into chitosan nanoparticles delivered intravenously an orthotopic mouse model. Results: expression was prominent SKOV3ip1...
Recent studies in patients with ovarian cancer suggest that tumor growth may be accelerated following cessation of antiangiogenesis therapy; however, the underlying mechanisms are not well understood. In this study, we aimed to compare effects therapy withdrawal those continuous treatment various antiangiogenic agents. Cessation pazopanib, bevacizumab, and human murine anti-VEGF antibody B20 was associated substantial mouse models cancer. Increased accompanied by hypoxia, increased...