A5024804165- Cancer Immunotherapy and Biomarkers
- Cancer Genomics and Diagnostics
- Ferroptosis and cancer prognosis
- Gene expression and cancer classification
- Estrogen and related hormone effects
- Immune cells in cancer
- Cancer Cells and Metastasis
- Bioinformatics and Genomic Networks
- Lung Cancer Treatments and Mutations
- RNA modifications and cancer
- Immunotherapy and Immune Responses
- Cancer, Hypoxia, and Metabolism
- Cancer, Stress, Anesthesia, and Immune Response
- Lung Cancer Research Studies
- Breast Cancer Treatment Studies
- Cancer-related Molecular Pathways
- Epigenetics and DNA Methylation
- Iron Metabolism and Disorders
- TGF-β signaling in diseases
- Glioma Diagnosis and Treatment
- Single-cell and spatial transcriptomics
- Advanced Breast Cancer Therapies
- Genomics and Chromatin Dynamics
- Intraperitoneal and Appendiceal Malignancies
- Acute Myeloid Leukemia Research
Wake Forest University
2016-2025
Atrium Health Wake Forest Baptist
2016-2025
Wake Forest Baptist Comprehensive Cancer Center
2015-2024
Genome Institute of Singapore
2002-2023
Forest Institute
2021-2023
Centre for Cancer Biology
2023
The University of Texas MD Anderson Cancer Center
2010-2017
Chinese Academy of Medical Sciences & Peking Union Medical College
2017
Peking Union Medical College Hospital
2017
Cancer Institute (WIA)
2017
Perturbations of the p53 pathway are associated with more aggressive and therapeutically refractory tumors. However, molecular assessment status, by using sequence analysis immunohistochemistry, incomplete assessors functional effects. We posited that transcriptional fingerprint is a definitive downstream indicator function. Herein, we analyzed transcript profiles 251 p53-sequenced primary breast tumors identified clinically embedded 32-gene expression signature distinguishes p53-mutant...
Adjuvant breast cancer therapy significantly improves survival, but overtreatment and undertreatment are major problems. Breast expression profiling has so far mainly been used to identify women with a poor prognosis as candidates for adjuvant without demonstrated value prediction.We obtained the gene profiles of 159 population-derived patients, hierarchical clustering signature associated impact therapies, defined distant metastasis or death within 5 years. Independent datasets 76 treated...
Abstract Histologic grading of breast cancer defines morphologic subtypes informative metastatic potential, although not without considerable interobserver disagreement and clinical heterogeneity particularly among the moderately differentiated grade 2 (G2) tumors. We posited that a gene expression signature capable discerning tumors 1 (G1) 3 (G3) histology might provide more objective measure with prognostic benefit for patients G2 disease. To this end, we studied profiles 347 primary...
Using a chromatin immunoprecipitation-paired end diTag cloning and sequencing strategy, we mapped estrogen receptor alpha (ERalpha) binding sites in MCF-7 breast cancer cells. We identified 1,234 high confidence clusters of which 94% are projected to be bona fide ERalpha regions. Only 5% the located within 5 kb upstream transcriptional start adjacent genes, regions containing proximal promoters, whereas vast majority intronic or distal locations (>5 from 5' 3' ends transcript), suggesting...
An iron efflux pump is a pivotal protein in breast cancer biology and strong independent predictor of prognosis.
Aldehyde dehydrogenase-1A1 (ALDH1A1) expression characterizes a subpopulation of cells with tumor-initiating or cancer stem cell properties in several malignancies. Our goal was to characterize the phenotype ALDH1A1-positive ovarian and examine biological effects ALDH1A1 gene silencing. In our analysis multiple lines, we found that activity significantly higher taxane- platinum-resistant lines. patient samples, 72.9% cancers had which percentage correlated negatively progression-free...
Ferroportin and hepcidin are critical proteins for the regulation of systemic iron homeostasis. is only known mechanism export intracellular non–heme-associated iron; its stability regulated by hormone hepcidin. Although ferroportin profoundly affects concentrations in tissues important absorption trafficking, breast cancer their influence on growth prognosis have not been examined. We demonstrate here that both expressed cultured human epithelial cells regulates these cells. Further,...
Mounting evidence supports a role for the immune system in breast cancer outcomes. The ability to distinguish highly immunogenic tumors susceptible anti-tumor immunity from weakly or inherently immune-resistant would guide development of therapeutic strategies cancer. Genomic, transcriptomic and clinical data Cancer Genome Atlas (TCGA) Molecular Taxonomy Breast International Consortium (METABRIC) cohorts were used examine statistical associations between tumor mutational burden (TMB)...
The lack of multi-omics cancer datasets with extensive follow-up information hinders the identification accurate biomarkers clinical outcome. In this cohort study, we performed comprehensive genomic analyses on fresh-frozen samples from 348 patients affected by primary colon cancer, encompassing RNA, whole-exome, deep T cell receptor and 16S bacterial rRNA gene sequencing tumor matched healthy tissue, complemented whole-genome for further microbiome characterization. A type 1 helper cell,...
Infection with high-risk human papillomaviruses (HPV) is a major risk factor for development of cervical cancer. Expression the HPV E6 and E7 oncoproteins increases in differentiating keratinocytes, resulting inactivation p53 retinoblastoma proteins, two important transcriptional regulators. We used cDNA microarrays to examine global alterations gene expression keratinocytes after infection retroviruses encoding type 16 (HPV-16) E7. 80 cellular genes (approximately 4% on array) was altered...
Zebrafish (Danio rerio) is a well-recognized model for the study of vertebrate developmental genetics, yet at same time little known about transcriptional events that underlie zebrafish embryogenesis. Here we have employed microarray analysis to temporal activity developmentally regulated genes during Transcriptome 12 different embryonic points covering five stages (maternal, blastula, gastrula, segmentation, and pharyngula) revealed highly dynamic profile. Hierarchical clustering,...
Molecular markers and the rich biological information they contain have great potential for cancer diagnosis, prognostication therapy prediction. So far, however, not superseded routine histopathology staging criteria, partly because few studies performed on molecular subtyping had little validation limited clinical characterization.We obtained gene expression data 412 breast cancers from population-based cohorts of patients Stockholm Uppsala, Sweden. Using intrinsic set approximately 500...
Interleukin-2 (IL-2) has direct pluripotent effects on cells with immune and inflammatory function. Which of these a critical role in mediating tumor regression remains enigmatic. In this study, we compared early changes transcriptional profiles circulating mononuclear those occurring within the microenvironment melanoma metastases following systemic IL-2 administration.The results suggest that immediate administration is activation genes predominantly associated monocyte cell function;...
Abstract Changes in iron regulation characterize the malignant state. However, pathways that effect these changes and their specific impact on prognosis remain poorly understood. We capitalized publicly available microarray datasets comprising 674 breast cancer cases to systematically investigate how expression of genes related metabolism is linked prognosis. Of 61 involved regulation, 49% were statistically significantly associated with distant metastasis-free survival. Cases divided into...
Gene expression signatures indicative of tumor proliferative capacity and tumor-immune cell interactions have emerged as principal biology-driven predictors breast cancer outcomes. How these relate to one another in biological prognostic contexts remains be clarified. To investigate the relationship between proliferation immune gene signatures, we analyzed an integrated dataset 1,954 clinically annotated profiles randomized into training test sets allow two-way discovery validation...
Abstract Liver cancers, including hepatocellular carcinomas (HCCs), cholangiocarcinomas (CCs), and fibrolamellar HCCs (FL-HCCs) are among the most common cancers worldwide associated with a poor prognosis. Investigations of genes important in liver have focused on Sal-like protein 4 (SALL4), member family zinc finger transcription factors. It is regulator embryogenesis, organogenesis, pluripotency, can elicit reprogramming somatic cells, marker stem cells. We found it expressed normal murine...
Abstract The beneficial effect of the selective estrogen receptor (ER) modulator tamoxifen in treatment and prevention breast cancer is assumed to be through its ability antagonize stimulatory actions estrogen, although can also have some estrogen-like agonist effects. Here, we report that, addition these mixed agonist/antagonist actions, selectively regulate a unique set >60 genes, which are minimally regulated by estradiol (E2) or raloxifene ERα-positive MCF-7 human cells. This gene...