A5059923178- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- RNA modifications and cancer
- Renal and related cancers
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- Cancer Cells and Metastasis
- Immune Cell Function and Interaction
- Protein Kinase Regulation and GTPase Signaling
- Mesenchymal stem cell research
- T-cell and B-cell Immunology
- Cancer Genomics and Diagnostics
- Genomics and Chromatin Dynamics
- Cancer, Hypoxia, and Metabolism
- Circular RNAs in diseases
- Tissue Engineering and Regenerative Medicine
- Erythrocyte Function and Pathophysiology
- Lung Cancer Treatments and Mutations
- Pancreatic function and diabetes
- Neonatal Respiratory Health Research
Genome Institute of Singapore
2012-2025
Sana Biotechnology (United States)
2021-2024
University of Ulsan
2024
Asan Medical Center
2024
Ulsan College
2024
Palawan State University
2023
Merck (Singapore)
2016-2021
Agency for Science, Technology and Research
2006-2020
Merck & Co., Inc., Rahway, NJ, USA (United States)
2015-2020
Harvard University
2006-2016
Embryonic stem cells (ESCs) are pluripotent that can either self-renew or differentiate into many cell types. Oct4 and Sox2 transcription factors essential to the self-renewing phenotypes of ESCs. Both upstream in hierarchy regulatory network partners regulating several ESC-specific genes. In ESCs, is transcriptionally regulated by an enhancer containing a composite sox-oct element bind combinatorial interaction. It has previously been shown Pou5f1, gene, contains distal imparting specific...
The p53 transcription factor is a key tumor suppressor and central regulator of the stress response. To ensure robust precise response to cellular signals, gene expression must be tightly regulated from transcriptional post-translational levels. Computational predictions suggest that several microRNAs are involved in post-transcriptional regulation . Here we demonstrate miR-125b, brain-enriched microRNA, bona fide negative both zebrafish humans. miR-125b-mediated down-regulation strictly...
OBJECTIVE We investigated the regulation and involvement of microRNAs (miRNAs) in fat cell development obesity. RESEARCH DESIGN AND METHODS Using miRNA microarrays, we profiled expression >370 miRNAs during adipogenesis preadipocyte 3T3-L1 cells adipocytes from leptin deficient ob/ob diet-induced obese mice. Changes key were validated by RT-PCR. further assessed contribution chronic inflammatory environment adipose tissue to dysregulated tumor necrosis factor (TNF)-α treatment...
Abstract The de novo generation of Foxp3+ regulatory T (Treg) cells in the peripheral immune compartment and differentiation Th17 both require TGF-β, IL-6 IL-21 are switch factors that drive development at expense Treg cell generation. major vitamin A metabolite all-trans retinoic acid (RA) not only enforces but also inhibits cells. Herein we show RA enhances TGF-β signaling by increasing expression phosphorylation Smad3, this results increased Foxp3 even presence or IL-21. IL-6Rα, IRF-4,...
Abstract Mesenchymal stem cells derived from human bone marrow (hBMSCs) and adipose tissue (hAMSCs) represent a useful source of progenitor for cell therapy engineering. However, it is not clear what the similarities differences between them are. Like hBMSCs, hAMSCs can differentiate into osteogenic, adipogenic, chondrogenic cells. Whether MSCs different sources fundamentally similar or types clear. Given possible therapy, comprehensive comparison would be very useful. Here, we compared...
The genetic networks controlling stem cell identity are the focus of intense interest, due to their obvious therapeutic potential as well exceptional relevance models early development. Genome-wide mapping transcriptional in mouse embryonic cells (mESCs) reveals that many endogenous noncoding RNA molecules, including long RNAs (lncRNAs), may play a role pluripotent state. We performed genome-wide screen combined full-length mESC transcriptome genomic data with chromatin immunoprecipitation...
Background The development of metastases involves the dissociation cells from primary tumor to penetrate basement membrane, invade and then exit vasculature seed, colonize distant tissues. last step, establishment macroscopic tumors at sites, is least well understood. Four isogenic mouse breast cancer cell lines (67NR, 168FARN, 4TO7, 4T1) that differ in their ability metastasize when implanted into mammary fat pad are used model steps metastasis. Only 4T1 forms lung liver metastases. Because...
Abstract Adult tissue-derived mesenchymal stem cells (MSCs) have demonstrated therapeutic efficacy in treating diseases or repairing damaged tissues through mechanisms thought to be mediated by either cell replacement secretion of paracrine factors. Characterized, self-renewing human ESCs could potentially an invariable source consistently uniform MSCs for applications. Here we describe a clinically relevant and reproducible manner generating identical batches hESC-derived MSC (hESC-MSC)...
AbstractMicroRNAs (miRNAs) are a class of small noncoding RNAs that regulate gene expression at the posttranscriptional level. Research on miRNAs has highlighted their importance in neural development, but specific functions neurally enriched remain poorly understood. We report here profile during neuronal differentiation human neuroblastoma cell line SH-SY5Y. Six were significantly upregulated induced by all-trans-retinoic acid and brain-derived neurotrophic factor. demonstrated ectopic...