Tony Kiat Hon Lim
A5016405197- Cancer Genomics and Diagnostics
- Cancer Immunotherapy and Biomarkers
- Lung Cancer Treatments and Mutations
- RNA modifications and cancer
- Immunotherapy and Immune Responses
- Ferroptosis and cancer prognosis
- Immune Cell Function and Interaction
- Immune cells in cancer
- Cancer-related molecular mechanisms research
- Radiomics and Machine Learning in Medical Imaging
- Genetic factors in colorectal cancer
- Hepatocellular Carcinoma Treatment and Prognosis
- Lung Cancer Research Studies
- Pancreatic and Hepatic Oncology Research
- Colorectal Cancer Treatments and Studies
- T-cell and B-cell Immunology
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Liver Disease Diagnosis and Treatment
- Gastric Cancer Management and Outcomes
- Lung Cancer Diagnosis and Treatment
- Single-cell and spatial transcriptomics
- Hepatitis B Virus Studies
- Cancer, Hypoxia, and Metabolism
- Cancer Cells and Metastasis
- Viral-associated cancers and disorders
Duke-NUS Medical School
2016-2025
Singapore General Hospital
2016-2025
National University of Singapore
2017-2025
University of Science and Technology of China
2025
SingHealth Duke-NUS Academic Medical Centre
2022-2024
Genome Institute of Singapore
2016-2018
Agency for Science, Technology and Research
2016-2018
Nanyang Technological University
2016
Seattle University
2015
Dendritic cells (DC) are professional antigen-presenting that orchestrate immune responses. The human DC population comprises two main functionally specialized lineages, whose origins and differentiation pathways remain incompletely defined. Here, we combine high-dimensional technologies-single-cell messenger RNA sequencing (scmRNAseq) cytometry by time-of-flight (CyTOF)-to identify blood CD123+CD33+CD45RA+ precursors (pre-DC). Pre-DC share surface markers with plasmacytoid (pDC) but have...
Background and aims Chronic inflammation induced by chronic hepatitis B virus (HBV) infection increases the risk of hepatocellular carcinoma (HCC). However, little is known about immune landscape HBV-related HCC its influence on design effective cancer immunotherapeutics. Methods We interrogated microenvironments non-viral-related using immunohistochemistry cytometry time-of-flight (CyTOF). On identifying unique subsets enriched in HCC, we further their phenotypes functions next-generation...
Bladder cancer with loss of KDM6A expression is vulnerable to inhibition EZH2.
Objectives Yttrium-90 (Y90)-radioembolisation (RE) significantly regresses locally advanced hepatocellular carcinoma and delays disease progression. The current study is designed to deeply interrogate the immunological impact of Y90-RE, which elicits a sustained therapeutic response. Design Time-of-flight mass cytometry next-generation sequencing (NGS) were used analyse immune landscapes tumour-infiltrating leucocytes (TILs), tumour tissues peripheral blood mononuclear cells (PBMCs) at...
Hypoxia is one of the central players in shaping immune context tumor microenvironment (TME). However, complex interplay between cell infiltrates within hypoxic TME HCC remains to be elucidated.We analyzed landscapes hypoxia-low and hypoxia-high regions using cytometry by time light, immunohistochemistry, transcriptomic analyses. The mechanisms immunosuppression subsets interest were further explored vitro hypoxia assays. Regulatory T cells (Tregs) a number immunosuppressive myeloid subsets,...
The clinical relevance of immune landscape intratumoural heterogeneity (immune-ITH) and its role in tumour evolution remain largely unexplored. Here, we uncover significant spatial phenotypic immune-ITH from multiple sectors decipher relationship with disease progression hepatocellular carcinomas (HCC). Immune-ITH is associated transcriptomic-ITH, mutational burden distinct microenvironments. Tumours low experience higher immunoselective pressure escape via loss heterozygosity human...
While immune checkpoint blockade (ICB) has shown promise in patients with hepatocellular carcinoma (HCC), it is associated modest response rates and immune-related adverse events (irAEs) are common. In this study, we aimed to decipher trajectories mechanisms of and/or irAEs HCC receiving anti-programmed cell death 1 (anti-PD-1) therapy.Pre- on-treatment peripheral blood samples (n = 60) obtained from 32 (Singapore cohort) were analysed by cytometry time-of-flight single-cell RNA sequencing,...
Abstract EGFR -mutant lung adenocarcinomas (LUAD) display diverse clinical trajectories and are characterized by rapid but short-lived responses to tyrosine kinase inhibitors (TKIs). Through sequencing of 79 spatially distinct regions from 16 early stage tumors, we show that despite low mutation burdens, Asian LUADs unexpectedly exhibit a complex genomic landscape with frequent whole-genome doubling, aneuploidy, high clonal diversity. Multiple truncal alterations, including TP53 mutations...
Abstract Hepatocellular carcinoma (HCC) has one of the poorest survival rates among cancers. Using multi-regional sampling nine resected HCC with different aetiologies, here we construct phylogenetic relationships these sectors, showing diverse levels genetic sharing, spanning early to late diversification. Unlike variegated pattern found in colorectal cancers, a large proportion display clear isolation-by-distance where spatially closer sectors are genetically more similar. Two...
Abstract Resistance to drug therapy is a major concern in cancer treatment. To probe clones resistant chemotherapy, the current approach conduct pooled cell analysis. However, this can yield false negative outcomes, especially when we are analyzing rare number of circulating tumor cells (CTCs) among an abundance other types. Here, develop microfluidic device that able perform high throughput, selective picking and isolation single CTC 100% purity from larger population cells. This...
Hepatocellular carcinoma (HCC) is the fifth most common cancer with high mortality, due to late diagnosis and limited treatment options. Blood miRNAs, which circulate in a highly stable, cell-free form, show promise as novel potential biomarkers for early detection of HCC. Whole miRNome profiling was performed identify deregulated miRNAs between HCC normal healthy (NH) volunteers. These were validated an independent cohort HCC, NH chronic Hepatitis B (CHB) volunteers finally 3rd comprising...