A5022940367- Hepatocellular Carcinoma Treatment and Prognosis
- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Viral-associated cancers and disorders
- Colorectal Cancer Treatments and Studies
- CAR-T cell therapy research
- Ferroptosis and cancer prognosis
- Immune cells in cancer
- RNA modifications and cancer
- Cancer Genomics and Diagnostics
- MicroRNA in disease regulation
- Cancer, Lipids, and Metabolism
- Lymphoma Diagnosis and Treatment
- Cancer Mechanisms and Therapy
- Lung Cancer Treatments and Mutations
- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- Cancer Treatment and Pharmacology
- Hepatitis B Virus Studies
- Liver Disease Diagnosis and Treatment
- Cancer Research and Treatments
- T-cell and B-cell Immunology
- Liver physiology and pathology
- Airway Management and Intubation Techniques
National Cancer Centre Singapore
2016-2025
SingHealth
2017-2025
Duke-NUS Medical School
2013-2024
Monash Health
2023
Woodlands Health Campus
2019-2022
Tan Tock Seng Hospital
2017-2021
Tessa Therapeutic (Singapore)
2018-2020
Agency for Science, Technology and Research
2007-2020
Institute of Molecular and Cell Biology
2020
Singapore Immunology Network
2020
Gastric cancer is a major gastrointestinal malignancy for which targeted therapies are emerging as treatment options. This study sought to identify the most prevalent molecular targets in gastric and elucidate systematic patterns of exclusivity co-occurrence among these targets, through comprehensive genomic analysis large panel cancers.Using high-resolution single nucleotide polymorphism arrays, copy number alterations were profiled 233 cancers (193 primary tumours, 40 cell lines) 98...
<h3>Objective</h3> The nature of the tumour-infiltrating leucocytes (TILs) is known to impact clinical outcome in carcinomas, including hepatocellular carcinoma (HCC). However, role B cells (TIBs) remains controversial. Here, we investigate TIBs and their interaction with T on HCC patient prognosis. <h3>Design</h3> Tissue samples were obtained from 112 patients Singapore, Hong Kong Zurich analysed using immunohistochemistry immunofluorescence. RNA expression <i>CD19, CD8A, IFNG</i> was...
Hepatocellular carcinoma (HCC) is a heterogeneous disease with poor prognosis and limited methods for predicting patient survival. The nature of the immune cells that infiltrate tumours known to impact clinical outcome. However, molecular events regulate this infiltration require further understanding. Here ability genes expressed in tumour microenvironment predict progression was investigated.Using quantitative PCR, expression 14 resected tissues from 57 Singaporean patients analysed....
Background and aims Chronic inflammation induced by chronic hepatitis B virus (HBV) infection increases the risk of hepatocellular carcinoma (HCC). However, little is known about immune landscape HBV-related HCC its influence on design effective cancer immunotherapeutics. Methods We interrogated microenvironments non-viral-related using immunohistochemistry cytometry time-of-flight (CyTOF). On identifying unique subsets enriched in HCC, we further their phenotypes functions next-generation...
Tumor tropism of human bone marrow-derived mesenchymal stem cells (MSC) has been exploited for the delivery therapeutic genes anticancer therapy. However, exact contribution these in tumor microenvironment remains unknown. In this study, we examined biological effect MSC on cells. The results showed that inhibited growth glioma cell lines and patient-derived primary vitro. Coadministration resulted significant reduction volume vascular density, which was not observed when injected with...
The recent development of immunotherapy as a cancer treatment has proved effective over years, but the precise dynamics between tumor microenvironment (TME), nontumor (NTME), and systemic immune system remain elusive. Here, we interrogated these compartments in hepatocellular carcinoma (HCC) using high-dimensional proteomic transcriptomic analyses. By time-of-flight mass cytometry, found that TME was enriched regulatory T cells (Tregs), tissue resident memory CD8+ (TRMs), natural killer...
As the current therapeutic strategies for human hepatocellular carcinoma (HCC) have been proven to limited effectiveness, immunotherapy becomes a compelling way tackle disease. We aim provide humanised mouse (humice) models understanding of interaction between cancer and immune system, particularly human-specific drug testing.
Hypoxia is one of the central players in shaping immune context tumor microenvironment (TME). However, complex interplay between cell infiltrates within hypoxic TME HCC remains to be elucidated.We analyzed landscapes hypoxia-low and hypoxia-high regions using cytometry by time light, immunohistochemistry, transcriptomic analyses. The mechanisms immunosuppression subsets interest were further explored vitro hypoxia assays. Regulatory T cells (Tregs) a number immunosuppressive myeloid subsets,...
The clinical relevance of immune landscape intratumoural heterogeneity (immune-ITH) and its role in tumour evolution remain largely unexplored. Here, we uncover significant spatial phenotypic immune-ITH from multiple sectors decipher relationship with disease progression hepatocellular carcinomas (HCC). Immune-ITH is associated transcriptomic-ITH, mutational burden distinct microenvironments. Tumours low experience higher immunoselective pressure escape via loss heterozygosity human...
Epstein-Barr virus-associated smooth muscle tumors (EBV-SMT) are rare lesions that occur in immunocompromised patients. Because they have not been fully characterized pathologically or at the molecular level, we studied 29 from 19 patients, largest series to date. Cases coded as EBV-SMT were identified patients consultation files and renal transplant database Singapore General Hospital. occurred adults (mean age 39 years; range, 21-57 years) predominantly affected males (12 male, 7 female)....
Mixed lymphohematopoietic chimerism can be induced in mice with bone marrow transplantation (BMT) after a nonmyeloablative preparative regimen that includes cyclophosphamide, anti-T-cell antibody therapy, and thymic i rradiation.These mixed chimeras are resistant to the induction of graft-versus-host disease (GVHD) delayed donor leukocyte infusions (DLIs), despite potent reaction convert s chimeric state full one.Based on this animal model, we initiated trial therapy HLA-matched or...
The tumor microenvironment plays a critical role in cancer development, progression, and control. molecular cellular nature of the immune influences disease outcome by altering balance suppressive versus cytotoxic responses vicinity tumor. Recent developments systems biology have improved our understanding complex interactions between tumors their immunological various human cancers. Effective surveillance host system protects against disease, but chronic inflammation "immunoediting" also...
Molecularly targeted agents with anti-angiogenic activity, including bevacizumab, have demonstrated clinical activity in patients advanced/metastatic hepatocellular carcinoma (HCC). This multicentre phase II study involving from several Asian countries sought to evaluate the safety and efficacy of bevacizumab plus capecitabine this population.Histologically proven/clinically diagnosed advanced HCC received 7.5 mg kg(-1) on day 1 800 m(-2) twice daily days 1-14 every 3 weeks as first-line...
MicroRNA-224 (miR-224) is one of the most commonly up-regulated microRNAs in hepatocellular carcinoma (HCC), which affects crucial cellular processes such as apoptosis and cell proliferation. In this study, we aim to elucidate molecular mechanism that leads overexpression miR-224 HCC. We examined transcript expression neighboring miR-452 genes on chromosome Xq28 tumor paired adjacent nontumorous tissues from 100 patients with HCC found coordinately its microRNA (miRNA) genes. This...
Introduction Hepatocellular carcinoma (HCC) is the fourth leading cause of cancer-associated mortality globally. Immune-checkpoint blockade (ICB) one systemic therapy options for HCC. However, response rates remain low, necessitating robust predictive biomarkers. In present study, we examined expression CD38, a molecule involved in immunosuppressive adenosinergic pathway, on immune cells tumor microenvironment. We then investigated association between CD38 and ICB treatment outcomes advanced...