A5059037164- Viral Infectious Diseases and Gene Expression in Insects
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- RNA modifications and cancer
- Protein purification and stability
- Neonatal Respiratory Health Research
- Anesthesia and Neurotoxicity Research
- Monoclonal and Polyclonal Antibodies Research
- Metabolomics and Mass Spectrometry Studies
- Glycosylation and Glycoproteins Research
- Immune Cell Function and Interaction
- Infant Development and Preterm Care
- Advanced Proteomics Techniques and Applications
- Birth, Development, and Health
- Microbial Metabolic Engineering and Bioproduction
- Cancer, Hypoxia, and Metabolism
- Pancreatic and Hepatic Oncology Research
- CAR-T cell therapy research
- Cancer, Lipids, and Metabolism
- Neuroendocrine Tumor Research Advances
- Cancer-related Molecular Pathways
- CRISPR and Genetic Engineering
- Mass Spectrometry Techniques and Applications
- Gene Regulatory Network Analysis
- Phagocytosis and Immune Regulation
Agency for Science, Technology and Research
2015-2024
Bioprocessing Technology Institute
2015-2024
Construction Industry Training Board
2024
Star Technology and Research (United States)
2023
National University of Singapore
2012
Imperial College London
2006-2011
Mount Sinai Beth Israel
2002
GlaxoSmithKline (United Kingdom)
2002
Chang Gung Memorial Hospital
1994-1999
Patients with pancreatic ductal adenocarcinoma (PDAC) face a clinically intractable disease poor survival rates, attributed to exceptionally high levels of metastasis. Epithelial-to-mesenchymal transition (EMT) is pronounced at inflammatory foci within the tumor; however, immunological mechanisms promoting tumor dissemination remain unclear. It well established that tumors exhibit Warburg effect, preferential use glycolysis for energy production, even in presence oxygen, support rapid...
Abstract The increasing demand for recombinant therapeutic proteins highlights the need to constantly improve efficiency and yield of these biopharmaceutical products from mammalian cells, which is fully achievable only through proper understanding cellular functioning. Towards this end, current study exploited a combined metabolomics in silico modeling approach gain deeper insight into mechanisms Chinese hamster ovary (CHO) fed‐batch cultures. Initially, extracellular intracellular...
Effective development of host cells for therapeutic protein production is hampered by the poor characterization cellular transfection. Here, we employed a multi-omics-based systems biotechnology approach to elucidate genotypic and phenotypic differences between wild-type recombinant antibody-producing Chinese hamster ovary (CHO) cell line. At genomic level, observed extensive rearrangements in specific targeted loci linked transgene integration sites. Transcriptional re-wiring DNA damage...
Cell state transitions control the functional behavior of cancer cells. Epithelial-to-mesenchymal transition (EMT) confers stem cell-like properties, enhanced tumorigenicity and drug resistance to tumor cells, while mesenchymal-epithelial (MET) reverses these phenotypes. Using high-throughput chemical library screens, retinoids are found be potent promoters MET that inhibit in basal-like breast cancer. defined by reprogramming lipid metabolism. Retinoids bind cognate nuclear receptors, which...
Abstract The selection of suitable mammalian cell lines with high specific productivities is a crucial aspect large‐scale recombinant protein production. This study utilizes metabolomics approach to elucidate the key characteristics Chinese hamster ovary (CHO) cells monoclonal antibody ( q mAb ). Liquid chromatography‐mass spectrometry (LC‐MS)‐based intracellular metabolite profiles eight single clones and low were obtained at mid‐exponential phase during shake flask batch cultures....
There are considerable challenges in directly targeting the mutant p53 protein, given large heterogeneity of mutations clinic. An alternative approach is to exploit altered fitness cells imposed by loss-of-wild-type p53. Here we identify niclosamide through a HTS screen for compounds selectively killing p53-deficient cells. Niclosamide impairs growth and patient-derived ovarian xenografts. Metabolome profiling reveals that induces mitochondrial uncoupling, which renders susceptible...
Abstract A metabolomics‐based approach was used to time profile extracellular metabolites in duplicate fed‐batch bioreactor cultures of recombinant Chinese Hamster Ovary (CHO) cells producing monoclonal IgG antibody. Culture medium collected and analysed using a high‐performance liquid chromatography (HPLC) system tandem with an LTQ‐Orbitrap mass spectrometer. An in‐house software developed pre‐process the LC/MS data terms filtering peak detection. This followed by principal component...
Abstract Silent biosynthetic gene clusters represent a potentially rich source of new bioactive compounds. We report the discovery, characterization, and biosynthesis novel doubly glycosylated 24‐membered polyene macrolactam from silent cluster in Streptomyces roseosporus by using CRISPR‐Cas9 activation strategy. Structural characterization this polyketide, named auroramycin, revealed rare isobutyrylmalonyl extender unit unique pair amino sugars. Relative absolute stereochemistry were...
Abstract Chinese hamster ovary (CHO) cells are the most prevalent mammalian cell factories for producing recombinant therapeutic proteins due to their ability synthesize human‐like post‐translational modifications and ease of maintenance in suspension cultures. Currently, a wide variety CHO host lines has been developed; substantial differences exist phenotypes even when transfected with same target vector. However, relatively less is known about influence inherited genetic heterogeneity on...
Abstract Mammalian cells are generally considered to be unable utilize polysaccharides for cell growth because the phospholipid bilayer in membrane has very low permeability sugars. With recent discovery of only known animal disaccharide transporter, a sucrose we potential use as energy source, that can impact biopharmaceutical manufacturing by potentially increasing carbohydrate loading culture medium and decreasing lactate accumulation. In this study, found mammalian maltose absence...
The ability to tolerate organic solvents is essential for bacteria producing these chemicals with high titer, yield, and productivity. As exemplified by 2-PE, bioproduction of 2-PE represents a promising alternative chemical synthesis plant extraction approaches, but its toxicity hinders successful large-scale microbial production.
Abstract Background The compromised gut microbiome that results from C-section birth has been hypothesized as a risk factor for the development of non-communicable diseases (NCD). In double-blind randomized controlled study, 153 infants born by elective received an infant formula supplemented with either synbiotic, prebiotics, or unsupplemented until 4 months old. Vaginally were included reference group. Stool samples collected day 3 till week 22. Multi-omics deployed to investigate impact...
Cottonseed meal (CSM) is a good source of dietary proteins but unsuitable for human consumption due to its gossypol content. To unlock potential, we developed protein extraction process with removal treatment generate CSM isolate (CSMPI) ultra-low This successfully reduced the free and total content 4.8 ppm 147.2 ppm, respectively, far below US FDA limit. In addition, functional characterisation CSMPI revealed better oil absorption capacity water solubility than pea isolate. Proteome...