A5041112048- CRISPR and Genetic Engineering
- RNA regulation and disease
- RNA Research and Splicing
- Pluripotent Stem Cells Research
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Advanced biosensing and bioanalysis techniques
- Genetics, Bioinformatics, and Biomedical Research
- Tea Polyphenols and Effects
- Cytomegalovirus and herpesvirus research
- SARS-CoV-2 and COVID-19 Research
- Cancer-related molecular mechanisms research
- Biosensors and Analytical Detection
- SARS-CoV-2 detection and testing
- MicroRNA in disease regulation
- Tannin, Tannase and Anticancer Activities
- Phytochemicals and Antioxidant Activities
- Bacterial Genetics and Biotechnology
- RNA Interference and Gene Delivery
- Viral Infectious Diseases and Gene Expression in Insects
- Gene Regulatory Network Analysis
- Plant Gene Expression Analysis
- Microbial Metabolic Engineering and Bioproduction
- Molecular Biology Techniques and Applications
- Biochemical and biochemical processes
Fujian Agriculture and Forestry University
2020-2025
Nanyang Technological University
2014-2024
Genome Institute of Singapore
2014-2024
Agency for Science, Technology and Research
2014-2024
Yangzhou University
2024
Chongqing University
2023
Sichuan Agricultural University
2022
Shanghai Ocean University
2021
Stanford University
2007-2017
California Institute of Technology
2004
Extensive testing is essential to break the transmission of SARS-CoV-2, which causes ongoing COVID-19 pandemic. Here, we present a CRISPR-based diagnostic assay that robust viral genome mutations and temperature, produces results fast, can be applied directly on nasopharyngeal (NP) specimens without RNA purification, incorporates human internal control within same reaction. Specifically, show use an engineered AsCas12a enzyme enables detection wildtype mutated SARS-CoV-2 allows us perform...
Li et al . (Research Articles, 1 July 2011, p. 53; published online 19 May 2011) reported widespread differences between the RNA and DNA sequences of same human cells, including all 12 possible mismatch types. Before accepting such a fundamental claim, deeper analysis sequencing data is required to discern true from potential artifacts.
The prokaryotic type II CRISPR/Cas9 system has been adapted to perform targeted genome editing in cells and model organisms. Here, we describe gene deletion replacement human via the using two guide RNAs. effectively generated deletions of varied length, regardless transcriptional status target gene. It is notable that RNAs resulted formation correct junctions at high efficiency. Moreover, presence a homology repair donor, could precise replacement. Our results illustrate can be used...
Embryonic stem cells (ESCs) and induced pluripotent (iPSCs) efficiently generate all embryonic cell lineages but rarely extraembryonic types. We found that microRNA miR-34a deficiency expands the developmental potential of mouse cells, yielding both strongly inducing MuERV-L (MERVL) endogenous retroviruses, similar to what is seen with features totipotent two-cell blastomeres. restricts acquisition expanded fate in it represses MERVL expression through transcriptional regulation, at least...
The Xenopus embryo has provided key insights into fate specification, the cell cycle, and other fundamental developmental cellular processes, yet a comprehensive understanding of its transcriptome is lacking. Here, we used paired end RNA sequencing (RNA-seq) to explore tropicalis in 23 distinct stages. We determined expression levels all genes annotated RefSeq Ensembl showed for first time on genome-wide scale that, despite general state transcriptional silence earliest stages development,...
While CRISPR-Cas systems hold tremendous potential for engineering the human genome, it is unclear how well each system performs against one another in both non-homologous end joining (NHEJ)-mediated and homology-directed repair (HDR)-mediated genome editing.
The availability of different host chassis will greatly expand the range applications in synthetic biology. Members Acetobacteraceae family Gram-negative bacteria form an attractive class nonmodel microorganisms that can be exploited to produce industrial chemicals, food and beverage, biomaterials. One such biomaterial is bacterial cellulose, which a strong ultrapure natural polymer used tissue engineering scaffolds, wound dressings, electronics, additives, other products. However, despite...
Due to increased global warming and climate change, drought has become a serious threat horticultural crop cultivation management. The purpose of this study was investigate the effect spermine (Spm) pretreatment on metabolic alterations polyamine (PAs), γ-aminobutyric acid (GABA), proline (Pro), nitrogen associated with tolerance in creeping bentgrass (Agrostis stolonifera). results showed that could be significantly improved by Spm pretreatment, as demonstrated maintenance less chlorophyll...
Divisions of polarised blastomeres that allocate polar cells to outer and apolar inner positions initiate the first cell fate decision in mouse embryo. Subsequently, differentiate into trophectoderm while retain pluripotency become mass (ICM) blastocyst. Elimination zygotic expression trophectoderm-specific transcription factor Cdx2 leads defects maintenance blastocyst cavity, suggesting it participates only late stage formation. However, we now find embryos also have a maternally provided...
A cyclical control circuit composed of four master regulators drives the Caulobacter cell cycle. We report that SciP, a helix-turn-helix transcription factor, is an essential component this circuit. SciP cycle-controlled and co-conserved with global cycle regulator CtrA in α-proteobacteria. expressed late accumulates preferentially daughter swarmer cell. At least 58 genes, including many flagellar chemotaxis are regulated by type 1 incoherent feedforward motif which activates sciP, followed...
Landmark events occur in a coordinated manner during pre-implantation development of the mammalian embryo, yet regulatory network that orchestrates these remains largely unknown. Here, we present first systematic investigation mouse embryos using morpholino-mediated gene knockdowns key embryonic stem cell (ESC) factors followed by detailed transcriptome analysis pooled embryos, single and individual blastomeres. We delineated regulons Oct4, Sall4, Nanog identified set metabolism-...
Personal exome and genome sequencing provides access to loss-of-function rare deleterious alleles whose interpretation is expected provide insight into individual disease burden. However, for each allele, accurate of its effect will depend on both penetrance the trait's expressivity. In this regard, an important factor that can modify a pathogenic coding allele level expression; which itself characteristically changes across tissues. To better inform degree be modified by expression multiple...
Abstract Mammalian host cell lines are the preferred expression systems for manufacture of complex therapeutics and recombinant proteins. However, most utilized mammalian systems, namely Chinese hamster ovary (CHO), Sp2/0 NS0 mouse myeloma cells, can produce glycoproteins with non-human glycans that may potentially illicit immunogenic responses. Hence, we developed a fully human system based on HEK293 cells stable high titer production proteins by first knocking out GLUL (encoding glutamine...
Catechins are crucial in determining the flavour and health benefits of tea, but it remains unclear that how light intensity regulates catechins biosynthesis. Therefore, we cultivated tea plants a phytotron to elucidate response mechanism biosynthesis changes.In 250 μmol·m- 2·s- 1 treatment, contents epigallocatechin, epigallocatechin gallate total were increased by 98.94, 14.5 13.0% respectively, compared with those 550 treatment. Meanwhile, photosynthetic capacity was enhanced including...
The raging COVID-19 pandemic has created an unprecedented demand for frequent and widespread testing to limit viral transmission. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) emerged as a promising diagnostic platform rapid detection of SARS-CoV-2, in part because it can be performed with simple instrumentation. However, methods frequently yield spurious amplicons even the absence template. Consequently, RT-LAMP assays produce false positive results when they are...
Selection and utilization of salt-tolerant crops are essential strategies for mitigating salinity damage to crop productivity with increasing soil salinization worldwide. This study was conducted identify white clover (Trifolium repens) genotypes among 37 materials based on a comprehensive evaluation five physiological parameters, namely, chlorophyll (Chl) content, photochemical efficiency PS II (Fv/Fm), performance index an absorption basis (PIABS), leaf relative water content (RWC),...
Epigallocatechin gallate (EGCG) imparts unique health benefits and flavour to tea. Photosynthesis plays a crucial role in modulating secondary metabolite production plants, this study investigated its impact on the biosynthesis of EGCG tea plants under different ecological conditions. Enhanced photosynthetic activity increased content, total esterified catechins (TEC), (TC) responded synchronously changes factors. The capacity content fit surface model equations (Extreme 2D Polynomial 2D)...