A5048478659- Cytokine Signaling Pathways and Interactions
- Neurogenesis and neuroplasticity mechanisms
- Chronic Myeloid Leukemia Treatments
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Pluripotent Stem Cells Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Epigenetics and DNA Methylation
- FOXO transcription factor regulation
- Genetics, Aging, and Longevity in Model Organisms
- CRISPR and Genetic Engineering
- Single-cell and spatial transcriptomics
- Retinoids in leukemia and cellular processes
- Acute Myeloid Leukemia Research
- Genomics and Chromatin Dynamics
- Reproductive System and Pregnancy
- CAR-T cell therapy research
- Kruppel-like factors research
- Plant Molecular Biology Research
- Estrogen and related hormone effects
- Atherosclerosis and Cardiovascular Diseases
- RNA regulation and disease
- Cytomegalovirus and herpesvirus research
- T-cell and Retrovirus Studies
- Autophagy in Disease and Therapy
- Aging and Gerontology Research
Stanford University
2010-2024
Brigham and Women's Hospital
2007-2011
Harvard University
2007-2011
Howard Hughes Medical Institute
2007-2008
Instituto Benjamin Constant
2007
Woman's Hospital
2007
Dana-Farber Cancer Institute
2007
Lysosomes keep neuronal stem cells young An important consequence of aging is loss regenerative capacity in cells, particularly those the nervous system. Leeman et al. isolated quiescent and activated from mice compared their transcriptomes. The findings emphasize role large lysosomes which aggregated proteins accumulate. Treatments that stimulated lysosomal function allowed aged to clear protein aggregates restored cells' ability be activated. Such restoration cell might alleviate...
Neural stem cells (NSCs) in the adult mammalian brain serve as a reservoir for generation of new neurons, oligodendrocytes, and astrocytes. Here, we use single-cell RNA sequencing to characterize NSC populations examine molecular identities heterogeneity vivo populations. We find that lineage exist on continuum through processes activation differentiation. Interestingly, rare intermediate states with distinct profiles can be identified experimentally validated, our analysis identifies...
Ageing impairs the ability of neural stem cells (NSCs) to transition from quiescence proliferation in adult mammalian brain. Functional decline NSCs results decreased production new neurons and defective regeneration following injury during ageing
Abstract Aging impairs the ability of neural stem cells to transition from quiescence activation (proliferation) in adult mammalian brain. Neural cell (NSC) functional decline results decreased production new neurons and defective regeneration upon injury during aging 1–9 , this is exacerbated Alzheimer’s disease 10 . Many genes are upregulated with age NSCs 3, 11–13 knockout some these boosts old NSC rejuvenates aspects brain function 14–18 But systematic testing – more generally has not...
Glioblastoma (GBM) is an aggressive, age-associated malignant glioma that contains populations of cancer stem cells. These cells (GSCs) evade therapeutic interventions and repopulate tumors due to their existence in a slowly cycling quiescent state. Although aging well known increase initiation, the extent which mechanisms supporting GSC tumorigenicity are related physiological remains unknown. Here, we investigate transcriptional by Forkhead Box O3 (FOXO3), regulator promotes healthy aging,...
Background: CRISPR-based genetic medicines offer a promising “one and done” approach to improve cardiovascular health by lowering LDL-C. We performed comparative analysis of different gene editing approaches targeting PCSK9 for potent safe Based on our findings, we engineered two investigational products using novel CRISPR-CasX platform: editor an epigenetic editor. demonstrated the efficacy safety these agents in vivo, including non-human primates (NHPs). Methods: CasX-based molecules....
Abstract Suppressing spurious cryptic transcription by a repressive intragenic chromatin state featuring trimethylated lysine 36 on histone H3 (H3K36me3) and DNA methylation is critical for maintaining self-renewal capacity in mouse embryonic stem cells. In yeast nematodes, such elevated with age, reducing the levels of age-associated extends lifespan. Whether also increased during mammalian aging unknown. We show first time an elevation several cell populations, including murine...