Benson M. George
A5060533219- Single-cell and spatial transcriptomics
- Hematopoietic Stem Cell Transplantation
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- RNA modifications and cancer
- Immune cells in cancer
- RNA and protein synthesis mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Glycosylation and Glycoproteins Research
- Acute Myeloid Leukemia Research
- Neurogenesis and neuroplasticity mechanisms
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Phagocytosis and Immune Regulation
- Epigenetics and DNA Methylation
- Nausea and vomiting management
- Anesthesia and Pain Management
- Spine and Intervertebral Disc Pathology
- Cell Image Analysis Techniques
- Bone health and osteoporosis research
- Erythrocyte Function and Pathophysiology
- Dental Anxiety and Anesthesia Techniques
- Monoclonal and Polyclonal Antibodies Research
- Pluripotent Stem Cells Research
- Chromosomal and Genetic Variations
Dana-Farber Cancer Institute
2024-2025
Boston Children's Hospital
2023-2025
Boston Children's Museum
2025
Harvard University
2023-2024
Stanford University
2015-2023
Brigham and Women's Hospital
2022-2023
Boston University
2023
California Institute for Regenerative Medicine
2016-2022
Institute for Stem Cell Biology and Regenerative Medicine
2019-2020
Maimonides Medical Center
2016-2019
To define the cellular composition and architecture of cutaneous squamous cell carcinoma (cSCC), we combined single-cell RNA sequencing with spatial transcriptomics multiplexed ion beam imaging from a series human cSCCs matched normal skin. cSCC exhibited four tumor subpopulations, three recapitulating epidermal states, tumor-specific keratinocyte (TSK) population unique to cancer, which localized fibrovascular niche. Integration data mapped ligand-receptor networks specific types, revealing...
Aneuploidy decreases cellular fitness, yet it is also associated with cancer, a disease of enhanced proliferative capacity. To investigate one mechanism by which aneuploidy could contribute to tumorigenesis, we examined the effects on genomic stability. We analyzed 13 budding yeast strains that carry extra copies single chromosomes and found all aneuploid exhibited or more forms instability. Most displayed increased chromosome loss mitotic recombination, as well defective DNA damage repair....
Interleukin-2 (IL-2) is a cytokine required for effector T cell expansion, survival, and function, especially engineered cells in adoptive immunotherapy, but its pleiotropy leads to simultaneous stimulation suppression of immune responses as well systemic toxicity, limiting therapeutic use. We IL-2 cytokine-receptor orthogonal (ortho) pairs that interact with one another, transmitting native signals, do not their natural receptor counterparts. Introduction orthoIL-2Rβ into enabled the...
Significance Cancer immunotherapy is a promising therapeutic intervention. However, complete and durable responses are only seen in fraction of patients who have cancer. Although cells the myeloid lineage frequently infiltrate tumors limit success, currently approved immunotherapies primarily target tumor-infiltrating T natural killer lymphocytes. The inhibitory receptor signal regulatory protein-α (SIRPα) represents myeloid-specific immune checkpoint that engages “don’t eat me” CD47. Here,...
Abstract The diversity of cell types is a challenge for quantifying aging and its reversal. Here we develop ‘aging clocks’ based on single-cell transcriptomics to characterize cell-type-specific rejuvenation. We generated transcriptomes from the subventricular zone neurogenic region 28 mice, tiling ages young old. trained single-cell-based regression models predict chronological age biological (neural stem proliferation capacity). These clocks are generalizable independent cohorts other...
Targeted protein degradation is an emerging strategy for the elimination of classically undruggable proteins. Here, to expand landscape targetable substrates, we designed degraders that achieve substrate selectivity via recognition a discrete peptide and glycan motif cell-type antigen-driven cell-surface binding. We applied this approach mucins, O-glycosylated proteins drive cancer progression through biophysical immunological mechanisms. Engineering bacterial mucin-selective protease...
Significance This is the first in-depth profiling of pancreatic neuroendocrine tumors (PanNETs), to our knowledge, that illuminates fundamental biological processes for this class tumors. Beginning with index case and confirmed additional patient tumors, we showed dependence on paracrine signaling through hepatocyte growth factor (HGF)/receptor tyrosine kinase MET axis. We created a novel cell line derived from well-differentiated PanNET autocrine HGF/MET signaling. also identified...
Glycans modify protein, lipid, and even RNA molecules to form the regulatory outer coat on cells called glycocalyx. The changes in glycosylation have been linked initiation progression of many diseases. Thus, while significance is well established, a lack accessible methods characterize glycans has hindered ability understand their biological functions. Mass spectrometry (MS)-based generally at core most glycan profiling efforts; however, modern data-independent acquisition (DIA), which...
The balance of hematopoietic stem cell (HSC) self-renewal and differentiation is critical for a healthy blood supply; imbalances underlie hematological diseases. importance HSCs their progenitors have led to extensive characterization at genomic transcriptomic levels. However, the proteomics hematopoiesis remains incompletely understood. Here we report resource from mass spectrometry mouse young adult old HSCs, multipotent oligopotent progenitors; 12 types in total. We validated differential...
The Tabula Muris Consortium We have created a compendium of single cell transcriptome data from the model organism Mus musculus comprising more than 100,000 cells 20 organs and tissues. These represent new resource for biology, revealing gene expression in poorly characterized populations allowing direct controlled comparison types shared between tissues, such as T-lymphocytes endothelial distinct anatomical locations. Two technical approaches were used most tissues: one approach,...
Key Points Upon injury, the mesothelium recruits neutrophils to peritoneal space, which contributes adhesion formation. Neutrophil recruitment and macrophage-depletion kinetics in adhesions differ from normal innate response.
Abstract Chemical tools enable precise characterization of many biopolymers, including glycoconjugates. Metabolic chemical reporters enabled the discovery glycoRNAs, however they have certain limitations due requirement having living cells to incorporate modified sugar. Here we develop a periodate oxidation and aldehyde ligation method detect characterize native sialoglycoRNAs, termed rPAL. With optimized RNA biochemistry enhance recovery analysis small RNAs, show rPAL is at least an order...