A5028873839- Wound Healing and Treatments
- Dermatologic Treatments and Research
- Mesenchymal stem cell research
- Surgical Sutures and Adhesives
- Cancer Cells and Metastasis
- Cellular Mechanics and Interactions
- Spine and Intervertebral Disc Pathology
- Neonatal Respiratory Health Research
- Intestinal and Peritoneal Adhesions
- Myofascial pain diagnosis and treatment
- Diabetic Foot Ulcer Assessment and Management
- Body Contouring and Surgery
- Burn Injury Management and Outcomes
- Extracellular vesicles in disease
- Skin and Cellular Biology Research
- Pain Management and Treatment
- Pressure Ulcer Prevention and Management
- Tendon Structure and Treatment
- Hair Growth and Disorders
- Exercise and Physiological Responses
- Hippo pathway signaling and YAP/TAZ
- Thyroid and Parathyroid Surgery
- Connexins and lens biology
- Hematopoietic Stem Cell Transplantation
- Planarian Biology and Electrostimulation
Helmholtz Zentrum München
2018-2025
Capital Medical University
2025
German Center for Lung Research
2018-2022
The refractory diabetic wound has remained a worldwide challenge as one of the major health problems. impaired angiogenesis phase during healing partly contributes to pathological process. MicroRNA (miRNA) is an essential regulator gene expression in crucial biological processes and promising nucleic acid drug therapeutic fields wound. However, miRNA therapies have limitations due lacking effective delivery system. In present study, we found significant reduction miR-31-5p full-thickness...
Abstract Extravasation of monocytes into tissue and to the site injury is a fundamental immunological process, which requires rapid responses via post translational modifications (PTM) proteins. Protein arginine methyltransferase 7 (PRMT7) an epigenetic factor that has capacity mono-methylate histones on residues. Here we show in chronic obstructive pulmonary disease (COPD) patients, PRMT7 expression elevated lung localized macrophages. In mouse models COPD, fibrosis skin injury, reduced...
Peritoneal adhesions are fibrous tissues that tether organs to one another or the peritoneal wall and a major cause of postsurgical infectious morbidity. The primary molecular chain events leading initiation has been elusive, chiefly due lack an identifiable cell origin. Using clonal analysis lineage tracing, we have identified injured surface mesothelium expressing podoplanin (PDPN) mesothelin (MSLN) as instigator after surgery in mice. We demonstrate anti-MSLN antibody diminished adhesion...
Abstract Scars are more severe when the subcutaneous fascia beneath dermis is injured upon surgical or traumatic wounding. Here, we present a detailed analysis of cell mobilisation by using deep tissue intravital live imaging acute wounds, fibroblast lineage-specific transgenic mice, and skin-fascia explants (scar-like in dish – SCAD). We observe that injury triggers swarming-like collective migration fibroblasts progressively contracts skin form scars. Swarming exclusive to fibroblasts,...
Internal organs heal injuries with new connective tissue, but the cellular and molecular events of this process remain obscure. By tagging extracellular matrix around mesothelium lining in mouse peritoneum, liver cecum, here we show that preexisting was transferred across into wounds various injury models. Using proteomics, genetic lineage-tracing selective juxtaposed organs, found tissue origin for likely dictated scarring or regeneration healing tissue. Single-cell RNA sequencing chemical...
Optimal tissue recovery and organismal survival are achieved by spatiotemporal tuning of inflammation, contraction scar formation1. Here we identify a multipotent fibroblast progenitor marked CD201 expression in the fascia, deepest connective layer skin. Using skin injury models mice, single-cell transcriptomics genetic lineage tracing, ablation gene deletion models, demonstrate that CD201+ progenitors control pace wound healing generating multiple specialized cell types, from...
Abstract Surgical adhesions are bands of scar tissues that abnormally conjoin organ surfaces. Adhesions a major cause post-operative and dialysis-related complications, yet their patho-mechanism remains elusive, prevention agents in clinical trials have thus far failed to achieve efficacy. Here, we uncover the adhesion initiation mechanism by coating beads with human mesothelial cells normally line surfaces, viewing them under stimuli. We document expansive membrane protrusions from...
Abstract The origins of wound myofibroblasts and scar tissue remains unclear, but it is assumed to involve conversion adipocytes into myofibroblasts. Here, we directly explore the potential plasticity fibroblasts after skin injury. Using genetic lineage tracing live imaging in explants wounded animals, observe that injury induces a transient migratory state with vastly distinct cell migration patterns behaviours from fibroblasts. Furthermore, adipocytes, do not contribute formation remain...
OPINION article Front. Cell Dev. Biol., 22 October 2018 | https://doi.org/10.3389/fcell.2018.00133
Key Points Upon injury, the mesothelium recruits neutrophils to peritoneal space, which contributes adhesion formation. Neutrophil recruitment and macrophage-depletion kinetics in adhesions differ from normal innate response.
Abstract Lung fibrosis development utilizes alveolar macrophages, with mechanisms that are incompletely understood. Here, we fate map connective tissue during mouse lung and observe disassembly transfer of macromolecules from pleuro-alveolar junctions (PAJs) into deep tissue, to activate fibroblasts fibrosis. Disassembly PAJ occurs by activating cysteine-type proteolysis on pleural mesothelium. The niche the cascade is active in patient biopsies, persists chronic models, wanes down acute...