A5003757419- DNA Repair Mechanisms
- RNA Interference and Gene Delivery
- HIV Research and Treatment
- RNA Research and Splicing
- HIV/AIDS drug development and treatment
- Immune Response and Inflammation
- HIV-related health complications and treatments
- Cell Adhesion Molecules Research
- RNA and protein synthesis mechanisms
- HIV/AIDS Research and Interventions
- Peptidase Inhibition and Analysis
- Wound Healing and Treatments
- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- Cytokine Signaling Pathways and Interactions
- 3D Printing in Biomedical Research
- Wireless Power Transfer Systems
- Seismic Imaging and Inversion Techniques
- Fungal and yeast genetics research
- Macrophage Migration Inhibitory Factor
- Effects and risks of endocrine disrupting chemicals
- Cancer Mechanisms and Therapy
- Adenosine and Purinergic Signaling
- NMR spectroscopy and applications
Guangzhou Institutes of Biomedicine and Health
2024
Chinese Academy of Sciences
2024
University of Arizona
2024
The Francis Crick Institute
2020-2023
Zhejiang University
2009-2021
Helmholtz Zentrum München
2020-2021
Ministry of Education of the People's Republic of China
2009-2020
China University of Mining and Technology
2020
First Affiliated Hospital of Bengbu Medical College
2019
Banner - University Medical Center Tucson
2014
Translating ribosomes that slow excessively incur collisions with trailing ribosomes. Persistent are detected by ZNF598, a ubiquitin ligase ubiquitinates sites on the ribosomal 40S subunit to initiate pathways of mRNA and protein quality control. The collided ribosome complex must be disassembled downstream control, but mechanistic basis disassembly is unclear. Here, we reconstitute polysome in mammalian cell-free system. widely conserved ASC-1 (ASCC) containing ASCC3 helicase disassembles...
Abstract Scars are more severe when the subcutaneous fascia beneath dermis is injured upon surgical or traumatic wounding. Here, we present a detailed analysis of cell mobilisation by using deep tissue intravital live imaging acute wounds, fibroblast lineage-specific transgenic mice, and skin-fascia explants (scar-like in dish – SCAD). We observe that injury triggers swarming-like collective migration fibroblasts progressively contracts skin form scars. Swarming exclusive to fibroblasts,...
The cyclic GMP-AMP synthase-stimulator of interferon genes (cGAS-STING) pathway senses cytosolic DNA and induces interferon-stimulated (ISGs) to activate the innate immune system. Here, we report unexpected discovery that cGAS also dysfunctional protein production. Purified ribosomes interact directly with stimulate its DNA-dependent activity in vitro. Disruption ribosome-associated quality control (RQC) pathway, which detects resolves ribosome collision during translation, results...
RNA polymerase II (RNAPII) transcription involves initiation from a promoter, transcriptional elongation through the gene, and termination in terminator region. In bacteria, terminators often contain specific DNA elements provoking dissociation, but RNAPII is thought to be driven entirely by protein co-factors. We used biochemical reconstitution, single-molecule studies, genome-wide analysis yeast study termination. Transcription into natural pure results spontaneous at sequences containing...
The Bloom syndrome gene product, BLM, is a member of the highly conserved RecQ family. An emerging concept BLM helicase collaborates with homologous recombination (HR) machinery to help avoid undesirable HR events and achieve high degree fidelity during reaction. However, exactly how such coordination occurs in vivo poorly understood. Here, we identified protein termed SPIDR (scaffolding involved DNA repair) as link between machinery. independently interacts RAD51 promotes formation...
It has been reported that hyaluronic acid (HA) with a 35 kDa molecular weight (HA35) acts biologically to protect tissue from injury, but its biological properties are not yet fully characterized. This study aimed evaluate the cellular effects and biodistribution of HA35 compared HA 1600 (HA1600). We assessed HA1600 on cell migration, NO ROS generation, gene expression in cultured macrophages, microglia, lymphocytes. was separately radiolabeled
The PSO4 core complex is composed of PSO4/PRP19/SNEV, CDC5L, PLRG1, and BCAS2/SPF27. Besides its well defined functions in pre-mRNA splicing, the has been shown recently to participate DNA damage response. However, specific role for response pathways still not clear. Here we show that both BCAS2 subunits directly interact colocalize with replication protein A (RPA). Depletion or impairs recruitment ATR-interacting (ATRIP) sites compromises CHK1 activation RPA2 phosphorylation. Moreover,...
Significance A timely and appropriate response to genotoxic stress is essential for maintaining genome stability preventing tumorigenesis. Here, we have established a previously unidentified role of the protein kinase ATM in controlling extent DNA end resection during homology-directed repair. Specifically, elicits sequential posttranslational modifications key factor CtIP thereby fine-tunes its activity at DSB sites. Our findings highlight multistep roles master orchestrating damage responses.
Curing HIV-1 infection has remained elusive because of low and fluctuating drug levels arising from poor absorption, the development viral reservoirs sanctuary sites, toxicity, patient nonadherence. The present study addresses issue insufficient exposure in macrophages. Viral reservoir sites such as macrophages are believed to be responsible for rebound effect observed upon discontinuation anti-HIV therapy. In our proposed model, a can covalently attached nanocarrier order facilitate...
Penetration of epithelial cells represents the rate-determining step for absorption many drugs and pharmaceutical macromolecules such as proteins nucleic acid therapeutics. While potential using cell-penetrating peptides (CPPs) to facilitate has been increasingly recognized, mechanism cell penetration uptake into certain have recently called question due methodological artifacts. Therefore, objective this study was quantitatively assess ability RI-Tat-9, a proteolytically stable CPP,...
Current anti-AIDS therapeutic agents and treatment regimens can provide a dramatically improved quality of life for HIV-positive people, many whom have no detectable viral load prolonged periods time. Despite this, curing AIDS remains an elusive goal, partially due to the occurrence drug resistance. Since development resistance is linked to, among other things, fluctuating levels, our long-term goal has been develop nanotechnology-based delivery systems that improve therapy by more precisely...
Wireline logging plays a critical role in coalbed methane exploration. However, the lack of crucial log data, such as neutron and sonic logs, makes exploration difficult. In this paper, we propose principal component regression model incorporating multiscale wavelet analysis, histogram calibration, multivariate to reconstruct essential logs from conventional (i.e., density, resistivity, gamma ray, spontaneous potential, caliper logs). Our proposed does not need core-related correlation,...
CD73, an ectoenzyme responsible for adenosine production, is often elevated in immuno-suppressive tumor environments. Inhibition of CD73 activity holds great promise as a therapeutic strategy CD73-expressing cancers. In this study, we have developed anti-human antibody cocktail, HB0045. HB0045 1:1 mixture two humanized monoclonal IgG1 antibodies (mAbs), HB0038 and HB0039. The cocktail not only harnesses the advantages its parental mAbs enzyme inhibition but also shows significantly greater...
Phthalic acid esters (PAEs), which are human endocrine disruptors, can lead to sperm production atrophy and decreased count poisonous the embryo active as anti-androgens. Many studies have been conducted determine PAE levels in drugs their corresponding plastic packaging; however, there is no systematic research on transfer of from packaging drugs. In this study, a simple convenient method using GC-MS was developed for simultaneous determination 16 PAEs. n-Hexane used extract PAEs samples,...
For quadruped robot, permanent-magnet (PM) machines are competitive due to their promising high torque density. In this paper, four PM motors with fractional-slot concentrated windings (FSCW) designed and compared. They the same design specifications but different rotor topologies, including surface-mounted (SPM), flat-shape interior (F-IPM), V-shape (V-IPM) consequent-pole (CP). Electromagnetic performances of these analyzed using finite element method (FEM) main concern comparison is...