A5017931611- SARS-CoV-2 and COVID-19 Research
- Monoclonal and Polyclonal Antibodies Research
- Cancer-related molecular mechanisms research
- Ubiquitin and proteasome pathways
- Bacteriophages and microbial interactions
- Peptidase Inhibition and Analysis
- Viral gastroenteritis research and epidemiology
- vaccines and immunoinformatics approaches
- RNA modifications and cancer
- Glycosylation and Glycoproteins Research
- Photosynthetic Processes and Mechanisms
- Enzyme Structure and Function
- Epigenetics and DNA Methylation
- interferon and immune responses
- Prenatal Screening and Diagnostics
- Antimicrobial Peptides and Activities
- Virus-based gene therapy research
- Advanced Biosensing Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- SARS-CoV-2 detection and testing
- DNA Repair Mechanisms
- Viral Infections and Immunology Research
- Advanced Glycation End Products research
- Hematopoietic Stem Cell Transplantation
- Urological Disorders and Treatments
Changsha Hospital for Maternal and Child Health Care
2005-2025
Guangzhou Institutes of Biomedicine and Health
2020-2025
Chinese Academy of Sciences
2020-2025
State Key Laboratory of Respiratory Disease
2022-2025
Hunan Normal University
2023-2025
University of Hong Kong
2024-2025
Guangzhou Medical University
2023-2025
Jinan University
2019-2024
Institute of Laboratory Animal Science
2019-2024
Guangzhou Regenerative Medicine and Health Guangdong Laboratory
2021-2023
We have produced a draft sequence of the rice genome for most widely cultivated subspecies in China, Oryza sativa L. ssp. indica , by whole-genome shotgun sequencing. The was 466 megabases size, with an estimated 46,022 to 55,615 genes. Functional coverage assembled sequences 92.0%. About 42.2% exact 20-nucleotide oligomer repeats, and transposons were intergenic regions between Although 80.6% predicted Arabidopsis thaliana genes had homolog rice, only 49.4% A. . large proportion no...
Abstract Population antibody response is thought to be important in selection of virus variants. We report that SARS-CoV-2 infection elicits a population immune mediated by lineage VH1-69 germline antibodies. A representative R1-32 from this was isolated. By cryo-EM, we show it targets semi-cryptic epitope the spike receptor-binding domain. Binding non-ACE2 competing results destruction, thereby inhibiting entry. On basis location, neutralization mechanism and analysis binding variants,...
Abstract Nipah virus (NiV) and related viruses form a distinct henipavirus genus within the Paramyxoviridae family. NiV continues to spillover into humans causing deadly outbreaks with increasing human-bat interaction. encodes large protein (L) phosphoprotein (P) viral RNA polymerase machinery. Their sequences show limited homologies those of non-henipavirus paramyxoviruses. We report two cryo-electron microscopy (cryo-EM) structures L-P complex, expressed purified in either its full-length...
Abstract ORC, Cdc6 and Cdt1 act together to load hexameric MCM, the motor of eukaryotic replicative helicase, into double hexamers at replication origins. Here we show that interacts with MCM subunits Mcm2, 4 6, which both destabilizes Mcm2–5 interface inhibits ATPase activity. Using X-ray crystallography, contains two winged-helix domains in C-terminal half protein a catalytically inactive dioxygenase-related N-terminal domain, is important for loading, but not subsequent replication. We...
Abstract Intraflagellar transport (IFT) trains, the polymers composed of two multi-subunit complexes, IFT-A and IFT-B, carry out bidirectional intracellular in cilia, vital for cilia biogenesis signaling. plays crucial roles ciliary import membrane proteins retrograde cargo trafficking. However, molecular architecture assembly mechanism into IFT trains vivo remains elusive. Here, we report cryo-electron microscopic structures complex from protozoa Tetrahymena thermophila . We find that...
Abstract The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global pandemic of novel disease (COVID-19). neutralizing monoclonal antibodies (mAbs) targeting the receptor-binding domain (RBD) SARS-CoV-2 are among most promising strategies to prevent and treat COVID-19. However, variants concern (VOCs) profoundly reduced efficacies mAbs vaccines approved for clinical use. Herein, we demonstrated mAb 35B5 efficiently neutralizes both wild-type (WT) VOCs, including...
The spike (S) protein of SARS-CoV-2 has been observed in three distinct pre-fusion conformations: locked, closed and open. Of these, the function locked conformation remains poorly understood. Here we engineered a S construct "S-R/x3" to arrest spikes by disulfide bond. Using this determined high-resolution structures confirming that x3 bond ability stabilize otherwise transient conformations. Structural analyses reveal wild-type can adopt two locked-1 locked-2 For D614G spike, based on...
Abstract Influenza viruses and thogotoviruses account for most recognized orthomyxoviruses. Thogotoviruses, exemplified by Thogoto virus (THOV), are capable of infecting humans using ticks as vectors. THOV transcribes mRNA without the extraneous 5′ end sequences derived from cap-snatching in influenza mRNA. Here, we report cryo-EM structures to characterize polymerase RNA synthesis initiation elongation. The demonstrate that transcription replication able start with short dinucleotide...
Chinese herbs have become a focus in cancer treatment. Icaritin, prenylflavonoid derivative from of the Epimedium genus, has selective estrogen receptor (ER) modulating activity. This study evaluates effects icaritin on apoptosis HepG2 hepatocellular carcinoma cells. Icaritin (at 5-50 µM) induced Few changes icaritin-induced were observed after pretreatment with ICI182780. Consistent induction, increased Bax/Bcl-2 ratio and caspase-3 activation Furthermore, was capable stimulating c-Jun...
To express the antimicrobial peptide cecropin D in Pichia pastoris and determine activity of expressed product, four oligonucleotide fragments were synthesized accordance with available sequences a codon bias suitable for pastoris. Sequence phosphorylated, annealed, linked cloned into expression vector pGAPZαA yeast α‑mating factor signal was used as sequence. The P. SMD1168 cells transformed by electroporation using constructed recombinant plasmid pGAPZαA‑cecropin D. We able to demonstrate...
SARS-CoV-2 Omicron variants feature highly mutated spike proteins with extraordinary abilities in evading antibodies isolated earlier the pandemic. Investigation of memory B cells from patients primarily breakthrough infections Delta variant enables isolation a number neutralizing cross-reactive to heterologous concern (VOCs) including (BA.1-BA.4). Structural studies identify altered complementarity determining region (CDR) amino acids and unusual heavy chain CDR2 insertions respectively two...
Polyphenols with a typical meta-phenol structure have been intensively investigated for scavenging of methylglyoxal (MGO) to reduce harmful substances in food. However, less attention has paid the formation level polyphenol-MGO adducts foods and vivo their absorption, metabolism, health impacts. In this study, hesperitin (HPT) was found scavenge MGO by forming two adducts, namely, 8-(1-hydroxyacetone)-hesperetin (HPT-mono-MGO) 6-(1-hydroxyacetone)-8-(1-hydroxyacetone)-hesperetin...
Abstract Continued evolution of SARS-CoV-2 generates variants to challenge antibody immunity established by infection and vaccination. A connection between population genesis virus has long been suggested but its molecular basis remains poorly understood. Here, we identify a class neutralizing public antibodies defined their shared usage VL6-57 light chains. Although heavy chains diverse genotypes are utilized, convergent HCDR3 rearrangements have observed among these cooperate with germline...
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of a potentially fatal disease named coronavirus 2019 (COVID-19), has raised significant public health concerns globally. To date, COVID-19 pandemic caused millions people to be infected with SARS-CoV-2 worldwide. It been known since 2003 SARS epidemic that coronaviruses (CoVs) have large RNA genomes, replication which requires an RNA-dependent replication/transcription complex. CoV nonstructural proteins...
SARS-CoV-2 spike protein (S) is structurally dynamic and has been observed by cryo-EM to adopt a variety of prefusion conformations that can be categorized as locked, closed, open. S-trimers adopting locked are tightly packed featuring structural elements incompatible with RBD in the “up” position. For S, it shown transient under neutral pH. Probably because their transience, remain largely uncharacterized for SARS-CoV-1 S. In this study, we introduced x1, x2, x3 disulfides into Some these...
Current noninvasive prenatal testing (NIPT) based on cell-free DNA (cfDNA) mainly targets the detection of chromosome aberrations but not dominant single-gene disorders (dSGDs). This prospective pilot study aims to evaluate clinical utility a plasma cfDNA and targeted next-generation sequencing-based NIPT approach for dSGDs (NIPT-dSGD), with particular focus neurodevelopmental (NDDs). The NIPT-dSGD method 34 genes, including 25 correlated NDDs nine Noonan spectrum, skeletal,...
High-affinity and specific binding toward the human angiotensin-converting enzyme 2 (hACE2) receptor by severe acute respiratory syndrome coronavirus (SARS)–related coronaviruses (SARSr-CoVs) remains incompletely understood. We report cryo–electron microscopy structures of eight different S-proteins from SARSr-CoVs found across Asia, Europe, Africa. These all adopt tightly packed, locked, prefusion conformations. enable classification SARSr-CoV into three types, based on their...
The pandemic outbreak of coronavirus disease 2019 (COVID-19) across the world has led to millions infection cases and caused a global public health crisis. Current research suggests that SARS-CoV-2 is highly contagious spreads rapidly through communities. To understand mechanisms viral replication, it imperative investigate replicase, huge protein complex comprising up 16 nonstructural associated host proteins, which most promising antiviral target for inhibiting genome replication...