A5084906957- RNA Research and Splicing
- RNA modifications and cancer
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Cancer-related gene regulation
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- RNA and protein synthesis mechanisms
- Biomedical Ethics and Regulation
- RNA Interference and Gene Delivery
- Renal and related cancers
- Histone Deacetylase Inhibitors Research
- Lipoproteins and Cardiovascular Health
- Viral Infections and Outbreaks Research
- Tissue Engineering and Regenerative Medicine
- Neurogenesis and neuroplasticity mechanisms
- Cellular transport and secretion
- Cholesterol and Lipid Metabolism
- 3D Printing in Biomedical Research
- Microtubule and mitosis dynamics
- Protein Degradation and Inhibitors
- Neuroblastoma Research and Treatments
Guangzhou Institutes of Biomedicine and Health
2015-2024
Chinese Academy of Sciences
2014-2024
University of Hong Kong
2022-2024
Northwestern University
2021-2024
State Key Laboratory of Respiratory Disease
2011-2024
Guangzhou Regenerative Medicine and Health Guangdong Laboratory
2011-2022
University of Chinese Academy of Sciences
2020-2022
Guangzhou Medical University
2011-2020
University of Science and Technology of China
2011
Chinese Academy of Medical Sciences & Peking Union Medical College
2011
MicroRNAs (miRNAs) are emerging critical regulators of cell function that frequently reside in clusters throughout the genome. They influence a myriad functions, including generation induced pluripotent stem cells, also termed reprogramming. Here, we have successfully delivered entire miRNA into reprogramming fibroblasts using retroviral vectors. This strategy avoids caveats associated with transient transfection chemically synthesized mimics. Overexpression 2 clusters, 106a-363 and...
Little is known how lincRNAs are involved in skeletal myogenesis. Here we describe the discovery of Linc-YY1 from promoter transcription factor (TF) Yin Yang 1 (YY1) gene. We demonstrate that dynamically regulated during myogenesis vitro and vivo. Gain or loss function C2C12 myoblasts muscle satellite cells alters myogenic differentiation injured muscles has an impact on course regeneration. interacts with YY1 through its middle domain, to evict YY1/Polycomb repressive complex (PRC2) target...
microRNAs play an important roles in cell growth, differentiation, proliferation and apoptosis. They can function either as tumor suppressors or oncogenes. We found that the overexpression of miR-192 inhibited A549, H460 95D cells, tumorigenesis a nude mouse model. Both caspase-7 PARP protein were activated by miR-192, thus suggesting induces apoptosis through caspase pathway. Further studies showed retinoblastoma 1 (RB1) is direct target miR-192. Over-expression decreased RB1 mRNA levels...
Abstract Malat1 is one of the most abundant long non-coding RNAs in various cell types; its exact cellular function still a matter intense investigation. In this study we characterized skeletal muscle cells and regeneration. Utilizing both vitro vivo assays, demonstrate that has role regulating gene expression during myogenic differentiation myoblast cells. Specifically, found knockdown accelerates cultured Consistently, knockout mice display enhanced regeneration after injury deletion...
Emerging evidence supports roles of enhancer RNAs (eRNAs) in regulating target gene. Here, we study eRNA regulation and function during skeletal myoblast differentiation. We provide a panoramic view transcription categorization eRNAs. Master factor MyoD is crucial activating production. Super (se) generated seRNA-1 -2 promote myogenic differentiation vitro vivo. regulates expression levels two nearby genes, myoglobin (Mb) apolipoprotein L6 (Apol6), by binding to heterogeneous nuclear...
N6-methyladenosine (m6A), the most abundant reversible modification on eukaryote messenger RNA, is recognized by a series of readers, including YT521-B homology domain family (YTHDF) proteins, which are coupled to perform physiological functions. Here, we report that YTHDF2 and YTHDF3, but not YTHDF1, required for reprogramming somatic cells into induced pluripotent stem (iPSCs). Mechanistically, found YTHDF3 recruits PAN2-PAN3 deadenylase complex conduces promoting mRNA clearance genes,...
Abstract Some transcription factors that specifically bind double-stranded DNA appear to also function as RNA-binding proteins. Here, we demonstrate the factor Sox2 is able directly RNA in vitro well mouse and human cells. targets via a 60-amino-acid binding motif (RBM) positioned C-terminally of high mobility group (HMG) box. can associate with simultaneously form ternary RNA/Sox2/DNA complexes. Deletion RBM does not affect selection target genes but mitigates pluripotency related...
Abstract Influenza viruses and thogotoviruses account for most recognized orthomyxoviruses. Thogotoviruses, exemplified by Thogoto virus (THOV), are capable of infecting humans using ticks as vectors. THOV transcribes mRNA without the extraneous 5′ end sequences derived from cap-snatching in influenza mRNA. Here, we report cryo-EM structures to characterize polymerase RNA synthesis initiation elongation. The demonstrate that transcription replication able start with short dinucleotide...
The interplay between the Yamanaka factors (OCT4, SOX2, KLF4 and c-MYC) transcriptional/epigenetic co-regulators in somatic cell reprogramming is incompletely understood. Here, we demonstrate that histone H3 lysine 27 trimethylation (H3K27me3) demethylase JMJD3 plays conflicting roles mouse reprogramming. On one side, induces pro-senescence factor Ink4a degrades pluripotency regulator PHF20 a factor-independent manner. other specifically recruited by to reduce H3K27me3 at both enhancers...