A5075043640- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Cancer-related gene regulation
- Infant Nutrition and Health
- Reproductive System and Pregnancy
- Neonatal Respiratory Health Research
- Ferroptosis and cancer prognosis
- Genetic Syndromes and Imprinting
- Endometriosis Research and Treatment
- Pluripotent Stem Cells Research
- Viral gastroenteritis research and epidemiology
- Food composition and properties
- Reproductive Biology and Fertility
- Gut microbiota and health
- Proteins in Food Systems
- Cancer-related molecular mechanisms research
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Polysaccharides Composition and Applications
- MicroRNA in disease regulation
- Parasitic Diseases Research and Treatment
- Diet and metabolism studies
- Parasites and Host Interactions
- Microbial Metabolic Engineering and Bioproduction
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
Chinese Academy of Agricultural Sciences
2016-2025
University of Copenhagen
2019-2025
Agricultural Genomics Institute at Shenzhen
2016-2025
China Agricultural University
2013-2025
Chinese Academy of Sciences
2024-2025
Ministry of Agriculture and Rural Affairs
2024-2025
Shandong University
2011-2025
Jining Medical University
2025
Institute of Zoology
2025
Sanya University
2025
Abstract Integrating epigenetic data with genome-wide association study (GWAS) results can reveal disease mechanisms. The genome sequence itself also shapes the epigenome, CpG density and transcription factor binding sites (TFBSs) strongly encoding DNA methylome. Therefore, genetic polymorphism impacts on observed epigenome. Furthermore, large variants alter signal dosage. Here, we identify methylation variability between GWAS-SNP risk non-risk haplotypes. In three subsets comprising 3128...
Abstract Chronic pain is a global public health problem, but the underlying molecular mechanisms are not fully understood. Here we examine genome-wide DNA methylation, first in 50 identical twins discordant for heat sensitivity and then further unrelated individuals. Whole-blood methylation was characterized at 5.2 million loci by MeDIP sequencing assessed longitudinally to identify differentially methylated regions associated with high or low (pain DMRs). Nine meta-analysis DMRs show robust...
T and B cell receptors (TCRs BCRs) play a pivotal role in the adaptive immune system by recognizing an enormous variety of external internal antigens. Understanding these is critical for exploring process immunoreaction exploiting potential applications immunotherapy antibody drug design. Although large number samples have had their TCR BCR repertoires sequenced using high-throughput sequencing recent years, very few databases been constructed to store kinds data. To resolve this issue, we...
Abstract DNA methylation has a great potential for understanding the aetiology of common complex traits such as Type 2 diabetes (T2D). Here we perform genome-wide methylated immunoprecipitation sequencing (MeDIP-seq) in whole-blood-derived from 27 monozygotic twin pairs and follow up results with replication integrated omics analyses. We identify predominately hypermethylated T2D-related differentially regions (DMRs) replicate top signals 42 unrelated T2D cases 221 controls. The strongest...
Although genetic variation is believed to contribute an individual's susceptibility major depressive disorder, genome-wide association studies have not yet identified associations that could explain the full etiology of disease. Epigenetics increasingly play a role in development common clinical phenotypes, including disorder. Genome-wide MeDIP-Sequencing was carried out on total 50 monozygotic twin pairs from UK and Australia are discordant for depression. We show disorder associated with...
This research sought to examine how the physicochemical characteristics of soy globulins and different processing techniques influence gel properties yogurt. The goal was improve these rectify any texture issues in yogurt, ultimately aiming produce premium-quality plant-based In this study, investigation focused on examining impact 7S/11S, homogenization pressure, glycation modified with glucose A yogurt superior successfully developed using a 7S/11S globulin-glucose conjugate at 1:3 ratio...
Abstract Dietary fiber influences the composition and metabolic activity of microbial communities, impacting disease development. Current understanding intricate fiber‐microbe‐disease tripartite relationship remains fragmented elusive, urging a systematic investigation. Here, we focused on microbiota disturbance as robust index to mitigate various confounding factors developed Bio‐taxonomic Hierarchy Weighted Aggregation (BHWA) algorithm integrate multi‐taxonomy data, thereby illuminating...
Abstract Background DNA methylation plays an essential role in regulating gene expression under a variety of conditions and it has therefore been hypothesized to underlie the transitions between life cycle stages parasitic nematodes. So far, however, 5'-cytosine not detected during any developmental stage nematode Caenorhabditis elegans . Given new availability high-resolution detection methods, investigation can now be carried out. Results Here, using MethylC-seq, we present first study...
Reduced representation bisulfite sequencing (RRBS) was developed to measure DNA methylation of high-CG regions at single base-pair resolution, and has been widely used because its minimal requirements cost efficacy; however, the CpG coverage genomic is restricted important with low-CG will be ignored in profiling. This method could improved generate a more comprehensive representation.Based on silico simulation enzyme digestion human mouse genomes, we have optimized current single-enzyme...
Circ-Foxo3 is a circRNA encoded by the human FOXO3 gene and works as sponge for potential microRNAs (miRNAs) to regulate cancer progression. However, role of circ-Foxo3 in esophageal squamous cell (ESCC) not clear. In this study, was lowly expressed lines ESCC tissues. Meanwhile, overexpression inhibited growth, migration, invasion, whether vivo or vitro. Mechanically, we found miRNA target, miR-23a, which negatively correlated with ESCC. Then, luciferase assay confirmed relationship between...
The serious feeding- and microbiota-associated intestinal disease, necrotizing enterocolitis (NEC), occurs mainly in infants born prematurely (5-10% of all newborns) most frequently after formula-feeding. We hypothesized that changes gene methylation is involved the prenatal maturation intestine its response to first days formula feeding, potentially leading NEC preterm pigs used as models for infants. Reduced Representation Bisulfite Sequencing (RRBS) was assess if DNA are associated with...
Abstract Background Differences in 5-hydroxymethylcytosine, 5hmC, distributions may complicate previous observations of abnormal cytosine methylation statuses that are used for the identification new tumor suppressor gene candidates relevant to human hepatocarcinogenesis. The simultaneous detection 5-methylcytosine and 5-hydroxymethylcytosine is likely stimulate discovery aberrantly methylated genes with increased accuracy hepatocellular carcinoma. Results Here, we performed...
Epigenetic alterations, such as aberrant DNA methylation of promoter and enhancer regions, which lead to atypical gene expression, have been associated with carcinogenesis. In hepatocellular carcinoma (HCC), genome-wide analysis has only recently used. For a better understanding hepatocarcinogenesis, we applied an even higher resolution the methylome identify previously unknown regions genes differentially methylated in HCC. Optimized liquid hybridization capture-based bisulfite sequencing...
Critical epigenetic regulation of primate embryogenesis entails DNA methylome changes. Here we report genome-wide composition, patterning, and stage-specific dynamics methylation in pre-implantation rhesus monkey embryos as well male female gametes studied using an optimized tagmentation-based whole-genome bisulfite sequencing method. We show that upon fertilization, both paternal maternal genomes undergo active demethylation, de novo is also initiated the same period. By 8-cell stage,...
The association of in vitro fertilisation (IVF) and DNA methylation has been studied predominantly at regulatory regions imprinted genes just thousands the ~28 million CpG sites human genome. We investigated links between IVF patterns whole cord blood cells (n = 98) mononuclear 82) from newborn twins using genome-wide methylated immunoprecipitation coupled with deep sequencing. At a false discovery rate (FDR) 5%, we identified one significant change linked to conception via IVF, which was...
N6-methyladenosine (m6A) represents the most abundantly occurring mRNA modification and is involved in regulation of skeletal muscle development. However, status function m6A methylation prenatal myogenesis remains unclear. In this study, we first demonstrated that knockdown METTL14, an methyltransferase, inhibited differentiation promoted proliferation C2C12 myoblast cells. Then, using a refined m6A-specific methylated RNA immunoprecipitation (RIP) with next generation sequencing...
Abstract Cancer chemoresistance is regulated by complex genetic and epigenetic networks. In this study, the features of gene expression, methylation, microRNA (miRNA) expression were investigated with high-throughput sequencing in human breast cancer MCF-7 cells resistant to adriamycin (MCF-7/ADM) paclitaxel (MCF-7/PTX). We found that: ① both chemoresistant cell lines had similar, massive changes miRNA versus chemosensitive controls. ② Pairwise integration data highlighted sets genes that...
Abstract Context: Skeletal muscle mass (SMM) is one of the major components human body composition, with deviations from normal values often leading to sarcopenia. Objective: Our aim was conduct a genome-wide DNA methylation study in an attempt identify potential genomic regions associated SMM. Design: This mixed cross-sectional and longitudinal study. Setting: Community-based Participants: A total 1550 middle-aged United Kingdom twins (monozygotic [MZ] dizygotic [DZ]), 297 which were...