A5045355349- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Gut microbiota and health
- Infant Nutrition and Health
- Cancer-related gene regulation
- Liver Disease Diagnosis and Treatment
- Pediatric health and respiratory diseases
- Cancer-related molecular mechanisms research
- Breastfeeding Practices and Influences
- Ferroptosis and cancer prognosis
- Oral health in cancer treatment
- Reproductive System and Pregnancy
- Infant Health and Development
- Neurological Disease Mechanisms and Treatments
- Gastrointestinal motility and disorders
- Genomics and Phylogenetic Studies
- Bladder and Urothelial Cancer Treatments
- Immune cells in cancer
- Antibiotics Pharmacokinetics and Efficacy
- Neutropenia and Cancer Infections
- Antibiotic Resistance in Bacteria
- Genomic variations and chromosomal abnormalities
- Metabolism, Diabetes, and Cancer
- Inflammatory Bowel Disease
- Genetic factors in colorectal cancer
First Affiliated Hospital of Jinan University
2015-2025
Chinese Academy of Sciences
2024-2025
BGI Group (China)
2013-2025
BGI Genomics
2024-2025
Zhejiang Cancer Hospital
2023-2025
Shanxi University
2023-2025
Ningxia Medical University
2024
University of Macau
2023-2024
Central South University
2023-2024
Xiangya Hospital Central South University
2023-2024
Huangqin decoction (HQD), a classical traditional Chinese medicinal formula, has been commonly used to treat gastrointestinal diseases for thousands of years. We investigated the anti-inflammatory effects and underlying mechanisms HQD on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC).Experimental mice were given 3% DSS, (2.275, 4.55, 9.1 g/kg), or mesalazine (ME, 200 mg/kg) orally 7 days. Body weight loss, disease activity index (DAI), colon length, histology, levels...
Reduced representation bisulfite sequencing (RRBS) was developed to measure DNA methylation of high-CG regions at single base-pair resolution, and has been widely used because its minimal requirements cost efficacy; however, the CpG coverage genomic is restricted important with low-CG will be ignored in profiling. This method could improved generate a more comprehensive representation.Based on silico simulation enzyme digestion human mouse genomes, we have optimized current single-enzyme...
Intestinal mucositis causes great suffering to cancer patients who undergo chemotherapy and radiotherapy. Owing the uncertain side effects of anticancer drugs attenuate patients' intestinal mucositis, many studies focused on traditional Chinese medicine (TCM). Patchouli alcohol (PA) is an active compound extracted from Pogostemon cablin, has potent gastrointestinal protective effect. However, whether PA effect still unknown. Therefore, we established a rat model via intraperitoneal injection...
Predictive drug testing of patient-derived tumor organoids (PDTOs) holds promise for personalizing treatment metastatic colorectal cancer (mCRC), but prospective data are limited to chemotherapy regimens with conflicting results. We describe a unified framework PDTO-based predictive across standard-of-care and biologic targeted therapy options. In an Australian community cohort, PDTO predictions based on treatment-naive patients (n = 56) response rates from first-line mCRC clinical trials...
ABSTRACT For embryo implantation and fetal development, the maternal immune system undergoes dramatic changes. The mechanisms involved in inducing alterations of immunity have not been fully clarified. Gut microbiome metabolites were thought to influence host response. During normal pregnancy, notable changes occur gut microbiota metabolites. However, relationship these function during pregnancy remains unclear. In this study, we examined microbiota, fecal metabolites, plasma cytokines...
This study aimed to explore the potential mechanisms of Buyang Huanwu Decoction (BHD) in regulating AKT/TP53 pathway and reducing inflammatory responses for treatment chronic cerebral ischemia (CCI) using UHPLC-QE-MS combined with network pharmacology, molecular docking techniques, animal experiment validation.
Acupuncture significantly improves cognitive dysfunction in rats with chronic cerebral ischemia. However, the underlying signaling pathways remain unclear. This study investigates role of CKLF1/HIF-1α/VEGF/Notch1 pathway acupuncture-mediated improvement function Male SD were randomly divided into normal control group, sham-operated 2-VO model + acupuncture and Ginaton group (Ginkgo biloba extract 14.4 mg/kg/day), 10 each group. The received at Shuigou, Baihui, bilateral Fengchi, Zusanli...
Abstract Background Differences in 5-hydroxymethylcytosine, 5hmC, distributions may complicate previous observations of abnormal cytosine methylation statuses that are used for the identification new tumor suppressor gene candidates relevant to human hepatocarcinogenesis. The simultaneous detection 5-methylcytosine and 5-hydroxymethylcytosine is likely stimulate discovery aberrantly methylated genes with increased accuracy hepatocellular carcinoma. Results Here, we performed...
Abstract Cell embedment into a solid support matrix is considered essential for the culture of intestinal epithelial organoids and tumoroids, but this technique presents challenges that impede scalable expansion, experimental manipulation, high-throughput screening diagnostic applications. We have developed low-viscosity (LVM) suspension method enables efficient establishment propagation tumoroids from human large intestine. Organoids cultured in LVM recapitulate morphological development...
Hepatic endothelial function is central to the development of nonalcoholic steatohepatitis (NASH). Curcumin (Cur) reportedly hepatoprotective, however, it remains unknown whether Cur improves hepatic in NASH. Additionally, poor bioavailability renders difficult elucidate its hepatoprotective effect, hence, biotransformation should be considered. Herein, we investigated effects and mechanisms bioconversion on against high-fat diet-induced NASH rats. The results revealed that improved lipid...
The consumption of probiotics and fermented foods has been very popular in recent decades. primary aim our study was to evaluate the effect on gut microbiota changes inflammatory cytokines after an average 6.7 weeks probiotic administration among normal pregnant women. Thirty-two healthy women at 32 gestation were recruited divided into two groups. group ingested combined until birth. base characteristics control groups showed no significant differences. structure fecal genus level varied...
In recent years, it has become evident that early-life intestinal flora plays a pivotal role in determining human health. Consequently, is imperative to explore the establishment of neonatal and its influencing factors. Early influenced by multitude factors, including maternal infant-related as well external environment. This review summarizes colonization mechanism early life newborns discussed their influence on flora, taking into account factors such delivery mode, gestational age feeding...
Whole-genome bisulfite sequencing (WGBS) technology can provide comprehensive DNA methylation at a single-base resolution on genome-wide scale, and is considered to be the gold standard for detection of 5-methylcytosine (5 mC). However, International Human Epigenome Consortium propose full methylome should have least 30 fold redundant coverage reference genome from single biological replicate. Therefore, it remains cost prohibitive large-scale studies. To find solution, DNBSEQ-Tx was...
DNBSEQ platforms have been widely used for variation detection, including single-nucleotide variants (SNVs) and short insertions deletions (INDELs), which is comparable to Illumina. However, the performance even characteristics of structural variations (SVs) detection using are still unclear. In this study, we assessed SVs 40 tools on eight whole-genome sequencing (WGS) datasets two Illumina WGS NA12878. Our findings confirmed that same tool was highly consistent, with correlations greater...
Background DNA methylation aberration and microRNA (miRNA) deregulation have been observed in many types of cancers. A systematic study methylome transcriptome bladder urothelial carcinoma has never reported. Methodology/Principal Findings The was profiled by modified methylation-specific digital karyotyping (MMSDK) the expression mRNAs miRNAs analyzed gene (DGE) sequencing tumors matched normal adjacent tissues obtained from 9 patients. We found that a set significantly enriched pathways...
5-methylcytosine (5-mC) can be oxidized to 5-hydroxymethylcytosine (5-hmC). Genome-wide profiling of 5-hmC thus far indicates may not only an intermediate form DNA demethylation but could also constitute epigenetic mark per se. Here we describe a cost-effective and selective method detect both the hydroxymethylation methylation status cytosines in subset human genome. This involves glucosylation residues, short-Sequence tag generation high-throughput sequencing. We tested this by screening...