A5101582966- Advanced Electron Microscopy Techniques and Applications
- Advanced Antenna and Metasurface Technologies
- Antenna Design and Analysis
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Electron and X-Ray Spectroscopy Techniques
- Nuclear Structure and Function
- Cyclopropane Reaction Mechanisms
- RNA Research and Splicing
- Metamaterials and Metasurfaces Applications
- Epigenetics and DNA Methylation
- SARS-CoV-2 and COVID-19 Research
- Catalytic C–H Functionalization Methods
- Cancer-related gene regulation
- Advanced X-ray Imaging Techniques
- RNA modifications and cancer
- Fluorine in Organic Chemistry
- Animal Virus Infections Studies
- Micro and Nano Robotics
- Viral gastroenteritis research and epidemiology
- Catalytic Cross-Coupling Reactions
- Inorganic Fluorides and Related Compounds
- Renal and related cancers
- Microtubule and mitosis dynamics
- Protist diversity and phylogeny
Xi'an University of Science and Technology
2022-2024
Chinese Academy of Sciences
2014-2023
Institute of Biophysics
2021-2023
Zhejiang University
2001-2022
Nanjing Agricultural University
2022
University of Chinese Academy of Sciences
2019-2021
Jinan University
2015-2020
Inner Mongolia Normal University
2018
Lanzhou University
2018
Shanghai Institute of Organic Chemistry
2014-2015
The aim of the study was to establish life cycle severe acute respiratory syndrome-associated coronavirus (SARS CoV) in host cells and determine pathogenesis SARS. Vero E6 (African green monkey kidney cells) were inoculated with SARS for 3, 7, 24, 48, 72 hr, respectively, observed under electron microscope. It found that entered through membrane fusion instead endocytosis, then nucleocapsids assembled RER matured by budding into smooth vesicles, which derived from Golgi apparatus. vesicles...
The propulsion for mammalian sperm swimming is generated by flagella beating. Microtubule doublets (DMTs) along with microtubule inner proteins (MIPs) are essential structural blocks of flagella. However, the intricate molecular architecture intact DMT remains elusive. Here, in situ cryo-electron tomography, we solved in-cell structure mouse at 4.5-7.5 Å resolutions, and built its model 36 kinds MIPs 48 nm periodicity. We identified multiple copies Tektin5 that reinforce Tektin bundle,...
A new strategy for the construction of a CC–CF3 subunit has been developed via CuBr-catalyzed domino cyclization–trifluoromethylation homopropargyl amines with Umemoto's reagent. 4-Trifluoromethyl-2,3-dihydro-pyrroliums were produced in high yields. The usefulness these products demonstrated by transformation them into various other trifluoromethylated molecules.
Abstract The nuclear pore complex (NPC), one of the largest protein complexes in eukaryotes, serves as a physical gate to regulate nucleocytoplasmic transport. Here, we determined 8 Å resolution cryo-electron microscopic (cryo-EM) structure outer rings containing ring (NR) and cytoplasmic (CR) from Xenopus laevis NPC, with local resolutions reaching 4.9 Å. With aid AlphaFold2, managed build pseudoatomic model rings, including Y flanking components. In this most comprehensive accurate date,...
Abstract Coronaviruses spike (S) glycoproteins mediate viral entry into host cells by binding to receptors. However, how the S1 subunit undergoes conformational changes for receptor recognition has not been elucidated in Alphacoronavirus . Here, we report cryo-EM structures of HCoV-229E S trimer prefusion state with two conformations. The activated conformation may pose potential exposure S1-RBDs decreasing interaction area between and surrounding S1-NTDs compared closed conformation....
5-methylcytosine (5-mC) can be oxidized to 5-hydroxymethylcytosine (5-hmC). Genome-wide profiling of 5-hmC thus far indicates may not only an intermediate form DNA demethylation but could also constitute epigenetic mark per se. Here we describe a cost-effective and selective method detect both the hydroxymethylation methylation status cytosines in subset human genome. This involves glucosylation residues, short-Sequence tag generation high-throughput sequencing. We tested this by screening...
ABSTRACT In this article, we numerically and experimentally investigate the electromagnetic responses of multiple‐band reflective polarization based on complementary L‐shaped metamaterial. The proposed converter can convert a linearly polarized wave to its cross‐polarized at five resonant frequencies, which is also circularly other six frequencies. Furthermore, incident be transformed into same rotational direction frequencies after reflection, different from reflection by conventional...
This account summarizes our recent work on palladacycles in organic synthesis and describes some unique catalytic properties of as transition-metal catalysts for useful transformations. Asymmetric induction has been realized by using chiral catalysts. The influence the C−Pd bond their activity revealed. reaction selectivity can be switched appropriate choice an sp<sup>2</sup>- or sp<sup>3</sup>-hybridized <i>C</i>,<i>P</i>-palladacycle catalyst. 1 Introduction 2 Discovery Use Palladacycles...
Identification of singleton P2X7 inhibitor 1 from HTS gave a pharmacophore that eventually turned into potential clinical candidates 17 and 19. During development, number issues were successfully addressed, such as metabolic stability, plasma GSH adduct formation, aniline mutagenicity. Thus, careful modification the molecule, conversion 1,4-dihydropyridinone to 1,2-dihydropyridinone system, proper substitution at C-5″, in some cases addition fluorine atoms ring allowed for identification...
A practical asymmetric synthesis of a 3,7-diazabicyclo[3.3.0]octane derivative (1), representative new class potent CCR5 receptor antagonists, is described. The benzylamine stereogenic center 1 was introduced by ruthenium-catalyzed reductive amination using (R)-MeOBIPHEP as ligand. Aldehyde 4, prepared Parikh−Doering oxidation, used without workup in the reaction, which not only simplified process but also overcame instability 4. core obtained [3 + 2] cycloaddition.
The 17-norpimarane diterpene is a small group of natural products. A new member, icacinlactone H (1), and rearranged with an unprecedented carbon skeleton, icacintrichantholide (2), were isolated from Icacina trichantha. Their structures including the absolute configuration elucidated by spectroscopic analysis, single-crystal X-ray diffraction, electronic circular dichroism analysis. plausible biogenetic pathway for 1 2 proposed. Both showed no significant activity against herpes simplex...
As a well-known epigenomic modification, DNA methylation is found to be common in plants and plays an important role many biological processes. Relying on the unique feature of methylation-dependent digestion, family methylation-requiring restriction-like endonuclease, such as MspJI its homologs, was suggested for potential usage detection.In this study, we combine digestion electrophoretic band selection with next generation high-throughput sequencing technology detect 5-methylcytosines...
Abstract A new efficient strategy for the synthesis of 3‐trifluoromethyl‐indoles was developed through tandem cyclization/trifluoromethylation 2‐alkynylanilines and Umemoto's reagent under effect CuBr. The reaction features use cheap copper salt, mild conditions high yield products. preliminary mechanism study indicates solvent dimethylacetamide acts as role dealkylation.
Six new pimarane derivatives, including two di-nor-pimaranes (1, 2), 17-nor-pimaranes (3, 4), and 17-nor-(9β-H)-pimaranes (5, 6), were isolated from the tuber of Icacina trichantha. Their structures elucidated based on spectroscopic HRMS data. The absolute configurations 1 5 determined by single-crystal X-ray diffraction, that 2 was established electronic circular dichroism data analysis. Compound 3 possesses a unique C-20 acetal moiety. This is first report isolation di-nor-(9β-H)-pimarane...
Porcine epidemic diarrhea virus (PEDV) can infect pigs of all ages, especially piglets. PEDV has spread across Asia since the 1980s. The highly virulent variant broke out on a large scale and caused huge economic losses to pig industry in late 2010 China. Rapid detection methods with high specificity sensitivity are urgently needed for diagnosis control disease. In this study, we divided S1 gene into three segments constructed recombinant plasmids pFastBac1-S1T1 (aa 21-279), pFastBac1-S1T2...
Cryo-electron microscopy (cryo-EM), especially single-particle analysis, has become a powerful technique for visualizing the structure of biological macromolecules at high resolution. However, data acquisition in cryo-EM is time consuming because it requires collection thousands images to achieve high-quality reconstruction. Real-time preprocessing and display can greatly enhance efficiency quality collection. This study presents AutoEMage , new open-source software package that automates...