A5091129802- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Single-cell and spatial transcriptomics
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Nitrogen and Sulfur Effects on Brassica
- Epigenetics and DNA Methylation
- RNA regulation and disease
- Genetics and Neurodevelopmental Disorders
- Selenium in Biological Systems
- Photosynthetic Processes and Mechanisms
- Renal and related cancers
- Prenatal Screening and Diagnostics
- RNA Interference and Gene Delivery
- Reproductive Biology and Fertility
- Lipid metabolism and biosynthesis
- Animal Genetics and Reproduction
- Virus-based gene therapy research
- Congenital heart defects research
- Tissue Engineering and Regenerative Medicine
- Nuclear Structure and Function
- Genetic Syndromes and Imprinting
- RNA Research and Splicing
- Assisted Reproductive Technology and Twin Pregnancy
Kunming University of Science and Technology
2015-2025
Yunnan Yingmao Biotechnology (China)
2014-2025
Huainan Normal University
2025
Hunan Agricultural University
2020-2024
Enshi Tujia and Miao Autonomous Prefecture Academy of Agricultural Sciences
2020-2024
Shanxi University
2021-2024
Anna Needs Neuroblastoma Answers
2021
Yunnan Provincial Science and Technology Department
2017-2018
Ajou University Hospital
2016
Kunming Medical University
2014
CRISPR/Cas9 has been used to genetically modify genomes in a variety of species, including non-human primates. Unfortunately, this new technology does cause mosaic mutations, and we do not yet know whether such mutations can functionally disrupt the targeted gene or pathology seen human disease. Addressing these issues is necessary if are generate large animal models diseases using CRISPR/Cas9. Here target monkey dystrophin create that lead Duchenne muscular dystrophy (DMD), recessive...
The transition from peri-implantation to gastrulation in mammals entails the specification and organization of lineage progenitors into a body plan. Technical ethical challenges have limited understanding cellular molecular mechanisms that underlie this transition. We established culture system enabled development cynomolgus monkey embryos vitro for up 20 days. Cultured underwent key primate developmental stages, including segregation, bilaminar disc formation, amniotic yolk sac cavitation,...
Abstract Embryonic development is largely conserved among mammals. However, certain genes show divergent functions. By generating a transcriptional atlas containing >30,000 cells from post-implantation non-human primate embryos, we uncover that ISL1 , gene with well-established role in cardiogenesis, controls regulatory network amnion. CRISPR/Cas9-targeting of results embryos which do not yield viable offspring, demonstrating critically required embryogenesis. On cellular level, mutant...
Abstract Blastoids—blastocyst-like structures created in vitro—emerge as a valuable model for early embryonic development research. Non-human primates stem cell-derived blastoids are an ethically viable alternative to human counterparts, yet the low formation efficiency of monkey blastoid cavities, typically below 30%, has limited their utility. Prior research predominantly utilized cells. In this work, we demonstrate efficient generation from induced pluripotent cells and somatic cell...
Mutations in the DAX1 locus cause X-linked adrenal hypoplasia congenita (AHC) and hypogonadotropic hypogonadism (HH), which manifest with primary insufficiency incomplete or absent sexual maturation, respectively. The associated defects spermatogenesis can range from spermatogenic arrest to Sertoli cell only syndrome. Conclusions Dax1 knockout mouse models provide limited insight into AHC/HH disease mechanisms, because exhibit more extensive abnormalities testicular development, including...
Abstract Many human genetic diseases, including Hutchinson-Gilford progeria syndrome (HGPS), are caused by single point mutations. HGPS is a rare disorder that causes premature aging and usually de novo mutation in the LMNA gene. Base editors (BEs) composed of cytidine deaminase fused to CRISPR/Cas9 nickase highly efficient at inducing C T base conversions programmable manner can be used generate animal disease models with amino-acid substitutions. Here, we generated first monkey model...
Critical epigenetic regulation of primate embryogenesis entails DNA methylome changes. Here we report genome-wide composition, patterning, and stage-specific dynamics methylation in pre-implantation rhesus monkey embryos as well male female gametes studied using an optimized tagmentation-based whole-genome bisulfite sequencing method. We show that upon fertilization, both paternal maternal genomes undergo active demethylation, de novo is also initiated the same period. By 8-cell stage,...
To solve the clinical transformation dilemma of lamin A/C (LMNA)-mutated dilated cardiomyopathy (LMD), we developed an LMNA-mutated primate model based on similarity between phenotype primates and humans. We screened out patients with LMD compared data TTN-mutated mutation-free to obtain unique phenotype. After establishment LMNA c.357-2A>G model, were continuously observed for 48 months, echocardiographic, electrophysiological, histologic, transcriptional recorded. The was found highly...
Annexins (ANN) are a multigene, evolutionarily conserved family of calcium-dependent and phospholipid-binding proteins that play important roles in plant development stress resistance. However, systematic comprehensive analysis ANN genes Brassicaceae species (Brassica rapa, Brassica oleracea, napus) has not yet been reported. In this study, we identified 13, 12, 26 B. napus, respectively. About half these were clustered on various chromosomes. Molecular evolutionary showed the highly...
Abstract Background TIFY is a plant-specific protein family with diversity of functions in plant development and responses to stress hormones, which contains JASMONATE ZIM-domain (JAZ), TIFY, PPD ZML subfamilies. Despite extensive studies many other species, has not yet been characterized Brassica napus . Results In this study, we identified 77, 36 39 genes the genome B. , rapa oleracea respectively. phylogenetic analysis indicated 170 proteins from Arabidopsis could be divided into 11...
Mutations of <i>PTEN-induced kinase I</i> (<i>PINK1</i>) cause early-onset Parkinson's disease (PD) with selective neurodegeneration in humans. However, current <i>PINK1</i> knockout mouse and pig models are unable to recapitulate the typical neurodegenerative phenotypes observed PD patients. This suggests that generating non-human primates (NHPs) close humans is essential investigate unique function PINK1 primate brains. Paired single guide RNA (sgRNA)/Cas9-D10A nickases truncated...
Selenium (Se) is an essential microelement in living organisms, and has a beneficial effect on plant growth stress tolerance at low concentrations. Se biofortification commonly practiced agriculture considered the best method for increasing human uptake. This study aimed to investigate effects of fertilizer application accumulation microbial communities rhizospheric soil tomato plants. A pot experiment with five treatments, i.e. (a) non-Se as control (Ck) form (b) sodium selenite (1 mg...
Abstract Background Freezing stress is one of the major abiotic stresses that causes extensive damage to plants. LEA (Late embryogenesis abundant) proteins play a crucial role in plant growth, development, and stress. However, there limited research on function genes low-temperature Brassica napus (rapeseed). Results Total 306 potential were identified B. rapa (79) , oleracea (148) divided into eight subgroups. same subgroup had similar gene structures predicted subcellular locations. Cis...
Lysophosphatidate acyltransferase (LPAT) catalyzes the conversion of lysophosphatidic acid to phosphatidic acid, a key step in lipid biosynthesis. This study cloned four LPAT2 genes from Brassica napus : BnLPAT2-A04, A07, A09, and C08 . Functional analysis using bioinformatics, qRT-PCR (Quantitative Reverse Transcription Polymerase Chain Reaction), CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated protein 9), overexpression, transcriptome sequencing...
N6-methyladenosine (m6A) modification involves the addition of a methyl group to nitrogen atom at position six adenine in RNA. It is most prevalent type dynamic internal RNA methylation modification, plays an important role plant development and abiotic stress. The m6A facilitated by writers (m6A methyltransferases), erasers demethylation enzymes), readers methylated reading proteins). In order study characterization expression methyltransferases demethylases Brassica napus (rapeseed), we...
Monkeys are an optimal model species for developing stem cell therapies. We previously reported generating chimeric cynomolgus monkey fetuses using dome-shaped embryonic cells (dESCs). However, conventional primed pluripotent (pPSCs) lack chimera competency. Here, by altering the media in which injected morulae cultured, we observed increased survival of ESCs, induced PSCs, and somatic nuclear transfer-derived thereby enabling contributions with 0.1%-4.5% chimerism into placental tissues,...