A5041130348- Mesenchymal stem cell research
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Cancer Cells and Metastasis
- Sperm and Testicular Function
- RNA Research and Splicing
- Tissue Engineering and Regenerative Medicine
- Hematopoietic Stem Cell Transplantation
- Neurogenesis and neuroplasticity mechanisms
- Reproductive Biology and Fertility
- RNA modifications and cancer
- Transplantation: Methods and Outcomes
- Virus-based gene therapy research
- Immune Cell Function and Interaction
- MicroRNA in disease regulation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Extracellular vesicles in disease
- Single-cell and spatial transcriptomics
- Renal and related cancers
- Immune cells in cancer
- Epigenetics and DNA Methylation
- Adipose Tissue and Metabolism
- Hormonal and reproductive studies
- Neonatal Respiratory Health Research
- Organ Transplantation Techniques and Outcomes
Sun Yat-sen University
2016-2025
Key Laboratory of Guangdong Province
2007-2024
Third Affiliated Hospital of Sun Yat-sen University
2010-2022
Guangzhou Regenerative Medicine and Health Guangdong Laboratory
2020-2022
The Seventh Affiliated Hospital of Sun Yat-sen University
2021
Guangzhou Medical University
2017-2019
Zhongshan Hospital
2018
Cell Technology (China)
2017
Cell and Tissue Systems (United States)
2013
GTx (United States)
2012
Increasing evidence has supported the important role of mesenchymal stem cells (MSCs) in wound healing, however, underlying mechanism remains unclear. Recently, we have isolated a unique population MSCs from human gingiva (GMSCs) with similar cell-like properties, immunosuppressive, and anti-inflammatory functions as bone marrow-derived (BMSCs). We describe here interplay between GMSCs macrophages potential relevance skin healing. When cocultured GMSCs, acquired an M2 phenotype characterized...
Constitutive promoters are used routinely to drive ectopic gene expression. Here, we carried out a systematic comparison of eight commonly constitutive (SV40, CMV, UBC, EF1A, PGK and CAGG for mammalian systems, COPIA ACT5C Drosophila systems). We also included in the TRE promoter, which can be activated by rtTA transcriptional activator doxycycline-inducible manner. To make our findings representative, conducted variety cell types derived from several species. found that these vary...
The intermediate filament protein, nestin, is a widely employed marker of multipotent neural stem cells (NSCs). Recent in vitro studies have implicated nestin number cellular processes, but there no data yet on its vivo function. Here, we report the construction and functional characterization Nestin knockout mice. We found that these mice show embryonic lethality, with neuroepithelium developing tube exhibiting significantly fewer NSCs much higher levels apoptosis. Consistent this...
Mortality from hepatitis B virus (HBV)–related acute‐on‐chronic liver failure (ACLF) is high due to limited treatment options. Preclinical and clinical investigations have proved that with mesenchymal stromal cells (MSCs) beneficial for recovery injury. We hypothesized the outcome of HBV‐related ACLF would be improved by MSC treatment. From 2010 2013, 110 patients were enrolled in this open‐label, nonblinded randomized controlled study. The control group (n = 54) was treated standard medical...
Despite the high cure rate of T cell acute lymphoblastic leukemia (T-ALL), drug resistance to chemotherapy remains a significant clinical problem. Bone marrow mesenchymal stem cells (MSCs) protect leukemic from chemotherapy, but underlying mechanisms are poorly understood. In this study, we aimed uncover mechanism MSC-induced chemoresistance in T-ALL cells, thus providing promising therapy target.Cell viability was determined using assay kit CCK-8. The mitochondrial ROS levels were detected...
Ferroptosis is a newly discovered form of iron-dependent oxidative cell death and drives the loss neurons in spinal cord injury (SCI). Mitochondrial damage critical contributor to neuronal death, while mitochondrial quality control (MQC) an essential process for maintaining homeostasis promote survival. However, role MQC ferroptosis has not been clearly elucidated. Here, we further demonstrate that primarily suffer from SCI at single sequencing level. Mechanistically, disordered aggravates...
Skeletal muscle aging is a key contributor to age-related frailty and sarcopenia with substantial implications for global health. Here we profiled 90,902 single cells 92,259 nuclei from 17 donors map the process in adult human intercostal muscle, identifying cellular changes each compartment. We found that distinct subsets of stem exhibit decreased ribosome biogenesis genes increased CCL2 expression, causing different phenotypes. Our atlas also highlights an expansion associated...
Adaptive metabolic responses and innate metabolites hold promising therapeutic potential for stroke, while targeted interventions require a thorough understanding of underlying mechanisms. Adiposity is noted modifiable risk factor recent research suggests that it benefits neurological rehabilitation. During the early phase experimental lipidomic results showed fat depots underwent pronounced lipolysis released fatty acids (FAs) feed into consequent hepatic FA oxidation ketogenesis. Systemic...
Refractory chronic GVHD (cGVHD) is an important complication after allogeneic hematopoietic SCT and prognostic of poor outcome. MSCs are involved in tissue repair modulating immune responses vitro vivo. From April 2005 to October 2008, 19 patients with refractory cGVHD were treated derived from the BM volunteers. The median dose was 0.6 × 106 cells per kg body weight. Fourteen (73.7%) responded well MSCs, achieving a CR (n=4) or PR (n=10). immunosuppressive agent could be tapered less than...
The ability to identify and isolate lineage-specific stem cells from adult tissues could facilitate cell replacement therapy. Leydig (LCs) are the primary source of androgen in mammalian testis, prospective identification (SLCs) may offer new opportunities for treating testosterone deficiency. Here, a transgenic mouse model expressing GFP driven by Nestin (Nes) promoter, we observed Nes-GFP+ located testicular interstitial compartment where SLCs normally reside. We showed that these...
The deleterious side effects of calcineurin inhibitors have impaired long-term survival after renal allograft. New immunotherapy regimens that minimize or even eliminate are required to improve transplantation outcome. Mesenchymal stem cells (MSCs) represent a unique cell population with immunosuppressive function and prolong allograft in experimental organ transplant models.In this pilot study, donor-derived bone marrow MSCs combined sparing dose tacrolimus (50% standard dose) were...
Abstract Crohn’s disease (CD) is a chronic inflammatory bowel that difficult to treat. However, previous preclinical and clinical studies have shown mesenchymal stromal cells (MSCs) are promising therapeutic approach, whereas the exact underlying molecular mechanisms of MSCs in treating CD remain unclear. Furthermore, heterogeneity MSCs, as well vivo microenvironments may influence efficacy. In our study, we found subpopulation mouse with high expression matrix Gla protein (MGP), one members...
Abstract Abnormal cancer antioxidant capacity is considered as a potential mechanism of tumor malignancy. Modulation oxidative stress status emerging an anti-cancer treatment. Our previous studies have found that Nestin-knockdown cells were more sensitive to in non-small cell lung (NSCLC). However, the molecular by which Nestin protects from damage remains unclear. Here, we identify feedback loop between and Nrf2 maintaining redox homeostasis. Mechanistically, ESGE motif interacts with Kelch...
The bone marrow microenvironment facilitates the proliferation and survival of leukemia cells, contributing to disease relapse. Bone marrow-derived mesenchymal stem cells (MSCs) are well known promote cancer chemoresistance via soluble factors cell adhesion. However, little is about effects MSCs on mitochondrial dynamics T-cell acute lymphoblastic (T-ALL) or how this may influence these cells. Here, we tested both indirect (Transwell) direct coculture strategies, found that protected T-ALL...
mRNA 3' end processing is an essential step in gene expression. It well established that canonical eukaryotic pre-mRNA carried out within a macromolecular machinery consisting of dozens trans-acting proteins. However, it unknown whether RNAs play any role this process. Unexpectedly, we found subset small nucleolar (snoRNAs) are associated with the mammalian complex. These snoRNAs primarily interact Fip1, component cleavage and polyadenylation specificity factor (CPSF). We have functionally...
Abstract Pericytes play essential roles in blood–brain barrier (BBB) integrity and dysfunction or degeneration of pericytes is implicated a set neurological disorders although the underlying mechanism remains largely unknown. However, scarcity material sources hinders application BBB models vitro for pathophysiological studies. Additionally, whether can be used to treat elucidated. Here, we generate pericyte-like cells (PCs) from human pluripotent stem (hPSCs) through intermediate stage...
Rationale: Mesenchymal stromal cells (MSCs) are emerging as a novel therapeutic strategy for the acute ischemic stroke (AIS).However, poor targeted migration and low engraftment in lesions restrict their treatment efficacy.The brain express specific chemokine profile, while cultured MSCs lack set of corresponding receptors.Thus, we hypothesize that overexpression certain receptor might help homing improve efficacy.Methods: Using middle cerebral artery occlusion (MCAO) model stroke,...
Steroid-resistant (SR) acute graft-versus-host disease (aGVHD) lacks standard second-line treatment. Mesenchymal stromal cells (MSCs) have potential efficacy in SR aGVHD. We aimed to assess the and safety of MSCs combined with basiliximab calcineurin inhibitor as therapy for