A5022458821- interferon and immune responses
- Pluripotent Stem Cells Research
- Cancer-related molecular mechanisms research
- Endoplasmic Reticulum Stress and Disease
- RNA modifications and cancer
- Cancer Research and Treatments
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- CRISPR and Genetic Engineering
- Inflammasome and immune disorders
- Liver Disease Diagnosis and Treatment
- Immune Response and Inflammation
- Extracellular vesicles in disease
- Ubiquitin and proteasome pathways
- Phagocytosis and Immune Regulation
- NF-κB Signaling Pathways
- Renal and related cancers
- Liver physiology and pathology
- Cancer Mechanisms and Therapy
- Cancer, Hypoxia, and Metabolism
- Macrophage Migration Inhibitory Factor
- Abdominal vascular conditions and treatments
- Pancreatic function and diabetes
- Protein Degradation and Inhibitors
- Natural Compound Pharmacology Studies
Third Affiliated Hospital of Sun Yat-sen University
2015-2025
Sun Yat-sen University
2015-2025
University of Southern California
2015
Abstract Crohn’s disease (CD) is a chronic inflammatory bowel that difficult to treat. However, previous preclinical and clinical studies have shown mesenchymal stromal cells (MSCs) are promising therapeutic approach, whereas the exact underlying molecular mechanisms of MSCs in treating CD remain unclear. Furthermore, heterogeneity MSCs, as well vivo microenvironments may influence efficacy. In our study, we found subpopulation mouse with high expression matrix Gla protein (MGP), one members...
Activation of Wnt/β-catenin signaling can induce both self-renewal and differentiation in naive pluripotent embryonic stem cells (ESCs). To gain insights into the mechanism by which regulates ESC fate, we screened characterized its downstream targets. Here, show that self-renewal-promoting effect is mainly mediated two targets, Klf2 Tfcp2l1. Forced expression Tfcp2l1 not only reprogramming primed state pluripotency ESCs, but also sufficient to maintain ESCs. Conversely, downregulation...
Lipid accumulation often leads to lipotoxic injuries hepatocytes, which can cause nonalcoholic steatohepatitis. The association of inflammation with lipid in liver tissue has been studied for decades; however, key mechanisms have identified only recently. In particular, it is still unknown how hepatic regulates metabolism hepatocytes. Herein, we found that PA treatment or direct stimulation STING1 promoted, whereas deficiency impaired, MTORC1 activation, suggesting involved PA-induced...
The advent of human-induced pluripotent stem cells holds great promise for producing ample individualized hepatocytes. Although previous efforts have succeeded in generating hepatocytes from human vitro by viral-based expression transcription factors and/or addition growth during the differentiation process, safety issue viral transduction and high cost cytokines would hinder downstream applications. Recently, use small molecules has emerged as a powerful tool to induce cell fate transition...
Background Emerging evidence indicates that the cyclic GMP-AMP synthase-stimulator of interferon genes (cGAS-STING) axis plays a pivotal role in intrinsic antitumor immunity. Previous studies demonstrate conventional chemotherapy agent, teniposide, effectively promotes therapeutic efficacy programmed cell death protein-1 antibody (PD-1 Ab) through robust cGAS-STING activation. Unfortunately, cGAS expression tumor cells is reported to be severely suppressed by hypoxic status solid tumor....
Drug repurposing is currently an important approach for accelerating drug discovery and development clinical use. Hepatocellular carcinoma (HCC) presents resistance to chemotherapy, the prognosis poor due existence of liver cancer stem-like cells. In this study, we investigated effect neuroleptic agent pimozide inhibit cell maintenance tumorigenicity in HCC. Our results showed that functioned as anti-cancer HCC cells or Pimozide inhibited proliferation sphere formation capacities by inducing...
Abstract MALAT1-associated small cytoplasmic RNA (mascRNA) is a tRNA-like derived from nucleus-located long noncoding (lncRNA) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1). While MALAT1 was extensively studied and found to function in multiple cellular processes, including tumorigenesis tumor progression, the role of mascRNA largely unknown. Here we show that upregulated cancer cell lines hepatocellular carcinoma (HCC) clinical samples. Using HCC cells as model, its parent...
Numerous studies demonstrated that NLRP3 has been implicated in the pathogenesis of inflammatory bowel disease (IBD). Mesenchymal stem cells (MSCs) regulated inflammasome, which emerged as a novel therapeutic approach for treating IBD. The exact role regulating MSCs' function is unclear. Our study aimed to explore how affects effects MSCs colitis. We extracted from bone marrow C57/B6 mice and Nlrp3 KO mice, identified them using differentiation assays flow cytometry. In vitro, Both WT were...
Liver surgery during the perioperative period often leads to a significant complication known as hepatic ischemia-reperfusion (I/R) injury. Hepatic I/R injury is linked innate immune response. The cGAS-STING pathway triggers activation of through detection DNA within cells. Nevertheless, precise mechanism and significance in are yet be investigated.
Hepatic ischemia-reperfusion (I/R) injury frequently occurs during the perioperative phase of liver surgery. Inappropriate activation STING signaling can trigger excessive inflammation response to aggravate hepatic I/R injury. Dimethyl fumarate (DMF) is an FDA-approved immunomodulatory drug used treat multiple sclerosis and psoriasis due its notable anti-inflammation properties. However, mechanism targets DMF in immunomodulation remain unclear. Here, we found that suppresses cGAS-STING...
Non-alcoholic steatohepatitis (NASH), a progressive form of non-alcoholic fatty liver disease (NAFLD), is characterised by chronic inflammation, which can further progress into complications such as cirrhosis and NASH-associated hepatocellular carcinoma (HCC) therefore has become growing health problem worldwide. The type I interferon (IFN) signaling pathway plays pivotal role in inflammation; however, the molecular mechanisms underlying NAFLD/NASH from perspective innate immune response not...
Wogonin (5,7-dihydroxy-8-methoxyflavone) is an ingredient of the extracts from Scutellaria baicalensis, which has documented a wide spectrum anti-inflammatory and antitumor activities, including inhibiting regulatory T cells, regulating effector cell functions, mediating macrophage immunity. However, potential effect on B cells not been fully understood. Here, our results showed that inhibited IL-10 secretion in cells. When purified were activated by lipopolysaccharide (LPS) vitro, amount...
The selective macroautophagy/autophagy pathway is an important of protein degradation, regulating signal transduction pathways via degradation certain signaling complexes. TBK1 functions as a key in innate immunity or metabolic-associated fatty liver disease (MAFLD); however, the has not been fully investigated. Recently, we have found that HNF1A novel cargo receptor to bridge and MAP1LC3/LC3, hence promoting antiviral MAFLD.
Hepatic ischemia-reperfusion injury (IRI) is the most common cause of liver damage leading to surgical failures in hepatectomy and transplantation. Extensive inflammatory reactions oxidative responses are reported be major processes exacerbating IRI. The involvement Yes-associated protein (YAP) either process has been suggested, but role mechanism YAP IRI remain unclear. In this study, we constructed hepatocyte-specific knockout (YAP-HKO) mice induced a hepatic model. Surprisingly, amount...
Mesenchymal stem cells (MSCs) have a variety of unique properties, such as cell multipotency and immune regulation, making them attractive for use in therapy. Before infusion therapy, MSCs are required to undergo tissue separation, purification, expansion vitro certain duration. During the process MSCs, influence culture time environment can lead senescence, increased heterogeneity, function attenuation, which limits their clinical applications. We used cocktail three small-molecule...