A5051576642- Advanced biosensing and bioanalysis techniques
- Genomics and Chromatin Dynamics
- DNA Repair Mechanisms
- DNA and Nucleic Acid Chemistry
- Microtubule and mitosis dynamics
- RNA Interference and Gene Delivery
- Telomeres, Telomerase, and Senescence
- Biosensors and Analytical Detection
- RNA and protein synthesis mechanisms
- Force Microscopy Techniques and Applications
- CRISPR and Genetic Engineering
- Photosynthetic Processes and Mechanisms
- 14-3-3 protein interactions
- Bacteriophages and microbial interactions
- Mitochondrial Function and Pathology
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- RNA modifications and cancer
- Viral Infectious Diseases and Gene Expression in Insects
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Pluripotent Stem Cells Research
- Advanced Electron Microscopy Techniques and Applications
- Chromosomal and Genetic Variations
- Molecular Biology Techniques and Applications
North Carolina State University
2015-2024
Kunming University of Science and Technology
2014-2024
Shanghai First Maternity and Infant Hospital
2018-2024
Zhejiang University of Technology
2009-2024
Temple University
2017-2024
Peripheral Vascular Associates
2024
Medtronic (United States)
2024
State Key Laboratory of Pollution Control and Resource Reuse
2024
Tongji University
2023-2024
Shanghai Institute of Pollution Control and Ecological Security
2024
Amorpha-4,11-diene synthase (ADS) of Artemisia annua catalyzes the conversion farnesyl diphosphate into amorpha-4,11-diene, first committed step in biosynthesis antimalarial drug artemisinin. The promoters ADS contain two reverse-oriented TTGACC W-box cis-acting elements, which are proposed binding sites WRKY transcription factors. A full-length cDNA (AaWRKY1) was isolated from a library glandular secretory trichomes (GSTs) artemisinin is synthesized and sequestered. AaWRKY1 encodes 311...
CRISPR/Cas9 has been used to genetically modify genomes in a variety of species, including non-human primates. Unfortunately, this new technology does cause mosaic mutations, and we do not yet know whether such mutations can functionally disrupt the targeted gene or pathology seen human disease. Addressing these issues is necessary if are generate large animal models diseases using CRISPR/Cas9. Here target monkey dystrophin create that lead Duchenne muscular dystrophy (DMD), recessive...
Lowly expressed analyte in complex cytoplasmic milieu necessitates the development of non-enzymatic autocatalytic DNA circuits with high amplification and anti-interference performance. Herein, we engineered a versatile robust stimuli-responsive hybridization assembly (AHA) circuit for high-performance vivo bioanalysis. Under moderately confined condition, initiator motivated autonomous cooperative cross-activation cascade reaction catalytic generating an exponentially amplified readout...
Abstract PARP1 is a DNA-dependent ADP-Ribose transferase with ADP-ribosylation activity that triggered by DNA breaks and non-B structures to mediate their resolution. was also recently identified as component of the R-loop-associated protein-protein interaction network, suggesting potential role for in resolving this structure. R-loops are three-stranded nucleic acid consist RNA–DNA hybrid displaced non-template strand. involved crucial physiological processes but can be source genome...
DNA origami has played an important role in various biomedical applications, including biosensing, bioimaging, and drug delivery. However, the function of long scaffold involved yet to be fully exploited. Herein, we report a general strategy for construction genetically encoded by employing two complementary strands functional gene as therapy. In our design, sense antisense can directly folded into monomers their corresponding staple strands. After hybridization, assembled with precisely...
Abstract Purpose: Poly(ADP-ribose) polymerase-1 (PARP-1) is the founding member of a family enzymes that catalyze addition ADP-ribose units to proteins mediate DNA repair pathways. Ionizing radiation induces strand breaks, suggesting PARP-1 inhibition may sensitize tumor cells radiation. Experimental Design: We investigated combination with in lung cancer models. ABT-888, novel potent inhibitor, was used explore effects on irradiated tumors and vasculature. Results: ABT-888 reduced...
Increased nuclear protein O-linked beta-N-acetylglucosamine glycosylation (O-GlcNAcylation) mediated by high glucose treatment or the hyperglycemia of diabetes mellitus contributes to cardiac myocyte dysfunction. However, whether mitochondrial proteins in myocytes are also submitted O-GlcNAcylation excessive alters function is unknown. In this study, we determined if O-GlcNAcylated and explored increased linked glucose-induced dysfunction neonatal rat cardiomyocytes. By immunoprecipitation,...
One of the key functions health insurance is to provide financial protection against high costs care, yet evidence such from developing countries has been inconsistent. The current study uses case Ghana contribute pool about insurance's effects. It evaluates impact country's National Health Insurance Scheme on households' out-of-pocket spending and catastrophic expenditure.We use data a household survey conducted in two rural districts, Nkoranza Offinso, 2007, years after initiation Scheme....
The effective monitoring, identification, and quantification of pathogenic bacteria is essential for addressing serious public health issues. In this study, we present a universal facile one-step strategy sensitive selective detection using dual-molecular affinity-based Förster (fluorescence) resonance energy transfer (FRET) platform based on the recognition bacterial cell walls by antibiotic aptamer molecules, respectively. As proof concept, Vancomycin (Van) nucleic acid were employed in...
Abstract The construction of robust, modular and compact DNA machinery facilitates us to build more intelligent ingenious sensing strategies in complex biological systems. However, the performance conventional amplifiers is always impeded by their limited in-depth amplifications miscellaneously enzymatic requirements. Here, a proteinase-free reciprocal replication developed exploiting synergistic cross-activation between hybridization chain reaction (HCR) DNAzyme. DNAzyme provides an...
Human telomeres are composed of duplex TTAGGG repeats and a 3′ single-stranded DNA tail. The telomeric is protected regulated by the shelterin proteins, including protection 1 (POT1) protein that binds DNA. tail can fold into G-quadruplex (G4) Both POT1 G4 play important roles in regulating telomere length homeostasis. To date, most studies have focused on individual quadruplexes formed four repeats. Telomeric tails human cells average six times as many repeats, no structural examined...
Proline-rich tyrosine kinase 2 (PYK2) is a cytoplasmic, non-receptor implicated in multiple signaling pathways. It negative regulator of osteogenesis and considered viable drug target for osteoporosis treatment. The high-resolution structures the human PYK2 domain with different inhibitor complexes establish conventional bilobal architecture show conformational variability DFG loop. basis lack selectivity classical inhibitor, PF-431396, within FAK family explained by our structural analyses....
Parkinson's disease (PD) is an age-dependent neurodegenerative that can be caused by genetic mutations in α-synuclein (α-syn) or duplication of wild-type α-syn; PD characterized the deposition α-syn aggregates, indicating a gain toxicity from accumulation α-syn. Although major neuropathologic feature degeneration dopaminergic (DA) neurons substantia nigra, non-motor symptoms including anxiety, cognitive defect and sleep disorder precede onset motor impairment, many clinical are not DA...
Abstract Acute myeloid leukemia (AML) is a malignant hematopoietic disease and the most common type of acute in adults. The mechanisms underlying drug resistance AML are poorly understood. Activating mutations FMS-like tyrosine kinase 3 (FLT3) molecular abnormality AML. Quizartinib (AC220) potent selective second-generation inhibitor FLT3. It clinical trials for treatment relapsed or refractory FLT3-ITD–positive –negative patients as maintenance therapy. To understand to AC220, we undertook...
Human telomeres are maintained by the shelterin protein complex in which TRF1 and TRF2 bind directly to duplex telomeric DNA. How these proteins find sequences among a genome of billions base pairs how they partners form remains uncertain. Using single-molecule fluorescence imaging quantum dot-labeled TRF2, we study locate TTAGGG repeats on DNA tightropes. By virtue its basic domain performs an extensive 1D search nontelomeric DNA, whereas TRF1's is limited. Unlike stable static associations...
Abstract The oocyte cytoplasm can reprogram the somatic cell nucleus into a totipotent state, but with low efficiency. spatiotemporal chromatin organization of nuclear transfer (SCNT) embryos remains elusive. Here, we examine higher order structures mouse SCNT using low-input Hi-C method. We find that donor transforms to metaphase state rapidly after along dissolution typical 3D structure. Intriguingly, genome undergoes mitotic metaphase-like meiosis II-like transition following activation....