A5101733649- MicroRNA in disease regulation
- Epigenetics and DNA Methylation
- Genetics and Neurodevelopmental Disorders
- RNA Research and Splicing
- SARS-CoV-2 and COVID-19 Research
- RNA modifications and cancer
- Pain Mechanisms and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Animal Virus Infections Studies
- Neurogenesis and neuroplasticity mechanisms
- Single-cell and spatial transcriptomics
- CRISPR and Genetic Engineering
- Circular RNAs in diseases
- Receptor Mechanisms and Signaling
- Acupuncture Treatment Research Studies
- RNA Interference and Gene Delivery
- Healthcare and Venom Research
- Mechanical Engineering and Vibrations Research
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Mitochondrial Function and Pathology
- Neuroscience and Neuropharmacology Research
- COVID-19 Clinical Research Studies
- Renin-Angiotensin System Studies
- Chemical Synthesis and Analysis
- Immune Response and Inflammation
Jiangsu Vocational College of Medicine
2025
Harvard University
2024
Massachusetts General Hospital
2024
University of California, Los Angeles
2011-2023
Shanghai East Hospital
2021-2022
University of Cambridge
2021
Wellcome Sanger Institute
2021
Tongji University
2003-2020
Tongji Hospital
2014-2018
Neurobehavioral Systems
2014
The endoribonuclease, Dicer, is indispensable for generating the majority of mature microRNAs (miRNAs), which are posttranscriptional regulators gene expression involved in a wide range developmental and pathological processes mammalian CNS. Although functions Dicer-dependent miRNA pathways neurons oligodendrocytes have been extensively investigated, little known about role Dicer astrocytes. Here, we report effect Cre-loxP-mediated conditional deletion selectively from postnatal astroglia on...
Large-scale COVID-19 vaccinations are currently underway in many countries response to the pandemic. Here, we report, besides generation of neutralizing antibodies, consistent alterations hemoglobin A1c, serum sodium and potassium levels, coagulation profiles, renal functions healthy volunteers after vaccination with an inactivated SARS-CoV-2 vaccine. Similar changes had also been reported patients, suggesting that mimicked infection. Single-cell mRNA sequencing (scRNA-seq) peripheral blood...
Electric wheel loaders (EWLs) have emerged as a pivotal innovation in the 2020s, representing transformative shift toward high-efficiency, low-emission construction machinery. Despite their growing technological and environmental significance, systematic synthesis of advancements EWL design, energy optimization, intelligent control remains absent literature. To bridge this gap, review critically evaluates over 140 studies for comparative analysis. Building on authors’ ongoing research, paper...
Electrical stimulation in the periaqueductal gray (PAG) can inhibit dorsal horn cell responses to both innocuous and noxious cutaneous stimuli. This inhibition is believed be due release of serotonin (5-HT) into spinal cord from descending axons nucleus raphe magnus adjacent reticular formation. It still not clearly known which subtypes 5-HT receptors are involved PAG-induced inhibition. Extracellular single-unit recordings activity, combination with drug administration through a...
Aging associated cognitive decline has been linked to dampened neural stem/progenitor cells (NSC/NPCs) activities manifested by decreased proliferation, reduced propensity produce neurons, and increased differentiation into astrocytes. While gene transcription changes objectively reveal molecular alterations of undergoing various biological processes, the search for mechanisms underlying aging NSC/NPCs confronted enormous heterogeneity in cellular compositions brain complex microenvironment...
Significance The tamoxifen (TAM)-inducible CreER/LoxP system is one of the most widely used genetic tools that have enabled precise gene manipulation in distinct cell subpopulations at any specific time point and labeling types for fate mapping. We report administration TAM during early stages cortical neurogenesis inhibits neural progenitor proliferation impaired neurogenesis, dendritic morphogenesis, synaptic density, gliogenesis postnatal offspring. By employing single-cell RNA sequencing...
It has been postulated that a proneural factor, neurogenin 1 (Ngn1), simultaneously activates the neurogenic program and inhibits alternative astrogliogenic when specifying neuronal fate. While Ngn1 substantially suppresses activation of Jak-Stat pathway, underlying molecular mechanism was unknown. Here, by employing in vivo vitro approaches, we report binds to promoter brain-enriched microRNA, miR-9, its expression during neurogenesis. Subsequently, our study showed miR-9 directly targets...
Ischemic brain injury is a severe medical condition with high incidences in elderly people without effective treatment for the resulting neural damages. Using unilateral mouse stroke model, we analyze single-cell transcriptomes of ipsilateral and contralateral cortical penumbra regions to objectively reveal molecular events resolution at 4 h 1, 3, 7 days post-injury. Here, report that neurons are among first cells sense lack blood supplies by elevated expression CCAAT/enhancer-binding...
Although 5-hydroxytryptamine (5-HT) is known to be involved in the mediation of antinociception from periaqueductal gray (PAG), its mode action remains obscure. This investigation uses selective 5-HT(1A) receptor agonist and antagonist drugs both behavioral electrophysiological studies on antinociceptive mechanisms PAG rats. Intraspinal administration 8-hydroxy-dipropylaminotetralin hydrobromide (8-OH-DPAT), a agonist, by microdialysis produced dose-dependent radiant-heat paw withdrawal...
The prenatal period of cortical development is important for the establishment neural circuitry and functional connectivity brain; however, molecular mechanisms underlying this process remain unclear. Here we report that disruption actin-cytoskeletal network in developing mouse prefrontal cortex alters dendritic morphogenesis synapse formation, leading to enhanced formation fear-related memory adulthood. These effects are mediated by a brain-enriched microRNA, miR-9, through its negative...
Autoantibodies against the second extracellular loop of β(1) -adrenergic receptor (β(1) -AA) not only contribute to increased susceptibility heart failure, but also play a causative role in myocardial remodeling through their catecholamine-like effects via binding with receptor. The current study was designed determine whether -AA isolated from sera failure patients could cause TNF-α secretion murine macrophage-like cell line RAW264.7. Blood samples were collected 40 who had suffered as well...
DNA methylation and demethylation at CpG di-nucleotide sites plays important roles in cell fate specification of neural stem cells (NSCs). We have previously reported that methyltransferases, Dnmt1and Dnmt3a, serve to suppress precocious astrocyte differentiation from NSCs via astroglial lineage genes. However, whether active demethylase also participates astrogliogenesis remains undetermined. In this study, we discovered a Ten-eleven translocation (Tet) protein, Tet2, which was critically...
The mammalian central nervous system (CNS) is considered an immune privileged as it separated from the periphery by blood brain barrier (BBB). Yet, functions have been postulated to heavily influence functional state of CNS, especially after injury or during neurodegeneration. There controversy regarding whether adaptive responses are beneficial detrimental CNS repair. In this study, we utilized immunocompromised SCID mice and subjected them spinal cord (SCI). We analyzed motor function,...
Here we report that the recently discovered mammalian DNA modification N6-methyl-2’-deoxyadenosine (m6dA) is dynamically regulated in primary cortical neurons, and accumulates along promoters coding sequences within genome of activated prefrontal neurons adult C57/BI6 mice response to fear extinction learning. The deposition m6dA generally associated with increased genome-wide occupancy methyltransferase, N6amt1, this correlates learning-induced gene expression. Of particular relevance for...