A5057837314- Epigenetics and DNA Methylation
- Genetic Syndromes and Imprinting
- Genetic Associations and Epidemiology
- Genetics and Neurodevelopmental Disorders
- Genetic Mapping and Diversity in Plants and Animals
- Gene expression and cancer classification
- Tryptophan and brain disorders
- Molecular Biology Techniques and Applications
- Stress Responses and Cortisol
- RNA modifications and cancer
- Genomics and Phylogenetic Studies
- Birth, Development, and Health
- Autism Spectrum Disorder Research
- Diet and metabolism studies
- Adipose Tissue and Metabolism
- RNA and protein synthesis mechanisms
- Bioinformatics and Genomic Networks
- Animal Genetics and Reproduction
- Health, Environment, Cognitive Aging
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Neuroendocrine regulation and behavior
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Bacteriophages and microbial interactions
- Cognitive Abilities and Testing
University of Essex
2016-2025
King's College London
2008-2017
Colchester Hospital
2015
Medical Research Council
2007-2014
University of Exeter
2014
PharmacoGenetics (China)
2012
GTx (United States)
2011
Psychiatry Research Trust
2002-2011
Institute of Psychiatry and Neurology
2010
University of London
2004-2009
Abstract Background As the most stable and experimentally accessible epigenetic mark, DNA methylation is of great interest to research community. The landscape across tissues, through development in disease pathogenesis not yet well characterized. Thus there a need for rapid cost effective methods assessing genome-wide levels methylation. Illumina Infinium HumanMethylation450 (450K) BeadChip very useful addition available analysis but its complex design, incorporating two different assay...
Abstract Background Dynamic changes to the epigenome play a critical role in establishing and maintaining cellular phenotype during differentiation, but little is known about normal methylomic differences that occur between functionally distinct areas of brain. We characterized intra- inter-individual variation across whole blood multiple regions brain from donors. Results Distinct tissue-specific patterns DNA methylation were identified, with highly significant over-representation...
Studies of the major psychoses, schizophrenia (SZ) and bipolar disorder (BD), have traditionally focused on genetic environmental risk factors, although more recent work has highlighted an additional role for epigenetic processes in mediating susceptibility. Since monozygotic (MZ) twins share a common DNA sequence, their study represents ideal design investigating contribution factors to disease etiology. We performed genome-wide analysis methylation peripheral blood samples obtained from...
Given the tissue-specific nature of epigenetic processes, assessment disease-relevant tissue is an important consideration for epigenome-wide association studies (EWAS). Little known about whether easily accessible tissues, such as whole blood, can be used to address questions interindividual epigenomic variation in inaccessible brain. We quantified DNA methylation matched samples isolated from blood and 4 brain regions (prefrontal cortex, entorhinal superior temporal gyrus, cerebellum) 122...
Children's intellectual development is influenced by both genetic inheritance and environmental experiences. Breastfeeding one of the earliest such postnatal Breastfed children attain higher IQ scores than not fed breast milk, presumably because fatty acids uniquely available in milk. Here we show that association between breastfeeding moderated a variant FADS2, gene involved control acid pathways. We confirmed this gene-environment interaction two birth cohorts, ruled out alternative...
Schizophrenia is a highly heritable, neuropsychiatric disorder characterized by episodic psychosis and altered cognitive function. Despite success in identifying genetic variants associated with schizophrenia, there remains uncertainty about the causal genes involved disease pathogenesis how their function regulated.We performed multi-stage epigenome-wide association study, quantifying genome-wide patterns of DNA methylation total 1714 individuals from three independent sample cohorts. We...
Autism spectrum disorder (ASD) defines a group of common, complex neurodevelopmental disorders. Although the aetiology ASD has strong genetic component, there is considerable monozygotic (MZ) twin discordance indicating role for non-genetic factors. Because MZ twins share an identical DNA sequence, disease-discordant pairs provide ideal model examining contribution environmentally driven epigenetic factors in disease. We performed genome-wide analysis methylation sample 50 (100 individuals)...
Epigenetic processes play a key role in orchestrating transcriptional regulation during development. The importance of DNA methylation fetal brain development is highlighted by the dynamic expression de novo methyltransferases perinatal period and neurodevelopmental deficits associated with mutations methyl-CpG binding protein 2 ( MECP2 ) gene. However, our knowledge about temporal changes to epigenome has, date, been limited. We quantified genome-wide patterns at ∼400,000 sites 179 human...
There has been a steady increase in the number of studies aiming to identify DNA methylation differences associated with complex phenotypes. Many challenges epigenetic epidemiology regarding study design and interpretation have discussed detail, however there are analytical concerns that outstanding require further exploration. In this we seek address three issues. First, quantify multiple testing burden propose standard statistical significance threshold for identifying sites an outcome....
Abstract DNA methylation likely plays a role in the regulation of human stress reactivity. Here we show that genome-wide analysis blood 85 healthy individuals, locus Kit ligand gene ( KITLG ; cg27512205) showed strongest association with cortisol reactivity P =5.8 × 10 −6 ). Replication was obtained two independent samples using either N =45, =0.001) or buccal cells =255, =0.004). strongly mediates relationship between childhood trauma and discovery sample (32% mediation). Its genomic...
Alternative splicing is a post-transcriptional regulatory mechanism producing distinct mRNA molecules from single pre-mRNA with prominent role in the development and function of central nervous system. We used long-read isoform sequencing to generate full-length transcript sequences human mouse cortex. identify novel transcripts not present existing genome annotations, including mapping putative (unannotated) genes fusion incorporating exons multiple genes. Global patterns diversity are...
We present a practical description of polyethylene glycol mediated spheroplast transformation Halobacterium halobium and volcanii. This method has been applied to phage DNA transfection, plasmid transformation, with linear fragments high molecular weight genomic DNA. Efficient regeneration allows uncomplicated recovery transformed progeny. Transformations can be performed equally well using fresh or frozen cell preparations. These methods should find application in cloning, genetic fine...