A5100693585- Fungal and yeast genetics research
- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- Plant biochemistry and biosynthesis
- Polysaccharides and Plant Cell Walls
- Plant Disease Resistance and Genetics
- Mycotoxins in Agriculture and Food
- Mycorrhizal Fungi and Plant Interactions
- Microbial Natural Products and Biosynthesis
- Bacterial biofilms and quorum sensing
- Fungal Biology and Applications
- Ubiquitin and proteasome pathways
- Bacterial Genetics and Biotechnology
- Extracellular vesicles in disease
- Photosynthetic Processes and Mechanisms
- Microbial Metabolism and Applications
- RNA modifications and cancer
- Plant Pathogenic Bacteria Studies
- Antibiotic Resistance in Bacteria
- Plant Gene Expression Analysis
- Milk Quality and Mastitis in Dairy Cows
- Metabolomics and Mass Spectrometry Studies
- RNA and protein synthesis mechanisms
- MicroRNA in disease regulation
- Probiotics and Fermented Foods
Huazhong Agricultural University
2016-2025
Renmin Hospital of Wuhan University
2010-2025
Wuhan University
2010-2025
Third Affiliated Hospital of Zhengzhou University
2023-2025
Fourth Hospital of Hebei Medical University
2023-2025
Hebei Medical University
2023-2025
Beijing Technology and Business University
2023-2024
State Key Laboratory of Agricultural Microbiology
2021-2024
Sichuan Agricultural University
2024
Pharmaceutical Biotechnology (Czechia)
2024
Thiolation, a post-transcriptional modification catalyzed by Uba4-Urm1-Ncs2/Ncs6 pathway in three specific transfer RNAs (tRNAs), is conserved from yeast to humans and plays an important role enhancing codon-anticodon interaction translation efficiency. Yet, except for affecting effector secretion, its roles plant pathogenic fungi are not fully understood. Here, we used Magnaporthe oryzae as model system illustrate the vital of s2U34 on appressorium-mediated virulence. The absence tRNA...
Abstract Plant pathogenic fungi deploy secreted effectors to suppress plant immunity responses. These operate either in the apoplast or within host cells, so they are putatively glycosylated, but posttranslational regulation of their activities has not been explored. In this study, ASPARAGINE-LINKED GLYCOSYLATION3 (ALG3)-mediated N-glycosylation effector, Secreted LysM Protein1 (Slp1), was found be essential for its activity rice blast fungus Magnaporthe oryzae. ALG3 encodes an...
This research sought to examine how the physicochemical characteristics of soy globulins and different processing techniques influence gel properties yogurt. The goal was improve these rectify any texture issues in yogurt, ultimately aiming produce premium-quality plant-based In this study, investigation focused on examining impact 7S/11S, homogenization pressure, glycation modified with glucose A yogurt superior successfully developed using a 7S/11S globulin-glucose conjugate at 1:3 ratio...
In eukaryotes, N6 -methyladenosine (m6 A) is abundant on mRNA, and plays key roles in the regulation of RNA function. However, regulatory mechanisms m6 A phytopathogenic fungi are still largely unknown. Combined with biochemical analysis, MeRIP-seq RNA-seq methods, as well biological we showed that Magnaporthe oryzae MTA1 gene an orthologue human METTL4, which involved modification a critical role autophagy for fungal infection. The Δmta1 mutant reduced virulence due to blockage appressorial...
Histone acetylation is a critical epigenetic modification that regulates plant immunity. Fungal pathogens secrete effectors modulate host immunity and facilitate infection, but whether fungal have evolved directly target histone remains unknown. Here, we identified secreted protein, UvSec117, from the rice false smut fungus, Ustilaginoidea virens, as key effector can deacetylase OsHDA701 negatively broad-spectrum resistance against pathogens. UvSec117 disrupts by recruiting to nucleus...
Melatonin is a low-cost natural small indole molecule with versatile biological functions. However, melatonin's fungicidal potential has not been fully exploited, and the mechanism remains elusive. Here, we report that melatonin broadly inhibited 13 plant pathogens. In rice blast fungal pathogen Magnaporthe oryzae, growth, formation of infection-specific structures named appressoria, infection, reducing disease severity. entered cells efficiently colocalized critical mitogen-activated...
As with the majority of hemibiotrophic fungal pathogens, rice blast fungus Magnaporthe oryzae uses highly specialized infection structures called appressoria for plant penetration. Appressoria differentiated from germ tubes rely on enormous turgor pressure to directly penetrate cell, in which process lipid metabolism plays a critical role. In this study, we characterized MoPAH1 gene M. oryzae, encoding putative conserved phosphatidate phosphatase. The expression was up-regulated during...
Summary Rice false smut caused by Ustilaginoidea virens is a devastating rice ( Oryza sativa) disease worldwide. However, the molecular mechanisms underlying U . –rice interactions are largely unknown. In this study, we identified secreted protein, Uv1809, as key virulence factor. Heterologous expression of Uv1809 in enhanced susceptibility to and bacterial blight. Host‐induced gene silencing resistance , suggesting that inhibits immunity promotes infection suppresses targeting enhancing...
Trace amines (TAs) are metabolically related to catecholamine and associated with cancer neurological disorders. Comprehensive measurement of TAs is essential for understanding pathological processes providing proper drug intervention. However, the trace amounts chemical instability challenge quantification. Here, diisopropyl phosphite coupled chip two-dimensional (2D) liquid chromatography tandem triple-quadrupole mass spectrometry (LC-QQQ/MS) was developed simultaneously determine...
Summary Plant pathogenic fungi elaborate numerous detoxification strategies to suppress host reactive oxygen species (ROS), but their coordination is not well‐understood. Here, we show that Sirt5‐mediated protein desuccinylation in Magnaporthe oryzae central ROS detoxification. SIRT5 encodes a desuccinylase important for virulence via adaptation oxidative stress. Quantitative proteomics analysis identified large number of succinylated proteins targeted by Sirt5, most which were mitochondrial...
Summary Protein post‐translational modifications play critical roles in cellular processes, development and stress response. The small ubiquitin‐like modifier ( SUMO ) to proteins is one of the essential eukaryotes, but its function remains largely unknown plant pathogenic fungi. We present a comprehensive analysis combined with proteomic, molecular approaches explore sumoylation model fungal pathogen, Magnaporthe oryzae . found pathway plays key colony growth, conidia formation virulence...
Reversible protein ubiquitination plays essential roles in regulating cellular processes. Although many reports have described the functions of plant defense responses, few focused on global changes ubiquitome. To better understand regulatory rice pattern-triggered immunity (PTI), we investigated ubiquitome seedlings after treatment with two pathogen-associated molecular patterns, fungal-derived chitin or bacterial-derived flg22, using label-free quantitative proteomics. In chitin-treated...
The post-translational modification lysine 2-hydroxyisobutyrylation (Khib ) plays an important role in gene transcription, metabolism, and enzymatic activity. Khib sites have been identified rice (Oryza sativa). However, the status of proteins flowers during pathogen infection remains unclear. Here, we report a comprehensive identification -modified flowers, changes these with fungal Ustilaginoidea virens. By using tandem mass tag-based quantitative proteomics approach, 2,891 on 964 flowers....
Plant diseases cause enormous economic losses in agriculture and threaten global food security, application of agrochemicals is an important means to control crop diseases. Thus, exploring disease-resistant mechanisms synthesis highly bioactive are great importance. Here, we showed that propranolol, a phosphatidate phosphatase (Pah) inhibitor, effectively suppressed fungal growth, sporulation, sexual reproduction, plant infection on diverse plants. The MoPah1 enzyme activity the rice blast...
Actin organization is a conserved cellular process that regulates the growth and development of eukaryotic cells. It also governs virulence pathogenic fungi, such as rice blast fungus Magnaporthe oryzae, with mechanisms not yet fully understood. In previous study, we found actin-regulating kinase MoArk1 displays functions important in endocytosis actin organization, required for maintaining full M. oryzae. To understand how might function, identified capping protein homologs from oryzae...
The rise of widespread antifungal resistance fuels the need to explore new classes inhibitory molecules as potential novel inhibitors. Recently a plant natural product poacic acid (PA) was shown inhibit β-1,3-glucan synthesis, and have activity against range pathogens Saccharomyces cerevisiae. As with echinocandins, such caspofungin, PA targets synthesis cell wall has utility in treatment medically important fungi. However, human pathogenic Candida species not been explored precise mode...
Genetic studies have shown essential functions of N-glycosylation during infection the plant pathogenic fungi, however, systematic roles in fungi is still largely unknown. Biological analysis demonstrated N-glycosylated proteins were widely present at different development stages Magnaporthe oryzae and especially increased appressorium invasive hyphae. A large-scale quantitative proteomics was then performed to explore M. oryzae. total 559 N-glycosites from 355 identified quantified...
Upon fungal and bacterial pathogen attack, plants launch pattern-triggered immunity (PTI) by recognizing pathogen-associated molecular patterns (PAMPs) to defend against pathogens. Although PTI-mediated response has been widely studied, a systematic understanding of the reprogrammed cellular processes during PTI multi-omics analysis is lacking. In this study, we generated metabolome, transcriptome, proteome, ubiquitome acetylome data investigate rice (Oryza sativa) responses two PAMPs,...