A5024778178- Plant Pathogens and Fungal Diseases
- Fungal Plant Pathogen Control
- Plant Disease Resistance and Genetics
- Plant-Microbe Interactions and Immunity
- Fungal and yeast genetics research
- Mycotoxins in Agriculture and Food
- Yeasts and Rust Fungi Studies
- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Plant Physiology and Cultivation Studies
- Fungal Biology and Applications
- Plant Pathogens and Resistance
- Plant Disease Management Techniques
- Mycorrhizal Fungi and Plant Interactions
- Synthesis and Biological Evaluation
- Entomopathogenic Microorganisms in Pest Control
- Plant Pathogenic Bacteria Studies
- Garlic and Onion Studies
- Powdery Mildew Fungal Diseases
- Plant Gene Expression Analysis
- Biofuel production and bioconversion
- Plant pathogens and resistance mechanisms
- Forest Insect Ecology and Management
- Postharvest Quality and Shelf Life Management
- Fermentation and Sensory Analysis
Huazhong Agricultural University
2016-2025
Kunming Metallurgical Research Institute
2024
Anshan Hospital
2024
Ministry of Education of the People's Republic of China
2022
Clemson University
2007-2018
Nanjing Forestry University
2018
Chinese Academy of Agricultural Sciences
2018
Institute of Plant Protection
2018
Mansoura University
2016
Jiangsu Academy of Agricultural Sciences
2015
Ustilaginoidea virens (Cooke) Takah is an ascomycetous fungus that causes rice false smut, a devastating emerging disease worldwide. Here we report 39.4 Mb draft genome sequence of U. encodes 8,426 predicted genes. The has ~25% repetitive sequences have been affected by repeat-induced point mutations. Evolutionarily, close to the entomopathogenic Metarhizium spp., suggesting potential host jumping across kingdoms. possesses reduced gene inventories for polysaccharide degradation, nutrient...
Abstract Anthracnose, caused by Colletotrichum camelliae , poses a significant threat to the yield and quality of Camellia oleifera . Mitophagy, selective form autophagy, is crucial in maintaining mitochondrial intracellular homeostasis. To date, an optimized experimental system for studying mitophagy C. has yet be established, role pathogenesis remains unclear. Here, we characterized function CaEch1, homolog Magnaporthe oryza MoEch1, anthracnose fungus Our findings indicate that...
Resistance in Monilinia fructicola to demethylation inhibitor (DMI) fungicides is beginning emerge North America, but its molecular basis unknown. Two potential genetic determinants of DMI fungicide resistance including the 14alpha-demethylase gene (MfCYP51) and ATP-binding cassette transporter MfABC1, were investigated six resistant (DMI-R) sensitive (DMI-S) field isolates. No point mutations leading an amino acid change found MfCYP51 gene. The constitutive expression DMI-R isolates was...
In this study, 145 peaches and nectarines displaying typical brown rot symptoms were collected from multiple provinces in China. A subsample of 26 single-spore isolates characterized phylogenetically morphologically to ascertain species. Phylogenetic analysis internal transcribed spacer (ITS) regions 1 2, glyceraldehyde-3-phosphate dehydrogenase (G3PDH), β-tubulin (TUB2) revealed the presence three distinct Monilinia These species included fructicola, Monilia mumecola, a previously...
Two hundred and forty isolates of Botrytis cinerea were collected during the early summer 2012 2013 from strawberry greenhouses in 10 locations Hubei Province examined for sensitivity to five fungicides, most which commonly used control this fungus. High frequency resistance carbendazim (Car, 63.63%) cyprodinil (Cyp, 42.42%) was detected. Boscalid-resistant (BosR) detected first time China, whereas no fludioxonil-resistant identified. Dual diethofencarb (Die) also There six phenotypes...
Abstract Peach is a popular and important tree fruit widely produced in the world, production of high-quality peach does require management pests diseases. In this review, major diseases from China, Spain, USA are described detail for benefit producers, consultants, researchers, other interested parties. Minor concern these countries also described. Current progress on pathogen resistance to chemical classes fungicides as well current practices discussed. Specific cultural applied provide an...
High resistance to benzimidazole fungicides in Venturia carpophila is caused by the point mutation E198K of β-tubulin (TUB2) gene. Traditional methods for detection fungicide are time-consuming, which routinely based on tedious operation, reliance expensive equipment, and specially trained people. Therefore, it important establish efficient field V. make suitable management strategies ensure food safety. Based recombinase polymerase amplification (RPA) combined with CRISPR/Cas12a, a rapid...
Here, we describe a method that can be used to evaluate the function of predicted signal peptides. This utilizes yeast Saccharomyces cerevisiae YTK12 strain and pSUC2 vector in which with fused peptide is transformed into yeast. The peptides evaluated by using different selective media color reaction. In this protocol, provide detailed description manipulation order implement easily.
Summary Rice false smut disease is an increasing threat to rice production in the world. Despite of best efforts, research for infection fungus has yielded equivocal and conflicting results about where how initiated developed. Here we show a stepwise pattern sophisticated regulation during this process. Initial occurred on filaments, which prevented mature pollen thus blocked pollination. In following days, pathogen invaded stigmas styles, occasionally ovaries. Expression analysis indicated...
Histone acetylation is a critical epigenetic modification that regulates plant immunity. Fungal pathogens secrete effectors modulate host immunity and facilitate infection, but whether fungal have evolved directly target histone remains unknown. Here, we identified secreted protein, UvSec117, from the rice false smut fungus, Ustilaginoidea virens, as key effector can deacetylase OsHDA701 negatively broad-spectrum resistance against pathogens. UvSec117 disrupts by recruiting to nucleus...
Colletotrichum species are important plant pathogens, causing anthracnose in virtually every crop grown throughout the world. However, little is known about that infect watermelon. A total of 526 strains were isolated from diseased watermelon samples eight major growing provinces China. Phylogenetic analyses using seven loci (ITS, gadph, chs-1, his3, act, tub2, and gs) coupled with morphology 146 representative isolates showed they belonged to 12 Colletotrichum, including C. aenigma,...
Few fungicides are effective against anthracnose, caused by Colletotrichum spp., and emerging resistance makes the search for chemical alternatives more relevant. Isolates of acutatum species complex were collected from South Carolina Georgia peach orchards phylogenetic analysis combined internal transcribed spacer region, glyceraldehyde-3-phosphate dehydrogenase, β-tubulin gene sequences separated isolates into C. nymphaeae fioriniae. The sensitivity these three other previously reported...
The fungicide resistance profiles to seven chemical classes of fungicides were investigated in 198 Botrytis cinerea isolates from five blackberry fields and 214 B. 10 strawberry North South Carolina. Populations tended have a single dominant, location-specific profile that consisted multiple sprayed weekly with site-specific fungicides. most prevalent thiophanate-methyl, pyraclostrobin, boscalid. found conventional boscalid, cyprodinil. A statistical model revealed multifungicide patterns...
Abstract BACKGROUND The dicarboximide fungicide dimethachlone has been widely used in China for more than 12 years to control the Sclerotinia stem rot caused by sclerotiorum disease. First signs of resistance field are reported at low frequency. In this study, four resistant isolate/mutants were explore still unknown mechanisms leading resistance. RESULTS had significantly higher EC 50 values compared with sensitive isolates. Cross‐resistance was confirmed between and procymidone, iprodione...
Resistance to multiple chemical classes of fungicides in Botrytis cinerea isolates from eastern United States strawberry fields is common and strategies control them are needed. In this study, we compared fitness competitive ability eight sensitive (S), resistant five or six but not phenylpyrroles (5CCR), seven including (6CCR/MDR1h). The latter included the MDR1h phenotype due overexpression atrB based on Δ497V/L mrr1. 6CCR/MDR1h grew more slowly at 4°C potato dextrose agar, both 5CCR were...
The post-translational modification lysine 2-hydroxyisobutyrylation (Khib ) plays an important role in gene transcription, metabolism, and enzymatic activity. Khib sites have been identified rice (Oryza sativa). However, the status of proteins flowers during pathogen infection remains unclear. Here, we report a comprehensive identification -modified flowers, changes these with fungal Ustilaginoidea virens. By using tandem mass tag-based quantitative proteomics approach, 2,891 on 964 flowers....
Abstract Background Peach diseases can cause severe yield reduction and decreased quality for peach production. Rapid accurate detection identification of is great importance. Deep learning has been applied to detect using imaging data. However, disease image data difficult collect samples are imbalance. The popular deep networks perform poor this issue. Results This paper proposed an improved Xception network named as L2MXception which ensembles regularization term L2-norm mean. With the...
is regarded as one of the 10 most important genera plant pathogens in world. It causes diseases a wide range economically plants, including peaches. China largest producer peaches world but little known about
Sterol demethylation inhibitor (DMI) fungicide resistance in isolates of Monilinia fructicola from Georgia has been linked to overexpression the MfCYP51 gene and a corresponding 65-bp genetic element 'Mona' inserted upstream region MfCYP51. In this study, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was developed detect Mona element. Fourteen DMI fungicide-resistant (DMI-R) six fungicide-sensitive (DMI-S) Georgia, DMI-R 11 DMI-S South Carolina, seven...
Brown rot of peach caused by Monilinia fructicola can cause considerable preharvest and postharvest losses in China. Fungicides are increasingly utilized to minimize such losses. Eighty isolates M. were collected from commercial orchards located five provinces China the sensitivity carbendazim, azoxystrobin, tebuconazole, boscalid was determined. Resistance carbendazim detected only Yunnan province 15 16 isolates. Characterization carbendazim-resistant revealed stable resistance, no fitness...
Field isolates of Alternaria alternata from peach were previously characterized for their sensitivity to succinate dehydrogenase inhibitor (SDHI) fungicides and the underlying molecular basis resistance was determined. In present study, we report that resistant SDHI fungicide boscalid, regardless genotype, also pyraclostrobin thiophanate-methyl. Resistance due G143A mutation in cytochrome b thiophanate-methyl 167Y β-tubulin. Representatives two most commonly isolated genotypes, H277Y sdh...