A5004473247- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Entomopathogenic Microorganisms in Pest Control
- Insect symbiosis and bacterial influences
- Osteoarthritis Treatment and Mechanisms
- Insect and Arachnid Ecology and Behavior
- Cancer-related molecular mechanisms research
- Supramolecular Self-Assembly in Materials
- MicroRNA in disease regulation
- Insect Resistance and Genetics
- Single-cell and spatial transcriptomics
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- 3D Printing in Biomedical Research
- RNA Research and Splicing
- Viral Infections and Immunology Research
- Renal and related cancers
- Nanocomposite Films for Food Packaging
- Protease and Inhibitor Mechanisms
- Microbial Natural Products and Biosynthesis
- Proteoglycans and glycosaminoglycans research
- Tissue Engineering and Regenerative Medicine
- Silk-based biomaterials and applications
- RNA regulation and disease
- Viral Infectious Diseases and Gene Expression in Insects
Chinese Academy of Sciences
2014-2024
Center for Excellence in Molecular Plant Sciences
2019-2024
University of Chinese Academy of Sciences
2022-2024
Zunyi Medical University
2015-2024
Northwest A&F University
2023-2024
First Affiliated Hospital of Wannan Medical College
2024
Bohai University
2024
Second Affiliated Hospital of Zhengzhou University
2019-2021
Guangzhou Institutes of Biomedicine and Health
2010-2019
Sichuan University
2009-2018
Abstract Background Bacterial transfers from plants to insect herbivore guts have been well investigated. However, bacterial exchanges between plant phyllospheres and cuticles remain unclear, as does their related biological function. Results Here, we report that the cuticular loads of silkworm larvae quickly increased after molting feeding on white mulberry ( Morus alba ) leaves. The isolation examination bacteria identified one bacterium Mammaliicoccus sciuri could completely inhibit spore...
Pig cloning by somatic cell nuclear transfer (SCNT) remains extremely inefficient, and many cloned embryos undergo abnormal development. Here, profiling transcriptome expression, we observed dysregulated chromosome-wide gene expression in every chromosome identified a considerable number of genes that are aberrantly expressed the embryos. In particular, XIST, long non-coding RNA gene, showed high ectopic We also proved nullification XIST donor cells can normalize aberrant enhance long-term...
In contrast to the well-characterized gut microbiomes, composition and function of insect body-surface microbiotas are still elusive highly underexplored. Here we report dynamic features Drosophila melanogaster surface microbiomes. It was found that microbiomes assembled on fly surfaces could defend insects against fungal parasitic infections. The substantial increase bacterial loads occurred within 10 days eclosion, especially expansion Gilliamella species. culturable bacteria such as...
Entomopathogenic fungi (EPF) infect insects by landing on and penetrating cuticles. Emerging evidence has shown that, prior to the invasion of insects, fungal cells have battle overcome diverse challenges, including host behavioral defenses, colonization resistance mediated ectomicrobiotas, recognition, generation enough penetration pressure. The ascomycete EPF such as Metarhizium Beauveria can thus produce adhesive proteins and/or exopolysaccharide mucilage tightly glue Producing...
Abstract The promising potential of a RAD‐16 self‐assembly‐peptide hydrogel as scaffold for tissue‐engineered cartilage was investigated. Within 3 weeks in vitro culture, chondrocytes within the produced high amount GAG and type‐II collagen, which are components cartilage‐specific extracellular matrix (ECM). With culture time increased, toluidine‐blue staining immuno‐histochemistry collagen chondrocytes‐hydrogel composites became more intense. Analysis gene expression ECM molecules also...
Human pluripotent stem cells (hPSCs) exhibit very limited contribution to interspecies chimeras. One explanation is that the conventional hPSCs are in a primed state and so unable form chimeras pre-implantation embryos. Here, we show undergo rapid apoptosis when injected into mouse While, forced-expression of BMI1, polycomb factor overcomes enables integrate embryos subsequently contribute with both embryonic extra-embryonic tissues. In addition, BMI1 also other species, such as rabbit pig....
The indolizidine alkaloid swainsonine (SW) is a deadly mycotoxin to livestock that can be produced by different plant-associated fungi, including the endophytic entomopathogenic fungi Metarhizium species. SW biosynthetic gene cluster has been identified but genetic mechanism of biosynthesis remains obscure. To unveil pathway, we performed deletions in M. robertsii, heterologous expression core gene, substrate feedings, mass spectrometry, and bioassay analyses this study. It was unveiled via...
Abstract Background Ovarian cancer is a highly aggressive malignant disease in gynecologic cancer. It an urgent task to develop three-dimensional (3D) cell models vitro and dissect the progression-related drug resistance mechanisms vivo. In present study, RADA16-I peptide has reticulated nanofiber scaffold networks hydrogel, which utilized robust 3D culture of high metastatic human ovarian HO-8910PM line accompanied with counterparts Matrigel collagen I. Results Consequently, cells were...
Generation of induced pluripotent stem cells from somatic using defined factors has potential relevant applications in regenerative medicine and biology. However, this promising technology remains inefficient time consuming. We have devised a serum free culture medium termed iSF1 that facilitates the generation mouse cells. This optimization is sensitive to presence Myc reprogramming factors. Moreover, we could reprogram meningeal only two Oct4/Klf4. Therefore, represents basal may be used...
Abstract We evaluated the hemostatic efficacy of a biological self‐assembling peptide RADA16‐I in rat kidney injury model. Adult male rats were randomized into five groups: sham operation (no renal excision), no agent (control), commercially available gelatin sponge (Gelfoam), 1% RADA16‐I, and 2% RADA16‐I. After left partial nephrectomy, anesthetized animal was anticoagulated using 300 IU · kg −1 heparin, topical applied to injury. Blood loss mean arterial pressure (MAP) recorded. As case...
The nortriterpenoid helvolic acid (HA) has potent antibiotic activities and can be produced by different fungi, yet HA function remains elusive. Here, we report the chemical biology of production in insect pathogen Metarhizium robertsii. After deletion core oxidosqualene cyclase gene Metarhizium, survival rates were significantly increased compared to those insects treated with wild type gene-rescued strain during topical infections but not injection assays bypass cuticles. Further...
In the present study, expression of Beclin 1 in osteoarthritis (OA) cartilage tissue was investigated, and also its role proliferation, apoptosis matrix metalloproteinases (MMPs) chondrocytes obtained from patients with OA. OA patients, age- sex-matched controls, detected by immunohistochemistry, semi-quantitative polymerase chain reaction western blotting. Chondrocytes were divided into control 1-overexpressed groups. After transfection for 48, 72 96 h, cell viability, apoptosis,...
Many prokaryotic and eukaryotic proteins contain domains of unknown function (DUFs). A DUF3129 family is widely encoded in the genomes fungal pathogens. few studies plant insect pathogens indicated that genes are required for penetration host cuticles with an unclear mechanism. We found a varied number present different species evolutionarily diverged from each other at inter- intra-specific levels. By using pathogenic fungus Metarhizium robertsii as model, we performed experiments seven by...