A5085975071- Pluripotent Stem Cells Research
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- CRISPR and Genetic Engineering
- Enzyme Production and Characterization
- interferon and immune responses
- Bacterial Genetics and Biotechnology
- RNA Interference and Gene Delivery
- Extracellular vesicles in disease
- Enzyme Catalysis and Immobilization
- Bacteriophages and microbial interactions
- Cytokine Signaling Pathways and Interactions
- RNA and protein synthesis mechanisms
- Renal and related cancers
- Diabetes and associated disorders
- Phytase and its Applications
- Biofuel production and bioconversion
- Lymphatic System and Diseases
- Cancer-related molecular mechanisms research
- Congenital heart defects research
- Glycosylation and Glycoproteins Research
- Adipokines, Inflammation, and Metabolic Diseases
- Angiogenesis and VEGF in Cancer
- MicroRNA in disease regulation
- Wound Healing and Treatments
Shenzhen Zhongshan Urological Hospital
2022-2025
Tianjin Institute of Industrial Biotechnology
2013-2024
Chinese Academy of Sciences
2013-2024
Nanjing Medical University
2024
Kangda College of Nanjing Medical University
2024
Guangdong University of Technology
2023
Shenzhen University
2017-2022
Shenzhen University Health Science Center
2018-2022
Shanghai Institute of Materia Medica
2020
Binzhou People's Hospital
2015-2019
Induced pluripotent stem cell (iPS) technology appears to be a general strategy generate cells from any given mammalian species. So far, iPS have been reported for mouse, human, rat, and monkey. These four species also established embryonic (ESC) lines that serve as the gold standard pluripotency comparisons. Attempts made porcine ESC by various means without success. Here we report successful generation of fibroblasts isolated Tibetan miniature pig using modified protocol. The resulting...
Mesenchymal stem/stromal cells (MSCs) represent a heterogeneous cell population distributed throughout various tissues, demonstrating remarkable adaptability to microenvironmental cues and holding immense promise for disease treatment. However, the inherent diversity within MSCs often leads variability in therapeutic outcomes, posing challenges clinical applications. To address this heterogeneity, purification of MSC subpopulations through marker-based isolation has emerged as promising...
Antimicrobial peptides (AMPs) represent a class of natural that form part the innate immune system, and this kind ‘nature's antibiotics’ is quite promising for solving problem increasing antibiotic resistance. In view this, it highly desired to develop an effective computational method accurately predicting novel AMPs because can provide us with more candidates useful insights drug design. study, new was implemented by integrating sequence alignment feature selection method. It observed...
The umbilical cord and placenta are extra-embryonic tissues of particular interest for regenerative medicine. They share an early developmental origin a source vast amounts cells with multilineage differentiation potential that poorly immunogenic without controversy. Moreover, these likely exempt from incorporated mutations when compared juvenile or adult donor such as skin fibroblasts keratinocytes. Here we report the efficient generation induced pluripotent stem (iPSCs) mesenchymal matrix...
Directed hepatocyte differentiation from human induced pluripotent stem cells (iPSCs) potentially provides a unique platform for modeling liver genetic diseases and performing drug-toxicity screening in vitro. Wilson's disease is caused by mutations the ATP7B gene, whose product transporter protein responsible coordinated copper export into bile blood. Interestingly, spectrum of vast can influence clinical presentation (a variable hepatic neural manifestations), though reason not well...
The recent discovery of induced pluripotent stem cell (iPSC) technology provides an invaluable tool for creating in vitro representations human genetic conditions. This is particularly relevant those diseases that lack adequate animal models or where the species comparison difficult, e.g. imprinting such as neurogenetic disorder Prader-Willi syndrome (PWS). However, reports have unveiled transcriptional and functional differences between iPSCs embryonic cells cases are attributable to...
Aging | doi:10.18632/aging.100277. Jenny CY Ho, Ting Zhou, Wing-Hon Lai, Yinghua Huang, Yau-Chi Chan, Xingyan Li, Navy LY Wong, Yanhua Ka-Wing Au, Dongsheng Guo, Jianyong Xu, Chung-Wah Siu, Duanqing Pei, Hung-Fat Tse, Miguel Angel Esteban
Significance Statement Although mesenchymal stem cells might have potential for treating SLE, their immunoregulatory plasticity renders therapeutic effects unpredictable. The authors genetically modified to overexpress IL-37—a protein with immunosuppressive activity—and assessed the cells’ on immune suppression in vitro , as well of transplanting such into a mouse model SLE. Mice transplanted IL-37–overexpressing displayed improved survival and reduced signs SLE compared controls. Expression...
The ciliary hypothesis for cystic renal diseases postulates that most of these conditions result from abnormalities in the primary cilium, a microtubule-based structure acts as sensor extracellular cues. Inactivation von Hippel-Lindau (VHL) tumor suppressor gene predisposes to cysts and clear cell carcinoma. VHL plays critical role formation cilia kidney epithelium, but underlying mechanisms are poorly understood. Here, we demonstrate inactivation induces HEF1/Cas-L/NEDD9 Aurora kinase A via...
Pullulanase is a debranching enzyme that specifically hydrolyzes the α-1,6 glycosidic linkage of α-glucans, and has wide industrial applications. Here, we report structural functional studies new thermostable pullulanase from Anoxybacillus sp. LM18-11 (PulA). Based on hydrolysis products, PulA was classified as type I pullulanase. It showed maximum activity at 60°C pH 6.0. Kinetic study specific Km for pullulan are 750 U mg−1 16.4 μmol L−1, respectively. half-life 48 h 60°C. The remarkable...
Generation of induced pluripotent stem cells from somatic using defined factors has potential relevant applications in regenerative medicine and biology. However, this promising technology remains inefficient time consuming. We have devised a serum free culture medium termed iSF1 that facilitates the generation mouse cells. This optimization is sensitive to presence Myc reprogramming factors. Moreover, we could reprogram meningeal only two Oct4/Klf4. Therefore, represents basal may be used...
C-C chemokine receptor type 5 (CCR5) is a major co-receptor for the entry of human immunodeficiency virus type-1 (HIV-1) into target cells. Human hematopoietic stem cells (hHSCs) with naturally occurring CCR5 deletions (Δ32) or artificially disrupted have shown potential curing acquired syndrome (AIDS). However, Δ32 donors are scarce, heterologous bone marrow transplantation not exempt risks, and genetic engineering autologous hHSCs trivial. Here, we locus embryonic (hESCs) induced...
Large-scale genome-wide association studies have reported EPHA1 rs11767557 variant to be associated with Alzheimer’s disease (AD) risk in the European population. However, it is still unclear how this functionally contributes underlying pathogenesis. The located approximately 3 kb upstream of gene. We think that may modify expression nearby genes such as and further cause AD risk. Until now, potential between has not been previous studies. Here, we evaluate using multiple large-scale eQTLs...
pWB980 derived from pUB110 is a promising expression vector in Bacillus for its high copy number and stability. However, the low transformation rate of recombinant plasmids to wild cells limited application it. On basis pWB980, constructing an E. coli-B. subtilis shuttle plasmid could facilitate cells. Because insertion site coli replication origin sequence (ori) not unique order investigate best site, eight (pUC980-1 ~ pUC980-8) containing all possible sites directions were constructed.The...
Recent pre-clinical and clinical studies have suggested that endogenous cardiospheres (eCS) are potentially safe effective for cardiac regeneration following myocardial infarction (MI). Nevertheless the preparation of autologous eCS requires invasive biopsy with limited yield. We describe a novel approach to generate induced (iCS) from adult skin fibroblasts via somatic reprogramming. After infection Sox2, Klf4, Oct4, iCS were generated mouse treated Gsk3β inhibitor-(2'Z,3'E)-...
Abstract Objectives Mesenchymal stem cells (MSCs) have been intensively investigated as to their therapeutic potentials. However, the full chemical-defined medium supporting isolation and expansion of human MSCs has not developed yet. Materials methods Here, we medium, NBVbe via RNA sequencing, bioinformatic analysis, growth factor screening. Results The contains N2B27 with BSA (bovine serum albumin) replaced by recombinant albumin, bFGF (basic fibroblast factor), vitamin C, EGF (epidermal...
Abstract Background Mesenchymal stromal/stem cells (MSCs) have been intensively investigated in both pre-clinical and clinical studies. However, the therapeutic efficacy varies resulting from heterogenicity of MSCs. Therefore, purifying specific MSC subpopulation with specialized function is necessary for their applications. Methods The large-scale RNA sequencing analysis was performed to identify potential cell markers mouse Then, immune suppression activities purified were assessed vitro...
An increasing number of studies have reported the close relation hemoglobin glycation index (HGI) with metabolism, inflammation, and disease prognosis. However, prognostic relationship between HGI patients sepsis remains unclear. Thus, this study aimed to analyze association all-cause mortality in using data from MIMIC-IV database. In study, 2605 were retrospectively analyzed. The linear regression equation was established by incorporating glycated (HbA1c) fasting plasma glucose levels....