A5101400248- Organometallic Compounds Synthesis and Characterization
- Inorganic Chemistry and Materials
- Synthesis and characterization of novel inorganic/organometallic compounds
- Enzyme Structure and Function
- Plant biochemistry and biosynthesis
- Ion channel regulation and function
- Thermal and Kinetic Analysis
- Protein Structure and Dynamics
- Crystallization and Solubility Studies
- Neuroscience and Neuropharmacology Research
- Cardiac electrophysiology and arrhythmias
- Biochemical and Molecular Research
- X-ray Diffraction in Crystallography
- Creativity in Education and Neuroscience
- Polyoxometalates: Synthesis and Applications
- Chemical Thermodynamics and Molecular Structure
- Drug Transport and Resistance Mechanisms
- Synthesis and Characterization of Heterocyclic Compounds
- Material Properties and Applications
- Education, Achievement, and Giftedness
- Perfectionism, Procrastination, Anxiety Studies
- Trace Elements in Health
- Neuroscience, Education and Cognitive Function
- Synthesis and biological activity
- Food Quality and Safety Studies
Imperial College London
2023
Washington University in St. Louis
2016-2019
Tianjin Institute of Industrial Biotechnology
2012-2014
Chinese Academy of Sciences
2012-2013
Beijing Institute of Technology
2006
Abstract Copper (Cu) is an essential trace element for growth and development abnormal Cu levels are associated with anemia, metabolic disease cancer. Evolutionarily conserved from fungi to humans, the high-affinity + transporter Ctr1 crucial both dietary uptake peripheral distribution, yet mechanisms selective permeation of potentially toxic ions across cell membranes unknown. Here we present X-ray crystal structures Salmo salar in -free -bound states, revealing a homo-trimeric -selective...
Inward rectifier potassium (Kir) channel activity is controlled by plasma membrane lipids. Phosphatidylinositol-4,5-bisphosphate (PIP2) binding to a primary site required for opening of classic inward Kir2.1 and Kir2.2 channels, but interaction bulk anionic phospholipid (PL−) with distinct second high PIP2 sensitivity. Here we show that introduction lipid-partitioning tryptophan at the (K62W) generates sensitivity, even in absence PL−. Furthermore, high-resolution x-ray crystal structures...
Pullulanase is a debranching enzyme that specifically hydrolyzes the α-1,6 glycosidic linkage of α-glucans, and has wide industrial applications. Here, we report structural functional studies new thermostable pullulanase from Anoxybacillus sp. LM18-11 (PulA). Based on hydrolysis products, PulA was classified as type I pullulanase. It showed maximum activity at 60°C pH 6.0. Kinetic study specific Km for pullulan are 750 U mg−1 16.4 μmol L−1, respectively. half-life 48 h 60°C. The remarkable...
Potassium ion conduction through open potassium channels is essential to control of membrane potentials in all cells. To elucidate the conformation and hence mechanism K + classic inward rectifier Kir2.2, we introduced a negative charge (G178D) at crossing point inner helix bundle, location ligand-dependent gating. This “forced open” mutation generated that were active even complete absence phosphatidylinositol-4,5-bisphosphate (PIP 2 ), an otherwise ligand for Kir channel opening. Crystal...
Barrel-shaped: The enzyme MoeO5 catalyzes the transfer of C15 moiety farnesyl pyrophosphate to 2-hydroxy group 3-phosphoglycerate give 2-(Z,E)-farnesyl-3-phosphoglycerate (FPG; ligand in center shown structure). X-ray crystallographic structures showed that forms a triose-phosphate-isomerase barrel structure and binds FPG curved pocket, mainly as result its long λ3 loop (magenta picture).
Well structured: As a new triose phosphate isomerase (TIM) barrel-fold prenyl transferase, PcrB catalyzes the production of heptaprenylglyceryl from heptaprenyl diphosphate and glycerol-1-phosphate. Crystal structures Bacillus subtilis Staphylococcus aureus in complex with ligands were solved, together site-directed mutagenesis bioinformatics analyses, clearly reveal catalytic mechanism enzyme. service to our authors readers, this journal provides supporting information supplied by authors....
Potassium ion conduction through open potassium channels is essential to control of membrane potentials in all cells. To elucidate the conformation and hence mechanism K + classical inward rectifier Kir2.2, we introduced a negative charge (G178D) at crossing point inner helix bundle (HBC), location ligand-dependent gating. This ‘forced open’ mutation generated that were active even complete absence phosphoinositol-4,5-bisphosphate (PIP 2 ), an otherwise ligand for Kir channel opening....
Fassförmig: Das Enzym MoeO5 katalysiert den Transfer der C15-Einheit von Farnesylpyrophosphat an die 2-Hydroxygruppe 3-Phosphoglycerat unter Bildung 2-(Z,E)-Farnesyl-3-phosphoglycerat (FPG; Ligand im Zentrum gezeigten Struktur). Röntgenkristallstrukturen zeigen, dass Fass-Struktur einer Triosephosphatisomerase hat, und das FPG aufgrund des langen λ3-Loops (magenta Bild) in gebogenen Tasche bindet. Detailed facts of importance to specialist readers are published as ”Supporting Information”....
YisP is an enzyme involved in the pathway of biofilm formation bacteria and predicted to possess squalene synthase activity. A BlastP search using protein sequence from Bacillus subtilis subsp. strain 168 shows that it shares 23% identity with dehydrosqualene Staphylococcus aureus. The B. was expressed Escherichia coli recombinant purified crystallized. crystals, which belong orthorhombic space group P2(1)2(1)2(1), unit-cell parameters a = 43.966, b 77.576, c 91.378 Å, were obtained by...
This study presents a novel framework for compensating temperature effects in composite structures with different thicknesses, based on both theoretical and experimental investigations. The developed method proposes compensation factors that can be scalable to panels of thicknesses made from the same material. In analysis, relationship between group velocity amplitude versus frequencies are determined by semi-analytical finite element (SAFE) tuning curve models. then examined through...