A5100375103- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Renal and related cancers
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- 3D Printing in Biomedical Research
- DNA Repair Mechanisms
- Virus-based gene therapy research
- Mitochondrial Function and Pathology
- Single-cell and spatial transcriptomics
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- Genomics and Chromatin Dynamics
- Viral Infectious Diseases and Gene Expression in Insects
- RNA modifications and cancer
- Cancer-related gene regulation
- Gastrointestinal motility and disorders
- Helicobacter pylori-related gastroenterology studies
- CAR-T cell therapy research
- Neuroinflammation and Neurodegeneration Mechanisms
- Gastroesophageal reflux and treatments
- Neuroscience and Neural Engineering
- Genomics and Phylogenetic Studies
- Microplastics and Plastic Pollution
- Cancer Research and Treatments
Guangzhou Medical University
2013-2025
Guangzhou Institutes of Biomedicine and Health
2015-2025
Chinese Academy of Sciences
2015-2025
Dalian Medical University
2024-2025
First Affiliated Hospital of Dalian Medical University
2024-2025
Institute of Zoology
2024-2025
Guangzhou Regenerative Medicine and Health Guangdong Laboratory
2018-2024
University of Hong Kong
2024
Xiamen University
2016-2024
Shantou University
2024
Somatic cells can be reprogrammed into induced pluripotent stem (iPSCs) by defined factors. The low efficiency of reprogramming and genomic integration oncogenes viral vectors limited the potential application iPSCs. Here we report that Lithium (Li), a drug used to treat mood disorders, greatly enhances iPSC generation from both mouse embryonic fibroblast human umbilical vein endothelial cells. Li facilitates with one (Oct4) or two factors (OS OK). effect on promoting only partially depends...
Abstract Accumulating evidence implicates that herpes simplex virus type 1 (HSV-1) has been linked to the development and progression of Alzheimer’s disease (AD). HSV-1 infection induces β-amyloid (Aβ) deposition in vitro vivo, but effect precise mechanism remain elusive. Here, we show brains transgenic 5xFAD mice resulted accelerated Aβ deposition, gliosis, cognitive dysfunction. We demonstrate induced recruitment microglia viral core trigger microglial phagocytosis HSV-GFP-positive...
Abstract Cellular senescence is characterized by a decrease in protein synthesis, although the underlying processes are mostly unclear. Chemical modifications to transfer RNAs (tRNAs) frequently influence tRNA activity, which crucial for translation. We describe how N7-methylguanosine (m7G46) methylation, catalyzed METTL1-WDR4, regulates translation and influences phenotypes. Mettl1/Wdr4 m7G gradually diminish with aging. A METTL1 causes reduction tRNAs, especially those modification, via...
Proteins possess distinct intracellular roles allowing them to have vast therapeutic applications. However, due poor cellular permeability and fragility of most proteins, delivery native, active proteins is challenging. We describe a biomimetic protein vehicle which degradable upon the digestion by furin, ubiquitous protease, release encapsulated cargos. were in nanosized matrix prepared with monomers bisacrylated peptide cross-linker can be specifically recognized cleaved furin. Release was...
Abstract The transition of embryonic stem cell (ESC) pluripotency to differentiation is accompanied by an expansion mRNA and proteomic diversity. Post-transcriptional regulation ESCs critically governed type-specific splicing. However, little known about the splicing factors molecular mechanisms directing ESC early lineage differentiation. Our study identifies RNA binding motif protein 24 (Rbm24) as a key regulator that plays essential role in controlling post-transcriptional networks during...
Highlights•Jdp2, Jhdm1b, Mkk6, Glis1, Nanog, Esrrb, and Sall4 (7F) convert MEFs into iPSCs•RNA-seq ATAC-seq reveal a distinct path for 7F reprogramming•7F cooperate to open close chromatin during activate TF network induce pluripotencySummaryReprogramming somatic cells pluripotency by Oct4, Sox2, Klf4, Myc represent paradigm cell fate determination. Here, we report combination of Jdp2, Essrb, that reprogram mouse embryonic fibroblasts or chimera competent induced pluripotent stem (iPSCs)...
Cell fate decision involves rewiring of the genome, but remains poorly understood at chromatin level. Here, we report that remodeling complex NuRD participates in closing open early phase somatic reprogramming. Sall4, Jdp2, Glis1 and Esrrb can reprogram MEFs to iPSCs efficiently, only Sall4 is indispensable capable recruiting endogenous components NuRD. Yet knocking down reduces reprogramming modestly, contrast disrupting known Sall4-NuRD interaction by mutating or deleting interacting motif...
In adult cardiomyocytes (CMs), the Na+/Ca2+ exchanger (NCX) is a well-defined determinant of Ca2+ homeostasis. Developmentally, global NCX knockout in mice leads to abnormal myofibrillar organization, electrical defects, and early embryonic death. Little known about expression function human heart development. Self-renewable, pluripotent stem cells (hESCs) can serve as an excellent experimental model. However, hESC-derived CMs are highly heterogeneous. A stably lentivirus-transduced hESC...
The static magnetic fields (SMFs) impact on biological systems, induce a variety of responses, and have been applied to the clinical treatment diseases. However, underlying mechanisms remain largely unclear. In this report, by using human mesenchymal stem cells (MSCs) as model, we investigated effect SMFs at molecular cellular level. We showed that SMF exposure promotes MSC proliferation activates expression transcriptional factors such FOS (Fos Proto-Oncogene, AP-1 Transcription Factor...
Generation of induced pluripotent stem cells from somatic using defined factors has potential relevant applications in regenerative medicine and biology. However, this promising technology remains inefficient time consuming. We have devised a serum free culture medium termed iSF1 that facilitates the generation mouse cells. This optimization is sensitive to presence Myc reprogramming factors. Moreover, we could reprogram meningeal only two Oct4/Klf4. Therefore, represents basal may be used...
The barred knifejaw (Oplegnathus fasciatus), a member of the Oplegnathidae family Centrarchiformes, is commercially important rocky reef fish native to East Asia. Oplegnathus fasciatus has become an fishery resource for offshore cage aquaculture and stocking marine ranching in China, Japan, Korea. Recently, sexual dimorphism growth with neo-sex chromosome widespread biotic diseases O. have been increasing concern industry. However, adequate genome resources gaining insight into...
Abstract Background Thymidine analogs have long been recognized for their ability to randomly incorporate into DNA. However, the precise mechanisms through which thymidine facilitate cell fate transition remains unclear. Results Here, we discovered a strong correlation between dosage dependence of and overcome reprogramming barrier. The extraembryonic endoderm (XEN) state seems be cell's selective response DNA damage repair (DDR), offering shortcut barriers. Meanwhile, found that homologous...
Abstract Background Stem cell‐based therapy (SCT) is an important component of regenerative that brings hope to many patients. After decades development, SCT has made significant progress in the research various diseases, and market size also expanded significantly. The transition from small‐scale, customized experiments routine clinical practice requires assistance standards. Many countries international organizations around world have developed corresponding standards, which effectively...