A5053863938- Fungal Biology and Applications
- Microbial Natural Products and Biosynthesis
- Phytochemicals and Antioxidant Activities
- Tea Polyphenols and Effects
- Entomopathogenic Microorganisms in Pest Control
- Insect Utilization and Effects
- Chemical synthesis and alkaloids
- Phytochemical compounds biological activities
- Food Quality and Safety Studies
- Natural product bioactivities and synthesis
- Chromatography in Natural Products
- Microbial Metabolism and Applications
- Traffic control and management
- Insect Resistance and Genetics
- Insect Pest Control Strategies
- Free Radicals and Antioxidants
- Phytochemistry and Bioactivity Studies
- Tryptophan and brain disorders
- Marine Sponges and Natural Products
- Phytochemistry and Biological Activities
- Medicinal Plants and Neuroprotection
- Lanthanide and Transition Metal Complexes
- Tannin, Tannase and Anticancer Activities
- Plant biochemistry and biosynthesis
- Seaweed-derived Bioactive Compounds
Anhui Agricultural University
2015-2024
Southwest Jiaotong University
2023
Tea Research Institute
2015
Hefei University
2015
China West Normal University
2008
Friedrich Schiller University Jena
2002
Mycoviruses are widely present in all major groups of fungi but those entomopathogenic Metarhizium spp. remain understudied. In this investigation, a novel double-stranded (ds) RNA virus is isolated from majus and named partitivirus 1 (MmPV1). The complete genome sequence MmPV1 comprises two monocistronic dsRNA segments (dsRNA 2), which encode an RNA-dependent polymerase (RdRp) capsid protein (CP), respectively. classified as new member the genus Gammapartitivirus family Partitiviridae based...
The indolizidine alkaloid swainsonine (SW) is a deadly mycotoxin to livestock that can be produced by different plant-associated fungi, including the endophytic entomopathogenic fungi Metarhizium species. SW biosynthetic gene cluster has been identified but genetic mechanism of biosynthesis remains obscure. To unveil pathway, we performed deletions in M. robertsii, heterologous expression core gene, substrate feedings, mass spectrometry, and bioassay analyses this study. It was unveiled via...
Green tea polyphenols show positive effects on human health and longevity. However, knowledge of the antiaging properties green is limited to major catechin epigallocatechin gallate (EGCG). The search for new ingredients in with strong activity deserves further study. Here we isolated identified two catechins from Zijuan tea, named zijuanin E (1) F (2). Their structures were by extensive high-resolution mass spectroscopy (HR-MS), nuclear magnetic resonance (NMR), ultraviolet-vis (UV),...
Through screening 50 strains of entomopathogenic fungi and rescreening 7 Paecilomyces gunnii, a methanol extract liquid-cultivated mycelia P. gunnii was found to have the strongest tyrosinase inhibitory activity. Preparative high-speed counter-current chromatography (HSCCC) guided by high-performance liquid (HPLC)-electrospray ionization (ESI)-high-resolution mass spectrometry (HRMS) employed for isolation purification active components, three new compounds with half inhibition concentration...
Zijuan tea ( Camellia sinensis var. assamica), an anthocyanin-rich cultivar with purple leaves, is a valuable material for manufacturing unique color and flavor. In this paper, four new phenylpropanoid substituted epicatechin gallates (pECGs), Zijuanins A-D (1-4), were isolated from green by different column chromatography. Their structures identified extensive high resolution mass spectroscopy (HR-MS), nuclear magnetic resonance (NMR), experimental calculated circular dichroism (CD)...
Genetic modification of Beauveria bassiana with the scorpion neurotoxin aaIT gene can distinctly increase its insecticidal activity, whereas effect this exogenous on metabolism B. is unknown until now. Thus, we investigate global metabolic profiling mycelia and conidia transgenic wild-type by liquid chromatography–mass spectrometry (LC–MS). Principal component analysis (PCA) orthogonal projection to latent structure discriminant (OPLS-DA) reveal clear discrimination from conidia. The...
Abstract Isaria cicadae is one of the fungi used in traditional Chinese medicine with longest tradition. It not only as a herbal but also health food Asia, together cultured cordyceps and mycelia fungus substitute. However, differences their metabolite are unknown. Using high‐performance liquid chromatography–mass spectrometry (HPLC–MS)‐based metabolomic method, we found that varies its metabolism during growth on wild insects, artificially raised insects artificial medium. There were 109...
The NGAL binding siderophore catechol was directly purified from human urine with columns and identified by LC-HR-ESI-MS, NMR spectroscopy methods.
Tea is an important beverage source of dietary polyphenols and well known for containing phenolic structure diversity. A series phenylpropanoid-substituted catechins, flavonols, flavan-3-hexoside, proanthocyanidin are present in different herbs with various biological activities, inspiring our exploration ester type catechins (PSECs) due to the enrichment galloylated tea. In this study, we used a guiding–screening–location–isolation integrated route including creating hypothesized PSEC...
Branched-chain amino acid (BCAA) metabolism plays a significant role in many biological activities beyond protein synthesis. Spore germination initiates the first stage of vegetative growth, which is critical for virulence pathogenic fungi. In this study, we demonstrated that keto-acid reductoisomerase MrILVC, key enzyme BCAA biosynthesis, from insect-pathogenic fungus Metarhizium robertsii associated with conidial and fungal pathogenicity. Surprisingly, Δ MrilvC mutant was restored when...
High-resolution mass spectrometry (HRMS) and nuclear magnetic resonance (NMR) analysis revealed that there are 20 main components in spores mycelia extract of Cordyceps fumosorosea strain RCEF 6672 including mannitol (1), uridine (2), adenine (3). N6-(2-hydroxyethyl)-adenosine (4). N6-(2-hydroxyethylacetate)-adenosine (5), fumosoroseanoside A (6) B (7), ovalicin-4α-alcohol (8), 1-linoleoyl-sn-glycero-3-phosphocholine (9) its isomer (10), fumosoroseain (11) (12), 5 non-ribosomal peptides (13...
Cordyceps chanhua is an important cordycipitoid mushroom widely used in Asia and beyond. Beauvericin (BEA), one of the bioactive compounds C. chanhua, has attracted much attention because its medicinal value food safety risk. In order to clear up relationship between oxidative stress BEA synthesis, we investigated impact H2O2-induced on secondary metabolism using untargeted metabolomics a transcript profiling approach. Metabolic mycelia found that total, 73 differential metabolites were...