A5068412241- CRISPR and Genetic Engineering
- Scientific Computing and Data Management
- Distributed and Parallel Computing Systems
- RNA Research and Splicing
- Fluid Dynamics and Turbulent Flows
- Animal Genetics and Reproduction
- Single-cell and spatial transcriptomics
- HIV Research and Treatment
- Genomics and Chromatin Dynamics
- Turbomachinery Performance and Optimization
- Food composition and properties
- Genetic Associations and Epidemiology
- Advanced Data Storage Technologies
- Neuroinflammation and Neurodegeneration Mechanisms
- Aerodynamics and Acoustics in Jet Flows
- Pluripotent Stem Cells Research
- Histone Deacetylase Inhibitors Research
- Big Data and Business Intelligence
- Machine Learning in Bioinformatics
- Infection Control and Ventilation
- Service and Product Innovation
- Cell Image Analysis Techniques
- Computational Fluid Dynamics and Aerodynamics
- Immune Cell Function and Interaction
- Muscle Physiology and Disorders
University of California, San Francisco
2015-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2025
University of Wisconsin–Madison
2024
Nanjing University of Chinese Medicine
2024
Changsha University
2021-2024
Chongqing Medical University
2024
Yunnan University
2020-2023
ShanghaiTech University
2023
Beijing Institute of Technology
2023
Guangzhou Medical University
2018-2022
Abstract The mammalian cerebrum performs high-level sensory perception, motor control and cognitive functions through highly specialized cortical subcortical structures 1 . Recent surveys of mouse human brains with single-cell transcriptomics 2–6 high-throughput imaging technologies 7,8 have uncovered hundreds neural cell types distributed in different brain regions, but the transcriptional regulatory programs that are responsible for unique identity function each type remain unknown. Here...
Human immunodeficiency virus type 1 (HIV-1) can establish latent infection following provirus integration into the host genome. NF-κB plays a critical role in activation of HIV-1 gene expression by cytokines and other stimuli, but signal transduction pathways that regulate switch from to productive have not been defined. Here, we show ERK1/ERK2 mitogen-activated protein kinase (MAPK) central linking signals at cell surface latently infected cells. MAPK was activated phorbol 12-myristate...
Abstract Cytosine base editors (CBEs) enable programmable C-to-T conversion without DNA double-stranded breaks and homology-directed repair in a variety of organisms, which exhibit great potential for agricultural biomedical applications. However, all reported cases only involved substitution at single targeted genomic site. Whether is effective multiple sites/loci has not been verified large animals. Here, by using pigs, an important animal agriculture biomedicine, as the subjective animal,...
ABSTRACT ERK1 and ERK2 mitogen-activated protein kinases (MAPK) play a critical role in regulation of cell proliferation differentiation response to mitogens other extracellular stimuli. Mitogens cytokines that activate MAPK T cells have been shown human immunodeficiency virus type 1 (HIV-1) replication. Little is known about the signal transduction pathways HIV-1 replication upon activation by stimulation. Here, we report through Ras/Raf/MEK signaling pathway enhances infectivity virions....
Pig cloning by somatic cell nuclear transfer (SCNT) remains extremely inefficient, and many cloned embryos undergo abnormal development. Here, profiling transcriptome expression, we observed dysregulated chromosome-wide gene expression in every chromosome identified a considerable number of genes that are aberrantly expressed the embryos. In particular, XIST, long non-coding RNA gene, showed high ectopic We also proved nullification XIST donor cells can normalize aberrant enhance long-term...
Abstract Amyloid‐ β (A ) toxicity is considered to be companioned by Tau phosphorylation in Alzheimer's disease (AD). The clinical AD therapy usually subjected low blood‐brain barrier (BBB) penetration and complex interaction mechanisms between A phosphorylated Tau. “Drug‐Carrier” synergy herein designed simultaneously target Tau‐associated pathways for treatment. To imitate natural nanoparticle configuration, the endogenous apolipoprotein A‐I its mimicking peptide 4F fused angiopep‐2 (Ang)...
Vif is a 23-kDa protein encoded by human immunodeficiency virus, type 1 (HIV-1) which important for virion infectivity. Here, we describe the phosphorylation of HIV-1 and its role in replication. In vivo studies demonstrated that highly phosphorylated on serine threonine residues. To identify sites characterize kinase(s), was expressed Escherichia coli purified use as substrate vitro kinase assays. The residues kinase(s) present both cytosol membrane fractions. Phosphorylation stimulated...
Grain chalkiness is a complex trait adversely affecting appearance and milling quality, therefore has been one of principal targets for rice improvement. Eliminating from daunting task due to the interaction between genotype environment lack molecular markers. In addition, mechanisms underlying grain formation are still imperfectly understood. We identified notched-belly mutant (DY1102) with high percentage white-belly, which only occurs in bottom part proximal embryo. Using this mutant,...
Brain ischemic preconditioning (PC) provides vital insights into the endogenous protection against stroke. Genomic and epigenetic responses to PC condition brain a state of tolerance. Notably, induces elevation histone acetylation, consistent with evidence that deacetylase (HDAC) inhibitors protect from injury. However, less is known about specific roles HDACs in this process. HDAC3 has been implicated several neurodegenerative conditions. Deletion confers neurotoxicity neuronal Here, we...
The human immunodeficiency virus type 1 (HIV-1) Vif protein plays a critical role in replication and infectivity. Here we show that is phosphorylated regulated by p44/42 mitogen-activated kinase (MAPK). phosphorylation MAPK was demonstrated <i>in vitro</i> as well vivo</i>and shown to occur on serine threonine residues. Two-dimensional tryptic phosphopeptide mapping indicated the same sites vivo</i>. Radioactive peptide sequencing identified two sites, Thr<sup>96</sup> Ser<sup>165</sup>....